RBM17 (NM_001145547) Human Tagged ORF Clone

CAT#: RC227492

RBM17 (Myc-DDK-tagged)-Human RNA binding motif protein 17 (RBM17), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428938) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227492 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBM17
• Synonyms: SPF45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227492 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTGTACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCCAAAAACTTCA
  AACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCAAAGAGCCAAAGGACGAAACA
  AAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGTGGCTCCTCAGATGACCGGCAAATTGTGGAC
  ACTCCACCGCATGTAGCAGCTGGGCTGAAGGATCCTGTTCCCAGTGGGTTTTCTGCAGGGGAAGTTCTGA
  TTCCCTTAGCTGACGAATATGACCCTATGTTTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGA
  GGAACGACAGAGACAGCGGGAGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGAC
  AGACATGAAGCAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGATTATGAGCGAG
  AGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGAAAGACAAAGA
  GTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCTCGATCACAGTCTTCCAAAGCAGCCATTCCT
  CCCCCAGTGTACGAGGAACAAGACAGACCGAGATCTCCAACCGGACCTAGCAACTCCTTCCTCGCTAACA
  TGGGGGGCACGGTGGCGCACAAGATCATGCAGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCA
  TGAGCAGGGCCTGAGCACTGCCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGC
  GACGCCACAGAGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTTAAGTGTCCTA
  CTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGGGAGGTGGATGAAGACTTGGAAGTTGAAACCAA
  GGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTGATATTTGAAATTCCTGGTGCCCCTGATGAT
  GAAGCAGTACGGATATTTTTAGAATTTGAGAGAGTTGAATCAGCAATTAAAGCGGTTGTTGACTTGAATG
  GGAGGTATTTTGGTGGACGGGTGGTAAAAGCATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTT
  GGCAGAACAAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227492 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVD
  TPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKD
  RHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIP
  PPVYEEQDRPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVG
  DATEKDASKKSDSNPLTEILKCPTKVVLLRNMVGAGEVDEDLEVETKEECEKYGKVGKCVIFEIPGAPDD
  EAVRIFLEFERVESAIKAVVDLNGRYFGGRVVKACFYNLDKFRVLDLAEQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145547
• ORF Size: 1203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145547.1, NP_001139019.1
• RefSeq Size: 3760 bp
• RefSeq ORF: 1206 bp
• Locus ID: 84991
• Protein Pathways: Spliceosome
• MW: 45 kDa
• Gene Summary: This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009]