PTPH1 (PTPN3) (NM_001145371) Human Tagged ORF Clone

CAT#: RC226916

PTPN3 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 3 (PTPN3), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPN3
• Synonyms: PTP-H1; PTPH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226916 representing NM_001145371
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCCCGGTTACGTGCGTTGGGTGGAAGAATTAATAATATACGCACCTCGGAGTTACCCAAAGAGA
  AAACTCGATCAGAAGTCATTTGCAGCATCCACTTTTTAGATGGCGTGGTACAGACCTTTAAAGTTACTAA
  ACAAGACACTGGCCAGGTTCTTCTGGATATGGTGCACAACCACCTGGGTGTGACTGAAAAGGAATATTTT
  GGTTTACAGCATGATGACGACTCCGTGGACTCTCCTAGATGGCTGGAAGCAAGCAAAGCCATCAGGAAGC
  AGTTAAAAGGAGGTTTCCCCTGTACCCTGCATTTTCGAGTAAGATTTTTTATACCTGATCCCAACACACT
  GCAGCAAGAACAAACCAGGCACTTGTATTTCTTACAACTGAAGATGGATATTTGCGAAGGAAGGTTAACC
  TGCCCTCTTAACTCAGCAGTGGTTCTAGCGTCCTATGCCGTACAATCTCATTTTGGAGACTATAATTCTT
  CCATACATCATCCAGGCTATCTTTCCGATAGTCACTTTATACCCGATCAAAATGAGGACTTTTTAACAAA
  AGTCGAATCTCTGCATGAGCAGCACAGTGGGCTAAAACAATCAGAAGCAGAATCCTGCTATATCAACATA
  GCGCGGACCCTCGACTTCTATGGAGTAGAACTGCACAGTGGTAGGGATCTGCACAATTTAGACCTAATGA
  TTGGAATTGCTTCCGCGGGTGTTGCTGTGTACCGAAAATACATTTGCACAAGTTTCTATCCTTGGGTGAA
  CATTCTCAAAATTTCTTTCAAAAGGAAAAAGTTCTTCATACATCAGCGACAGAAACAGGCTGAATCCAGG
  GAACATATTGTGGCCTTCAACATGCTGAATTACCGATCTTGCAAAAACTTGTGGAAATCCTGTGTTGAGC
  ACCATACGTTCTTTCAGGCAAAGAAGCTACTACCTCAGGAAAAGAATGTTCTGTCTCAGTACTGGACTAT
  GGGCTCTCGGAACACCAAAAAGTCGGTAAATAACCAATATTGCAAAAAGGTGATTGGCGGGATGGTGTGG
  AACCCAGCCATGCGGAGATCCTTATCAGTGGAGCACTTAGAAACCAAGAGTCTGCCTTCTCGTTCCCCTC
  CCATTACTCCCAACTGGCGAAGTCCTCGGCTCCGGCACGAAATCCGAAAGCCACGCCACTCTTCTGCAGA
  TAACCTTGCAAATGAAATGACCTACATCACGGAAACGGAAGATGTATTTTACACGTACAAGGGCTCTCTG
  GCCCCTCAAGACAGCGATTCTGAAGTTTCTCAGAACCGAAGCCCGCACCAAGAGAGTTTATCCGAGAACA
  ATCCGGCACAAAGCTACCTGACCCAGAAGTCATCCAGTTCTGTGTCTCCATCTTCAAATGCTCCAGGCTC
  CTGCTCACCTGACGGCGTTGATCAGCAGCTCTTAGATGACTTCCACAGGGTGACCAAAGGGGGCTCCACC
  GAGGACGCCAGCCAGTACTACTGTGACAAGAATGATAATGGTGACAGCTACTTAGTCTTGATCCGTATCA
  CACCAGATGAAGATGGAAAATTTGGATTTAATCTTAAGGGAGGAGTGGATCAAAAGATGCCTCTTGTGGT
  ATCAAGGATAAACCCAGAGTCACCTGCGGACACCTGCATTCCTAAGCTGAACGAAGGGGATCAAATCGTG
  TTAATCAATGGCCGGGACATCTCAGAACACACGCATGACCAAGTGGTGATGTTCATCAAAGCCAGCCGGG
  AGTCCCACTCACGGGAGCTGGCCCTGGTGATCAGGAGGAGAGCTGTCCGCTCATTTGCTGACTTCAAGTC
  TGAAGATGAACTGAACCAGCTTTTCCCCGAAGCCATTTTCCCCATGTGTCCGGAGGGTGGGGACACTTTG
  GAGGGATCCATGGCACAGCTAAAGAAGGGCCTCGAAAGCGGGACGGTGCTGATCCAGTTTGAGCAACTCT
  ACAGAAAAAAGCCAGGTTTGGCCATCACGTTTGCAAAGCTGCCTCAAAATTTGGACAAAAACCGATATAA
  AGATGTGCTGCCTTATGACACCACCCGGGTATTATTGCAGGGAAATGAAGATTATATTAATGCAAGTTAC
  GTGAACATGGAAATTCCTGCTGCTAACCTTGTGAACAAGTACATCGCCACTCAGGGGCCCCTGCCGCATA
  CCTGTGCACAGTTTTGGCAGGTTGTCTGGGATCAGAAGTTGTCACTCATTGTCATGTTGACGACTCTCAC
  AGAACGAGGGCGGACCAAATGTCACCAGTACTGGCCAGATCCCCCCGACGTCATGAACCACGGCGGCTTT
  CACATCCAGTGTCAGTCAGAGGACTGCACCATCGCCTATGTGTCCCGAGAAATGCTGGTCACAAACACCC
  AGACCGGGGAAGAACACACAGTGACACATCTCCAGTACGTCGCATGGCCTGACCACGGTGTGCCCGATGA
  CTCCTCCGACTTTCTGGAATTTGTAAACTATGTGAGGTCTCTGAGAGTGGACAGCGAGCCCGTCCTAGTT
  CACTGCAGTGCTGGAATAGGTCGAACCGGTGTGTTGGTCACTATGGAAACAGCCATGTGCCTAACTGAGA
  GGAACCTGCCCATTTACCCACTGGATATTGTCCGAAAAATGCGAGACCAGCGCGCCATGATGGTGCAGAC
  ATCAAGCCAGTACAAGTTTGTGTGTGAAGCGATTCTTCGTGTGTATGAAGAAGGTTTAGTCCAAATGCTG
  GATCCTAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226916 representing NM_001145371
  Red=Cloning site Green=Tags(s)
  
