OTUD5 (NM_001136158) Human Tagged ORF Clone

CAT#: RC227589

OTUD5 (Myc-DDK-tagged)-Human OTU domain containing 5 (OTUD5), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY427831) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227589 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OTUD5
• Synonyms: DUBA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227589 representing NM_001136158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGCCAACGAGCCGCCGCCGCCCG
  GGCCGATGCCCCCGGCGCCGCGGCGCGGCGGAGGTGTGGGCGTGGGCGGCGGCGGCACGGGCGTGGGCGG
  CGGCGATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCG
  CTACCAGGACCGCCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCC
  GGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGACGCGCT
  GGGCGCAGCGGCGGCGGGTGTGGGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGC
  GGCTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGGTTGGCGCGGTTGGCGGGG
  GCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGCTACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACG
  CATCGAGGCTATGGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTACGAGACAAG
  AAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGTCTCTTCCGGGCTGTAGCTGACCAGGTGT
  ATGGAGACCAGGACATGCATGAGGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTA
  CTTCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGGAAAAACAATTGCCATGGC
  AACCACATTGAGATGCAGGCCATGGCAGAGATGTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAG
  AACCCATCAACACATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGTTAGCTACCATCGGAA
  TATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCATTGGTGTGGGGCTGGGCCTGCCATCATTC
  AAACCAGGGTTTGCAGAGCAGTCTCTGATGAAGAATGCCATAAAAACATCGGAGGAGTCATGGATTGAAC
  AGCAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGAAGCCATCGAGGAGCAGGT
  GGCTCGGGAATCCTACCTGCAGTGGTTGCGGGATCAGGAGAAACAGGCTCGCCAGGTCCGAGGCCCCAGC
  CAGCCCCGGAAAGCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTGGCCTGGAGGAGTGGA
  CTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTGAGCACCCTGAGCTGCATGCTGAATTGGG
  CATGAAGCCCCCTTCCCCAGGCACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCCTGTGCGCCAGGTACA
  AGCAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCCTCTACCCTGCTTTGGAGT
  GCCGGGCCCTCATTCAGCAGATGTCCCCCTCTGCCTTTGGTCTGAATGACTGGGATGATGATGAGATCCT
  AGCTTCGGTGCTGGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGCACAGAGAC
  CCGCCCCCAGACAAGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227589 representing NM_001136158
  Red=Cloning site Green=Tags(s)
  
  MTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGP
  LPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVG
  GCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDK
  KGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHG
  NHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSF
  KPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPS
  QPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGT
  SSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVHRD
  PPPDKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136158
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001136158.1, NP_001129630.1
• RefSeq ORF: 1701
• Locus ID: 55593
• Protein Families: Protease
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 60 kDa
• Gene Summary: This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]