DAXX (NM_001141970) Human Tagged ORF Clone

CAT#: RC227435

DAXX (Myc-DDK-tagged)-Human death-domain associated protein (DAXX), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY427987) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227435 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DAXX protein (Cat# TP327435). The protein was produced from HEK293T cells transfected with DAXX cDNA clone (Cat# RC227435) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAXX
• Synonyms: BING2; DAP6; EAP1; SMIM40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227435 representing NM_001141970
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGGTTCTGAGAATTGCGGCGAGGGTCGCCTCGAGAGACGGTTTCTGAGCATCATCGTGCTGGATG
  ATGATGACGAAGATGAAGCAGCTGCTCAGCCAGGGCCCTCCCACCCACTCCCCAATGCGGCCTCACCTGG
  GGCAGAAGCCCCTAGCTCCTCTGAGCCTCATGGGGCCAGAGGAAGCAGTAGTTCGGGCGGCAAGAAATGC
  TACAAGCTGGAGAATGAGAAGCTGTTCGAAGAGTTCCTTGAACTTTGTAAGATGCAGACAGCAGACCACC
  CTGAGGTGGTCCCATTCCTCTATAACCGGCAGCAACGTGCCCACTCTCTGTTTTTGGCCTCGGCGGAGTT
  CTGCAACATCCTCTCTAGGGTCCTGTCTCGGGCCCGGAGCCGGCCAGCCAAGCTCTATGTCTACATCAAT
  GAGCTCTGCACTGTTCTCAAGGCCCACTCAGCCAAAAAGAAGCTGAACTTGGCCCCTGCCGCCACCACCT
  CCAATGAGCCCTCTGGGAATAACCCTCCCACACACCTCTCCTTGGACCCCACAAATGCTGAAAACACTGC
  CTCTCAGTCTCCAAGGACCCGTGGTTCCCGGCGGCAGATCCAGCGTTTGGAGCAGCTGCTGGCGCTCTAT
  GTGGCAGAGATCCGGCGGCTGCAGGAAAAGGAGTTGGATCTCTCAGAATTGGATGACCCAGACTCCGCAT
  ACCTGCAGGAGGCACGGTTGAAGCGTAAGCTGATCCGCCTCTTTGGGCGACTATGTGAGCTGAAAGACTG
  CTCTTCACTGACCGGCCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGGTTAAC
  AGGCGCATTGAGCGGCTCATCAACAAGCCAGGGCCTGATACCTTCCCTGACTATGGGGATGTGCTTCGGG
  CTGTAGAGAAGGCAGCTGCCCGACACAGCCTTGGCCTCCCCCGACAGCAGCTCCAGCTCATGGCTCAGGA
  TGCCTTCCGAGATGTGGGCATCAGGTTACAGGAGCGACGTCACCTCGATCTCATCTACAACTTTGGCTGC
  CACCTCACAGATGACTATAGGCCAGGCGTTGACCCTGCACTATCAGATCCTGTGTTGGCCCGGCGCCTTC
  GGGAAAACCGGAGTTTGGCCATGAGTCGGCTGGATGAGGTCATCTCCAAATATGCAATGTTGCAAGACAA
  AAGTGAGGAGGGCGAGAGAAAAAAGAGAAGAGCTCGGCTCCAAGGCACCTCTTCCCACTCTGCAGACACC
  CCCGAAGCCTCCTTGGATTCTGGTGAGGGCCCTAGTGGAATGGCATCCCAGGGGTGCCCTTCTGCCTCCA
  GAGCTGAGACAGATGACGAAGACGATGAGGAGAGTGATGAGGAAGAGGAGGAGGAGGAGGAAGAAGAAGA
  GGAGGAGGCCACAGATTCTGAAGAGGAGGAGGATCTGGAACAGATGCAGGAGGGTCAGGAGGATGATGAA
  GAGGAGGACGAAGAGGAAGAAGCAGCAGCAGGTAAAGATGGAGACAAGAGCCCCATGTCCTCACTACAGA
  TCTCCAATGAAAAGAACCTGGAACCTGGCAAACAGATCAGCAGATCTTCAGGGGAGCAGCAAAACAAAGG
  ACGCATAGTGTCACCATCGTTACTGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAAT
  GGAGAACAGCCTGAGGAGCTGACCCTGGAGGAAGAAAGCCCTGTGTCTCAGCTCTTTGAGCTAGAGATTG
  AAGCTTTGCCCCTGGATACCCCTTCCTCTGTGGAGACGGACATTTCCTCTTCCAGGAAGCAATCAGAGGA
  GCCCTTCACCACTGTCTTAGAGAATGGAGCAGGCATGGTCTCTTCTACTTCCTTCAATGGAGGCGTCTCT
  CCTCACAACTGGGGAGATTCTGGTCCCCCCTGCAAAAAATCTCGGAAGGAGAAGAAGCAAACAGGATCAG
  GGCCATTAGGAAACAGCTATGTGGAAAGGCAAAGGTCAGTGCATGAGAAGAATGGGAAAAAGATATGTAC
  CCTGCCCAGCCCACCTTCCCCCTTGGCTTCCTTGGCCCCAGTTGCTGATTCCTCCACGAGGGTGGACTCT
  CCCAGCCATGGCCTGGTGACCAGCTCCCTCTGCATCCCTTCTCCAGCCCGGCTGTCCCAAACCCCCCATT
  CACAGCCTCCTCGGCCTGGTACTTGCAAGACAAGTGTGGCCACACAATGCGATCCAGAAGAGATCATCGT
  GCTCTCAGACTCTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227435 representing NM_001141970
  Red=Cloning site Green=Tags(s)
  
  MRGSENCGEGRLERRFLSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKC
  YKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYIN
  ELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALY
  VAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVN
  RRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGC
  HLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADT
  PEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDE
  EEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESN
  GEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVS
  PHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDS
  PSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001141970
• ORF Size: 2256 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001141970.1, NP_001135442.1
• RefSeq ORF: 2259 bp
• Locus ID: 1616
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway
• MW: 82.7 kDa
• Gene Summary: 'This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]'