  MTSRLRALGGRINNIRTSELPKEKTRSEVICSIHFLDGVVQTFKVTKQDTGQVLLDMVHNHLGVTEKEYF
  GLQHDDDSVDSPRWLEASKAIRKQLKGGFPCTLHFRVRFFIPDPNTLQQEQTRHLYFLQLKMDICEGRLT
  CPLNSAVVLASYAVQSHFGDYNSSIHHPGYLSDSHFIPDQNEDFLTKVESLHEQHSGLKQSEAESCYINI
  ARTLDFYGVELHSGRDLHNLDLMIGIASAGVAVYRKYICTSFYPWVNILKISFKRKKFFIHQRQKQAESR
  EHIVAFNMLNYRSCKNLWKSCVEHHTFFQAKKLLPQEKNVLSQYWTMGSRNTKKSVNNQYCKKVIGGMVW
  NPAMRRSLSVEHLETKSLPSRSPPITPNWRSPRLRHEIRKPRHSSADNLANEMTYITETEDVFYTYKGSL
  APQDSDSEVSQNRSPHQESLSENNPAQSYLTQKSSSSVSPSSNAPGSCSPDGVDQQLLDDFHRVTKGGST
  EDASQYYCDKNDNGDSYLVLIRITPDEDGKFGFNLKGGVDQKMPLVVSRINPESPADTCIPKLNEGDQIV
  LINGRDISEHTHDQVVMFIKASRESHSRELALVIRRRAVRSFADFKSEDELNQLFPEAIFPMCPEGGDTL
  EGSMAQLKKGLESGTVLIQFEQLYRKKPGLAITFAKLPQNLDKNRYKDVLPYDTTRVLLQGNEDYINASY
  VNMEIPAANLVNKYIATQGPLPHTCAQFWQVVWDQKLSLIVMLTTLTERGRTKCHQYWPDPPDVMNHGGF
  HIQCQSEDCTIAYVSREMLVTNTQTGEEHTVTHLQYVAWPDHGVPDDSSDFLEFVNYVRSLRVDSEPVLV
  HCSAGIGRTGVLVTMETAMCLTERNLPIYPLDIVRKMRDQRAMMVQTSSQYKFVCEAILRVYEEGLVQML
  DPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145371
• ORF Size: 2742 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145371.1, NP_001138843.1
• RefSeq Size: 6217 bp
• RefSeq ORF: 1881 bp
• Locus ID: 5774
• Cytogenetics: 9q31.3
• Protein Families: Druggable Genome, Phosphatase
• MW: 104 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]'