DAXX (NM_001141970) Human Untagged Clone

CAT#: SC326221

DAXX (untagged)-Human death-domain associated protein (DAXX), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAXX
• Synonyms: BING2; DAP6; EAP1; SMIM40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001141970, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGAGGTTCTGAGAATTGCGGCGAGGGTCGCCTCGAGAGACGGTTTCTGAGCATCATCGTGCTGGATG
  ATGATGACGAAGATGAAGCAGCTGCTCAGCCAGGGCCCTCCCACCCACTCCCCAATGCGGCCTCACCTGG
  GGCAGAAGCCCCTAGCTCCTCTGAGCCTCATGGGGCCAGAGGAAGCAGTAGTTCGGGCGGCAAGAAATGC
  TACAAGCTGGAGAATGAGAAGCTGTTCGAAGAGTTCCTTGAACTTTGTAAGATGCAGACAGCAGACCACC
  CTGAGGTGGTCCCATTCCTCTATAACCGGCAGCAACGTGCCCACTCTCTGTTTTTGGCCTCGGCGGAGTT
  CTGCAACATCCTCTCTAGGGTCCTGTCTCGGGCCCGGAGCCGGCCAGCCAAGCTCTATGTCTACATCAAT
  GAGCTCTGCACTGTTCTCAAGGCCCACTCAGCCAAAAAGAAGCTGAACTTGGCCCCTGCCGCCACCACCT
  CCAATGAGCCCTCTGGGAATAACCCTCCCACACACCTCTCCTTGGACCCCACAAATGCTGAAAACACTGC
  CTCTCAGTCTCCAAGGACCCGTGGTTCCCGGCGGCAGATCCAGCGTTTGGAGCAGCTGCTGGCGCTCTAT
  GTGGCAGAGATCCGGCGGCTGCAGGAAAAGGAGTTGGATCTCTCAGAATTGGATGACCCAGACTCCGCAT
  ACCTGCAGGAGGCACGGTTGAAGCGTAAGCTGATCCGCCTCTTTGGGCGACTATGTGAGCTGAAAGACTG
  CTCTTCACTGACCGGCCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCACCCGCTACCCAGAGGTTAAC
  AGGCGCATTGAGCGGCTCATCAACAAGCCAGGGCCTGATACCTTCCCTGACTATGGGGATGTGCTTCGGG
  CTGTAGAGAAGGCAGCTGCCCGACACAGCCTTGGCCTCCCCCGACAGCAGCTCCAGCTCATGGCTCAGGA
  TGCCTTCCGAGATGTGGGCATCAGGTTACAGGAGCGACGTCACCTCGATCTCATCTACAACTTTGGCTGC
  CACCTCACAGATGACTATAGGCCAGGCGTTGACCCTGCACTATCAGATCCTGTGTTGGCCCGGCGCCTTC
  GGGAAAACCGGAGTTTGGCCATGAGTCGGCTGGATGAGGTCATCTCCAAATATGCAATGTTGCAAGACAA
  AAGTGAGGAGGGCGAGAGAAAAAAGAGAAGAGCTCGGCTCCAAGGCACCTCTTCCCACTCTGCAGACACC
  CCCGAAGCCTCCTTGGATTCTGGTGAGGGCCCTAGTGGAATGGCATCCCAGGGGTGCCCTTCTGCCTCCA
  GAGCTGAGACAGATGACGAAGACGATGAGGAGAGTGATGAGGAAGAGGAGGAGGAGGAGGAAGAAGAAGA
  GGAGGAGGCCACAGATTCTGAAGAGGAGGAGGATCTGGAACAGATGCAGGAGGGTCAGGAGGATGATGAA
  GAGGAGGACGAAGAGGAAGAAGCAGCAGCAGGTAAAGATGGAGACAAGAGCCCCATGTCCTCACTACAGA
  TCTCCAATGAAAAGAACCTGGAACCTGGCAAACAGATCAGCAGATCTTCAGGGGAGCAGCAAAACAAAGG
  ACGCATAGTGTCACCATCGTTACTGTCAGAAGAACCCCTGGCCCCCTCCAGCATAGATGCTGAAAGCAAT
  GGAGAACAGCCTGAGGAGCTGACCCTGGAGGAAGAAAGCCCTGTGTCTCAGCTCTTTGAGCTAGAGATTG
  AAGCTTTGCCCCTGGATACCCCTTCCTCTGTGGAGACGGACATTTCCTCTTCCAGGAAGCAATCAGAGGA
  GCCCTTCACCACTGTCTTAGAGAATGGAGCAGGCATGGTCTCTTCTACTTCCTTCAATGGAGGCGTCTCT
  CCTCACAACTGGGGAGATTCTGGTCCCCCCTGCAAAAAATCTCGGAAGGAGAAGAAGCAAACAGGATCAG
  GGCCATTAGGAAACAGCTATGTGGAAAGGCAAAGGTCAGTGCATGAGAAGAATGGGAAAAAGATATGTAC
  CCTGCCCAGCCCACCTTCCCCCTTGGCTTCCTTGGCCCCAGTTGCTGATTCCTCCACGAGGGTGGACTCT
  CCCAGCCATGGCCTGGTGACCAGCTCCCTCTGCATCCCTTCTCCAGCCCGGCTGTCCCAAACCCCCCATT
  CACAGCCTCCTCGGCCTGGTACTTGCAAGACAAGTGTGGCCACACAATGCGATCCAGAAGAGATCATCGT
  GCTCTCAGACTCTGATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001141970
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001141970.1, NP_001135442.1
• RefSeq Size: 2563 bp
• RefSeq ORF: 2259 bp
• Locus ID: 1616
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway
• Gene Summary: 'This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]'
  Transcript Variant: This variant (3) lacks an in-frame segment in a 5' coding exon and uses an upstream start codon, compared to variant 1. The resulting protein (isoform b) has a distinct and longer N-terminus, compared to isoform a.