EXOC7 (NM_001145298) Human Tagged ORF Clone

CAT#: RC227511

EXOC7 (Myc-DDK-tagged)-Human exocyst complex component 7 (EXOC7), transcript variant 5

Product Images

Western blot validation of overexpression lysate (Cat# LY428802) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227511 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOC7
• Synonyms: 2-5-3p; BLOM4; EX070; EXO70; Exo70p; EXOC1; YJL085W
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227511 representing NM_001145298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCCCCACAGGAGGCATCCGCTCGACGGCGGGAGATTGAGGACAAGCTGAAGCAGGAGGAGGAGA
  CTCTGTCCTTCATCCGAGACAGCCTGGAGAAGAGCGACCAGCTCACTAAGAACATGGTGTCTATCTTATC
  ATCCTTTGAGAGCCGCCTTATGAAGCTGGAGAACTCCATCATCCCTGTGCACAAGCAGACGGAGAATCTG
  CAGCGGCTGCAGGAGAATGTTGAGAAGACGCTGTCCTGCCTGGACCATGTCATCAGCTACTACCATGTGG
  CCAGTGACACTGAGAAGATCATCAGAGAGGGCCCCACAGGTAGGCTGGAAGAGTACCTGGGAAGCATGGC
  CAAGATTCAGAAGGCAGTGGAGTATTTCCAGGACAACAGCCCAGACAGCCCGGAACTCAACAAAGTGAAA
  CTGCTCTTTGAGCGCGGGAAGGAGGCCCTGGAGTCCGAATTTCGCAGCCTGATGACGCGGCACAGTAAGG
  TCGTCTCGCCCGTGCTCATCTTGGATCTGATCAGTGGTGACGATGATCTGGAGGCCCAGGAGGACGTGAC
  CCTGGAGCACCTGCCCGAGAGCGTGCTCCAGGATGTCATTCGCATCTCCCGCTGGCTGGTGGAATATGGC
  CGCAACCAAGATTTCATGAACGTCTACTACCAGATACGCTCCAGCCAGCTGGACCGCTCCATCAAAGGAC
  TGAAGGAGCATTTCCATAAGAGCAGTTCTTCCTCTGGGGTTCCCTACTCCCCTGCTATCCCCAACAAGAG
  GAAAGACACACCTACCAAGAAGCCAGTCAAGCGGCCAGGTCACGAGCATGATTTCCGAGTTAAGCACCTG
  TCCGAGGCCTTGAACGACAAGCACGGGCCGCTGGCCGGGAGAGATGACATGCTGGACGTGGAGACCGATG
  CCTACATCCACTGCGTCAGTGCCTTCGTCAAGCTGGCGCAGAGCGAGTACCAGCTGCTGGCCGACATCAT
  CCCCGAGCACCACCAGAAGAAGACCTTCGACTCCCTGATACAGGATGCCCTGGATGGGCTGATGCTTGAA
  GGGGAGAACATCGTGTCTGCTGCCCGGAAGGCCATTGTGCGACACGACTTCTCCACGGTGCTCACCGTCT
  TCCCCATCCTGCGACACCTCAAGCAGACCAAGCCTGAGTTTGACCAGGTGCTCCAGGGCACGGCTGCCAG
  CACAAAGAACAAGCTGCCTGGCCTCATCACATCCATGGAGACCATCGGTGCCAAAGCGCTGGAGGACTTC
  GCAGACAACATCAAGAATGACCCGGACAAGGAGTACAACATGCCGAAGGACGGCACCGTACACGAGCTCA
  CCAGCAATGCCATCCTCTTCCTGCAGCAGCTTTTGGACTTCCAGGAGACGGCAGGCGCCATGCTGGCCTC
  CCAAGAGACCAGCTCTTCGGCCACCAGCTACAGCTCTGAGTTCAGCAAGCGGCTGCTAAGCACCTATATC
  TGTAAAGTGCTGGGCAACCTGCAGTTGAACTTGCTGAGCAAGTCCAAGGTGTACGAGGACCCAGCTCTGA
  GCGCCATCTTCCTGCACAACAACTACAATTACATCCTCAAGTCCCTGGAGAAGTCTGAACTGATCCAGCT
  GGTGGCAGTGACACAGAAGACTGCTGAGCGCTCCTACCGGGAGCACATTGAGCAGCAGATCCAGACCTAC
  CAGCGCAGCTGGTTAAAGGTGACTGATTACATCGCAGAGAAGAATCTACCTGTGTTCCAGCCGGGAGTCA
  AGCTCCGGGACAAGGAGCGGCAGATTATCAAGGAGCGTTTTAAGGGCTTCAATGATGGCCTCGAAGAACT
  GTGCAAAATCCAGAAGGCCTGGGCTATTCCAGACACAGAGCAGAGGGACAGGATTCGCCAGGCCCAGAAG
  ACCATTGTCAAGGAGACCTACGGGGCCTTTCTACAGAAGTTTGGCAGCGTGCCCTTCACCAAGAACCCGG
  AGAAGTACATCAAGTACGGGGTGGAGCAGGTGGGCGACATGATCGATCGCCTTTTCGACACCTCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227511 representing NM_001145298
  Red=Cloning site Green=Tags(s)
  
  MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENL
  QRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVK
  LLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYG
  RNQDFMNVYYQIRSSQLDRSIKGLKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGHEHDFRVKHL
  SEALNDKHGPLAGRDDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDGLMLE
  GENIVSAARKAIVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGTAASTKNKLPGLITSMETIGAKALEDF
  ADNIKNDPDKEYNMPKDGTVHELTSNAILFLQQLLDFQETAGAMLASQETSSSATSYSSEFSKRLLSTYI
  CKVLGNLQLNLLSKSKVYEDPALSAIFLHNNYNYILKSLEKSELIQLVAVTQKTAERSYREHIEQQIQTY
  QRSWLKVTDYIAEKNLPVFQPGVKLRDKERQIIKERFKGFNDGLEELCKIQKAWAIPDTEQRDRIRQAQK
  TIVKETYGAFLQKFGSVPFTKNPEKYIKYGVEQVGDMIDRLFDTSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145298
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145298.1, NM_001145298.2, NM_001145298.3, NP_001138770.1
• RefSeq ORF: 2031
• Locus ID: 23265
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway
• MW: 77.1 kDa
• Gene Summary: The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Nov 2011]