GLG1 (NM_001145667) Human Tagged ORF Clone

CAT#: RC227066

GLG1 (Myc-DDK-tagged)-Human golgi glycoprotein 1 (GLG1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GLG1
• Synonyms: CFR-1; ESL-1; MG-160; MG160
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227066 representing NM_001145667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCGGCGTGTGGACGTGTACGGAGGATGTTCCGCTTGTCGGCGGCGCTGCATCTGCTGCTGCTATTCG
  CGGCCGGGGCCGAGAAACTCCCCGGCCAGGGCGTCCACAGCCAGGGCCAGGGTCCCGGGGCCAACTTTGT
  GTCCTTCGTAGGGCAGGCCGGAGGCGGCGGCCCGGCGGGTCAGCAGCTGCCCCAGCTGCCTCAGTCATCG
  CAGCTTCAGCAGCAACAGCAGCAGCAGCAACAGCAACAGCAGCCTCAGCCGCCGCAGCCGCCTTTCCCGG
  CGGGTGGGCCTCCGGCCCGGCGGGGAGGAGCGGGGGCTGGTGGGGGCTGGAAGCTGGCGGAGGAAGAGTC
  CTGCAGGGAGGACGTGACCCGCGTGTGCCCTAAGCACACCTGGAGCAACAACCTGGCGGTGCTCGAGTGC
  CTGCAGGATGTGAGGGAGCCTGAAAATGAAATTTCTTCAGACTGCAATCATTTGTTGTGGAATTATAAGC
  TGAACCTAACTACAGATCCCAAATTTGAATCTGTGGCCAGAGAGGTTTGCAAATCTACTATAACAGAGAT
  TAAAGAATGTGCTGATGAACCGGTTGGAAAAGGTTACATGGTTTCCTGCTTGGTGGATCACCGAGGCAAC
  ATCACTGAGTATCAGTGTCACCAGTACATTACCAAGATGACGGCCATCATTTTTAGTGATTACCGTTTAA
  TCTGTGGCTTCATGGATGACTGCAAAAATGACATCAACATTCTGAAATGTGGCAGTATTCGGCTTGGAGA
  AAAGGATGCACATTCACAAGGTGAGGTGGTATCATGCTTGGAGAAAGGCCTGGTGAAAGAAGCAGAAGAA
  AGAGAACCCAAGATTCAAGTTTCTGAACTCTGCAAGAAAGCCATTCTCCGGGTGGCTGAGCTGTCATCGG
  ATGACTTTCACTTAGACCGGCATTTATATTTTGCTTGCCGAGATGATCGGGAGCGTTTTTGTGAAAATAC
  ACAAGCTGGTGAGGGCAGAGTGTATAAGTGCCTCTTTAACCATAAATTTGAAGAATCCATGAGTGAAAAG
  TGTCGAGAAGCACTTACAACCCGCCAAAAGCTGATTGCCCAGGATTATAAAGTCAGTTATTCATTGGCCA
  AATCCTGTAAAAGTGACTTGAAGAAATACCGGTGCAATGTGGAAAACCTTCCGCGATCGCGTGAAGCCAG
  GCTCTCCTACTTGTTAATGTGCCTGGAGTCAGCTGTACACAGAGGGCGACAAGTCAGCAGTGAGTGCCAG
  GGGGAGATGCTGGATTACCGACGCATGTTGATGGAAGACTTTTCTCTGAGCCCTGAGATCATCCTAAGCT
  GTCGGGGGGAGATTGAACACCATTGTTCCGGATTACATCGAAAAGGGCGGACCCTACACTGTCTGATGAA
  AGTAGTTCGAGGGGAGAAGGGGAACCTTGGAATGAACTGCCAGCAGGCGCTTCAAACACTGATTCAGGAG
  ACTGACCCTGGTGCAGATTACCGCATTGATCGAGCTTTGAATGAAGCTTGTGAATCTGTAATCCAGACAG
  CCTGCAAACATATAAGATCTGGAGACCCAATGATCTTGTCGTGCCTGATGGAACATTTATACACAGAGAA
  GATGGTAGAAGACTGTGAACACCGTCTCTTAGAGCTGCAGTATTTCATCTCCCGGGATTGGAAGCTGGAC
  CCTGTCCTGTACCGCAAGTGCCAGGGAGACGCTTCTCGTCTTTGCCACACCCACGGTTGGAATGAGACCA
  GTGAATTTATGCCTCAGGGAGCTGTGTTCTCTTGTTTATACAGACACGCCTACCGCACTGAGGAACAGGG
  AAGGAGGCTCTCACGGGAGTGCCGAGCTGAAGTCCAAAGGATCCTACACCAGCGTGCCATGGATGTCAAG
  CTGGATCCTGCCCTCCAGGATAAGTGCCTGATTGATCTGGGAAAATGGTGCAGTGAGAAAACAGAGACTG
  GACAGGAGCTGGAGTGCCTTCAGGACCATCTGGATGACTTGGTGGTGGAGTGTAGAGATATAGTTGGCAA
  CCTCACTGAGTTAGAATCAGAGGATATTCAAATAGAAGCCTTGCTGATGAGAGCCTGTGAGCCCATAATT
  CAGAACTTCTGCCACGATGTGGCAGATAACCAGATAGACTCTGGGGACCTGATGGAGTGTCTGATACAGA
  ACAAACACCAGAAGGACATGAACGAGAAGTGTGCCATCGGAGTTACCCACTTCCAGCTGGTGCAGATGAA
  GGATTTTCGGTTTTCTTACAAGTTTAAAATGGCCTGCAAGGAGGACGTGTTGAAGCTTTGCCCAAACATA
  AAAAAGAAGGTGGACGTGGTGATCTGCCTGAGCACGACCGTGCGCAATGACACTCTGCAGGAAGCCAAGG
