STRA6 (NM_001142618) Human Tagged ORF Clone

CAT#: RC227472

STRA6 (Myc-DDK-tagged)-Human stimulated by retinoic acid gene 6 homolog (mouse) (STRA6), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY428212) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227472 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STRA6
• Synonyms: MCOPCB8; MCOPS9; PP14296
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227472 representing NM_001142618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCCAGCCAGCAGGGAACCAGACCTCCCCCGGGGCCACAGAGGACTACTCCTATGGCAGCTGGT
  ACATCGATGAGCCCCAGGGGGGCGAGGAGCTCCAGCCAGAGGGGGAAGTGCCCTCCTGCCACACCAGCAT
  ACCACCCGGCCTGTACCACGCCTGCCTGGCCTCGCTGTCAATCCTTGTGCTGCTGCTCCTGGCCATGCTG
  GTGAGGCGCCGCCAGCTCTGGCCTGACTGTGTGCGTGGCAGGCCCGGCCTGCCCAGCCCTGTGGATTTCT
  TGGCTGGGGACAGGCCCCGGGCAGTGCCTGCTGCTGTTTTCATGGTCCTCCTGAGCTCCCTGTGTTTGCT
  GCTCCCCGACGAGGACGCATTGCCCTTCCTGACTCTCGCCTCAGCACCCAGCCAAGATGGGAAAACTGAG
  GCTCCAAGAGGGGCCTGGAAGATACTGGGACTGTTCTATTATGCTGCCCTCTACTACCCTCTGGCTGCCT
  GTGCCACGGCTGGCCACACAGCTGCACACCTGCTCGGCAGCACGCTGTCCTGGGCCCACCTTGGGGTCCA
  GGTCTGGCAGAGGGCAGAGTGTCCCCAGGTGCCCAAGATCTACAAGTACTACTCCCTGCTGGCCTCCCTG
  CCTCTCCTGCTGGGCCTCGGATTCCTGAGCCTTTGGTACCCTGTGCAGCTGGTGAGAAGCTTCAGCCGTA
  GGACAGGAGCAGGCTCCAAGGGGCTGCAGAGCAGCTACTCTGAGGAATATCTGAGGAACCTCCTTTGCAG
  GAAGAAGCTGGGAAGCAGCTACCACACCTCCAAGCATGGCTTCCTGTCCTGGGCCCGCGTCTGCTTGAGA
  CACTGCATCTACACTCCACAGCCAGGATTCCATCTCCCGCTGAAGCTGGTGCTTTCAGCTACACTGACAG
  GGACGGCCATTTACCAGGTGGCCCTGCTGCTGCTGGTGGGCGTGGTACCCACTATCCAGAAGGTGAGGGC
  AGGGGTCACCACGGATGTCTCCTACCTGCTGGCCAGCTTTGGAATCGTGCTCTCCGAGGACAAGCAGGAG
  GTGGTGGAGCTGGTGAAGCACCATCTGTGGGCTCTGGAAGTGTGCTACATCTCAGCCTTGGTCTTGTCCT
  GCTTACTCACCTTCCTGGTCCTGATGCGCTCACTGGTGACACACAGGACCAACCTTCGAGCTCTGCACCG
  AGGAGCTGCCCTGGACTTGAGTCCCTTGCATCGGAGTCCCCATCCCTCCCGCCAAGCCATATTCTGTTGG
  ATGAGCTTCAGTGCCTACCAGACAGCCTTTATCTGCCTTGGGCTCCTGGTGCAGCAGATCATCTTCTTCC
  TGGGAACCACGGCCCTGGCCTTCCTGGTGCTCATGCCTGTGCTCCATGGCAGGAACCTCCTGCTCTTCCG
  TTCCCTGGAGTCCTCGTGGCCCTTCTGGCTGACTTTGGCCCTGGCTGTGATCCTGCAGAACATGGCAGCC
  CATTGGGTCTTCCTGGAGACTCATGATGGACACCCACAGCTGACCAACCGGCGAGTGCTCTATGCAGCCA
  CCTTTCTTCTCTTCCCCCTCAATGTGCTGGTGGGTGCCATGGTGGCCACCTGGCGAGTGCTCCTCTCTGC
  CCTCTACAACGCCATCCACCTTGGCCAGATGGACCTCAGCCTGCTGCCACCGAGAGCCGCCACTCTCGAC
  CCCGGCTACTACACGTACCGAAACTTCTTGAAGATTGAAGTCAGCCAGTCGCATCCAGCCATGACAGCCT
  TCTGCTCCCTGCTCCTGCAAGCGCAGAGCCTCCTACCCAGGACCATGGCAGCCCCCCAGGACAGCCTCAG
  ACCAGGGGAGGAAGACGAAGGGATGCAGCTGCTACAGACAAAGGACTCCATGGCCAAGGGAGCTAGGCCC
  GGGGCCAGCCGCGGCAGGGCTCGCTGGGGTCTGGCCTACACGCTGCTGCACAACCCAACCCTGCAGGTCT
  TCCGCAAGACGGCCCTGTTGGGTGCCAATGGTGCCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227472 representing NM_001142618
  Red=Cloning site Green=Tags(s)
  
  MSSQPAGNQTSPGATEDYSYGSWYIDEPQGGEELQPEGEVPSCHTSIPPGLYHACLASLSILVLLLLAML
  VRRRQLWPDCVRGRPGLPSPVDFLAGDRPRAVPAAVFMVLLSSLCLLLPDEDALPFLTLASAPSQDGKTE
  APRGAWKILGLFYYAALYYPLAACATAGHTAAHLLGSTLSWAHLGVQVWQRAECPQVPKIYKYYSLLASL
  PLLLGLGFLSLWYPVQLVRSFSRRTGAGSKGLQSSYSEEYLRNLLCRKKLGSSYHTSKHGFLSWARVCLR
  HCIYTPQPGFHLPLKLVLSATLTGTAIYQVALLLLVGVVPTIQKVRAGVTTDVSYLLASFGIVLSEDKQE
  VVELVKHHLWALEVCYISALVLSCLLTFLVLMRSLVTHRTNLRALHRGAALDLSPLHRSPHPSRQAIFCW
  MSFSAYQTAFICLGLLVQQIIFFLGTTALAFLVLMPVLHGRNLLLFRSLESSWPFWLTLALAVILQNMAA
  HWVFLETHDGHPQLTNRRVLYAATFLLFPLNVLVGAMVATWRVLLSALYNAIHLGQMDLSLLPPRAATLD
  PGYYTYRNFLKIEVSQSHPAMTAFCSLLLQAQSLLPRTMAAPQDSLRPGEEDEGMQLLQTKDSMAKGARP
  GASRGRARWGLAYTLLHNPTLQVFRKTALLGANGAQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142618
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142618.1, NP_001136090.1
• RefSeq ORF: 2004 bp
• Locus ID: 64220
• Protein Families: Transmembrane
• MW: 73.3 kDa
• Gene Summary: The protein encoded by this gene is a membrane protein involved in the metabolism of retinol. The encoded protein acts as a receptor for retinol/retinol binding protein complexes. This protein removes the retinol from the complex and transports it across the cell membrane. Defects in this gene are a cause of syndromic microphthalmia type 9 (MCOPS9). Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]