Emi1 (FBXO5) (NM_001142522) Human Tagged ORF Clone

CAT#: RC227496

FBXO5 (Myc-DDK-tagged)-Human F-box protein 5 (FBXO5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXO5
• Synonyms: EMI1; FBX5; Fbxo31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227496 representing NM_001142522
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGTGATTTTAATTGTAACCATGTTCATTCCGGACTTAAACTGGTAAAACCTGATGACATTGGAA
  GACTAGTTTCCTACACCCCTGCATATTTGGAAGGTTCCTGTAAAGACTGCATTAAAGACTATGAAAGGCT
  GTCATGTATTGGGTCACCGATTGTGAGCCCTAGGATTGTACAACTTGAAACTGAAAGCAAGCGCTTGCAT
  AACAAGGAAAATCAACATGTGCAACAGACACTTAATAGTACAAATGAAATAGAAGCACTAGAGACCAGTA
  GACTTTATGAAGACAGTGGCTATTCCTCATTTTCTCTACAAAGTGGCCTCAGTGAACATGAAGAAGGTAG
  CCTCCTGGAGGAGAATTTCGGTGACAGTCTACAATCCTGCCTGCTACAAATACAAAGCCCAGACCAATAT
  CCCAACAAAAACTTGCTGCCAGTTCTTCATTTTGAAAAAGTGGTTTGTTCAACATTAAAAAAGAATGCAA
  AACGAAATCCTAAAGTAGATCGGGAGATGCTGAAGGAAATTATAGCCAGAGGAAATTTTAGACTGCAGAA
  TATAATTGGCAGAAAAATGGGCCTAGAATGTGTAGATATTCTCAGCGAACTCTTTCGAAGGGGACTCAGA
  CATGTCTTAGCAACTATTTTAGCACAACTCAGTGACATGGACTTAATCAATGTGTCTAAAGTGAGCACAA
  CTTGGAAGAAGATCCTAGAAGATGATAAGGGGGCATTCCAGTTGTACAGTAAAGCAATACAAAGAGTTAC
  CGAAAACAACAATAAATTTTCACCTCATGCTTCAACCAGAGAATATGTTATGTTCAGAACCCCACTGGCT
  TCTGTTCAGAAATCAGCAGCCCAGACTTCTCTCAAAAAAGATGCTCAAACCAAGTTATCCAATCAAGGTG
  ATCAGAAAGGTTCTACTTATAGTCGACACAATGAATTCTCTGAGGTTGCCAAGACATTGAAAAAGAACGA
  AAGCCTCAAAGCCTGTATTCGCTGTAATTCACCTGCAAAATATGATTGCTATTTACAACGGGCAACCTGC
  AAACGAGAAGGCTGTGGATTTGATTATTGTACGAAGTGTCTCTGTAATTATCATACTACTAAAGACTGTT
  CAGATGGCAAGCTCCTCAAAGCCAGTTGTAAAATAGGTCCCCTGCCTGGTACAAAGAAAAGCAAAAAGAA
  TTTACGAAGATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227496 representing NM_001142522
  Red=Cloning site Green=Tags(s)
  
  MKCDFNCNHVHSGLKLVKPDDIGRLVSYTPAYLEGSCKDCIKDYERLSCIGSPIVSPRIVQLETESKRLH
  NKENQHVQQTLNSTNEIEALETSRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGDSLQSCLLQIQSPDQY
  PNKNLLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMGLECVDILSELFRRGLR
  HVLATILAQLSDMDLINVSKVSTTWKKILEDDKGAFQLYSKAIQRVTENNNKFSPHASTREYVMFRTPLA
  SVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHNEFSEVAKTLKKNESLKACIRCNSPAKYDCYLQRATC
  KREGCGFDYCTKCLCNYHTTKDCSDGKLLKASCKIGPLPGTKKSKKNLRRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142522
• ORF Size: 1203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142522.1, NM_001142522.2, NP_001135994.1
• RefSeq Size: 2269
• RefSeq ORF: 1206
• Locus ID: 26271
• Protein Families: Druggable Genome
• Protein Pathways: Oocyte meiosis
• MW: 45.4 kDa
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]