  AGCACAGGGTGTCCCTGAAGTGCCGCAGGCAGCTCCGTGTGGAGGAGCTGGAGATGACGGAGGACATCCG
  CTTGGAGCCAGATCTATACGAAGCCTGCAAGAGTGACATCAAAAACTTCTGTTCCGCTGTGCAATATGGC
  AACGCTCAGATTATCGAATGTCTGAAAGAAAACAAGAAGCAGCTAAGCACCCGCTGCCACCAAAAAGTAT
  TTAAGCTGCAGGAGACAGAGATGATGGACCCAGAGCTAGACTACACCCTCATGAGGGTCTGCAAGCAGAT
  GATAAAGAGGTTCTGTCCGGAAGCAGATTCTAAAACCATGTTGCAGTGCTTGAAGCAAAATAAAAACAGT
  GAATTGATGGATCCCAAATGCAAACAGATGATAACCAAGCGCCAGATCACCCAGAACACAGATTACCGCT
  TAAACCCCATGTTAAGAAAAGCCTGTAAAGCTGACATTCCTAAATTCTGTCACGGTATCCTGACTAAGGC
  CAAGGATGATTCAGAATTAGAAGGACAAGTCATCTCTTGCCTGAAGCTGAGATATGCTGACCAGCGCCTG
  TCTTCAGACTGTGAAGACCAGATCCGAATCATTATCCAGGAGTCCGCCCTGGACTACCGCCTGGATCCTC
  AGCTCCAGCTGCACTGCTCAGACGAGATCTCCAGTCTATGTGCTGAAGAAGCAGCAGCCCAAGAGCAGAC
  AGGTCAGGTGGAGGAGTGCCTCAAGGTCAACCTGCTCAAGATCAAAACAGAATTGTGTAAAAAGGAAGTG
  CTAAACATGCTGAAGGAAAGCAAAGCAGACATCTTTGTTGACCCGGTACTTCATACTGCTTGTGCCCTGG
  ACATTAAACACCACTGCGCAGCCATCACCCCTGGCCGCGGGCGTCAAATGTCCTGTCTCATGGAAGCACT
  GGAGGATAAGCGGGTGAGGTTACAGCCCGAGTGCAAAAAGCGCCTCAATGACCGGATTGAGATGTGGAGT
  TACGCAGCAAAGGTGGCCCCAGCAGATGGCTTCTCTGATCTTGCCATGCAAGTAATGACGTCTCCATCTA
  AGAACTACATTCTCTCTGTGATCAGTGGGAGCATCTGTATATTGTTCCTGATTGGCCTGATGTGTGGACG
  GATCACCAAGCGAGTGACACGAGAGCTCAAGGACAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227066 representing NM_001145667
  Red=Cloning site Green=Tags(s)
  
  MAACGRVRRMFRLSAALHLLLLFAAGAEKLPGQGVHSQGQGPGANFVSFVGQAGGGGPAGQQLPQLPQSS
  QLQQQQQQQQQQQQPQPPQPPFPAGGPPARRGGAGAGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLEC
  LQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTITEIKECADEPVGKGYMVSCLVDHRGN
  ITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINILKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEE
  REPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEK
  CREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQ
  GEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQE
  TDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLD
  PVLYRKCQGDASRLCHTHGWNETSEFMPQGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVK
  LDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLVVECRDIVGNLTELESEDIQIEALLMRACEPII
  QNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNI
  KKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRRQLRVEELEMTEDIRLEPDLYEACKSDIKNFCSAVQYG
  NAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNS
  ELMDPKCKQMITKRQITQNTDYRLNPMLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRL
  SSDCEDQIRIIIQESALDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEV
  LNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWS
  YAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145667
• ORF Size: 3537 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145667.1, NP_001139139.1
• RefSeq ORF: 3540 bp
• Locus ID: 2734
• Cytogenetics: 16q23.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 134.4 kDa
• Gene Summary: ''