SPDYA (NM_001142634) Human Tagged ORF Clone

CAT#: RC227335

SPDYA (Myc-DDK-tagged)-Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY428224) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227335 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPDYA
• Synonyms: RINGO3; RINGOA; SPDY1; SPY1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227335 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCACAATCAGATGTGTTGTGAGACACCACCTACTGTCACTGTTTATGTAAAATCAGGGTCAAATA
  GATCACATCAGCCTAAAAAGCCCATTACTCTGAAGCGTCCTATTTGTAAAGATAATTGGCAAGCATTTGA
  AAAAAATACACATAATAACAACAAATCTAAACGCCCCAAAGGACCTTGTCTGGTTATACAGCGTCAGGAT
  ATGACTGCTTTCTTTAAATTATTTGATGACGATTTAATTCAAGATTTCTTGTGGATGGACTGCTGCTGTA
  AAATTGCAGACAAGTATCTTTTGGCTATGACCTTTGTTTATTTCAAGAGGGCTAAATTTACTATAAGTGA
  GCATACCAGGATAAATTTCTTTATTGCTTTGTATCTGGCTAATACAGTTGAAGAAGATGAAGAAGAAACC
  AAGTACGAAATTTTTCCATGGGCTTTAGGGAAAAACTGGAGAAAATTGTTCCCTAATTTCTTAAAGTTAA
  GGGACCAGCTCTGGGATAGAATTGACTATAGGGCTATTGTAAGCAGGCGATGTTGTGAGGAGGTTATGGC
  CATTGCACCAACCCATTATATCTGGCAAAGAGAACGTTCTGTTCATCACAGTGGAGCTGTCAGAAACTAC
  AACAGAGATGAAGTTCAGCTGCCCCGGGGACCTAGTGCCACACCAGTAGATTGTTCACTCTGTGGTAAAA
  AAAGAAGATATGTTAGACTGGGATTGTCTTCATCATCATCTTTATCCAGTCATACAGCAGGGGTGACAGA
  AAAACATTCTCAGGACTCATACAACTCACTGTCAATGGACATAATAGGTGATCCTTCTCAAGCTTATACT
  GGTTCTGAAGTAGTCAATGACCATCAATCAAATAAAGGAAAGAAAACTAATTTCTTGAAGAAAGACAAAT
  CTATGGAGTGGTTTACAGGAAGTGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227335 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQD
  MTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEET
  KYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNY
  NRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYT
  GSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142634
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142634.1, NP_001136106.1
• RefSeq Size: 1756 bp
• RefSeq ORF: 942 bp
• Locus ID: 245711
• Protein Pathways: Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 36.5 kDa