DACH2 (NM_001139514) Human Tagged ORF Clone

CAT#: RC227672

DACH2 (Myc-DDK-tagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY427975) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227672 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DACH2
• Synonyms: FLJ31391; MGC138545
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227672 representing NM_001139514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTCTCCGCATCTCCAGTGATCTCTGCAACTTCCAGCGGCGCCGGCGTCCCGGGGGGCTTATTCC
  GGGCCGAACCCCTGTACTCGACTCCCAGAGAGCCCCCTCGTCTTACTCCTAATATGATCAATAGTTTCGT
  GGTTAATAACCACAGCAACAGTGCCGGAGGCGGCGGCAGGGGCAACACCAACACCAACGAGTGCCGCATG
  GTCGACATGCACGGGATGAAGGTGGCTTCGTTCCTGATGGACGGCCAGGAACTGATCTGCCTGCCGCAAG
  TCTTTGATCTTTTTCTCAAGCACCTGGTGGGAGGCTTGCACACTGTGTACACCAAGCTGAAGAGACTGGA
  TATATCCCCCGTGGTGTGTACTGTTGAGCAGGTCCGGATCCTCCGCGGGCTGGGGGCCATCCAGCCCGGG
  GTAAACCGCTGCAAACTCATCACCAGGAAAGACTTCGAAACTTTGTTCACCGATTGCACCAATGCCAGTT
  CAAGACCCGGCAGGCCCCCTAAGCGTTCTTTGGGAGTGTTGCAGGAAAATGCCCGCCTTCTGACCCATGC
  AGTCCCAGGCCTCTTATCGCCAGGACTTATCACTCCGACAGGTATAACAGCTGCAGCGATGGCTGAGGCG
  ATGAAACTTCAGAAGATGAAGCTTATGGCTATGAACACTCTTCAGGGAAATGGAAGCCAAAATGGGACCG
  AATCAGAGCCTGATGATCTTAATTCTAACACAGGTGGAAGTGAATCCTCCTGGGATAAAGATAAGATGCA
  GTCTCCATTTGCTGCACCTGGACCCCAACATGGAATTGCTCATGCAGCCCTAGCTGGCCAGCCAGGCATT
  GGGGGTGCTCCAACCCTCAATCCACTGCAGCAGAACCACCTGCTAACCAATAGACTGGATCTGCCATTTA
  TGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGTTGCCATGGCAATGAATCAGAT
  GAACCATCTCAATACTATTGCCAACATGGCTGCTGCAGCACAGATTCACAGTCCACTCTCCAGAGCTGGT
  ACCTCTGTTATAAAGGAGCGGATCCCAGAGAGTCCTTCTCCTGCTCCTTCTCTAGAAGAGAATCATCGTC
  CTGGGAGCCAGACCTCTTCCCACACCAGCAGCAGTGTGTCCAGCTCTCCCTCTCAGATGGATCATCATTT
  GGAAAGAATGGAAGAGGTACCAGTTCAAATTCCAATAATGAAGTCACCCTTGGACAAGATACAGCTGACT
  CCTGGGCAGGCATTGCCCGCTGGATTCCCTGGACCATTCATTTTTGCTGATAGTCTGTCCTCCGTGGAGA
  CTCTGTTGACCAACATTCAGGGTCTGCTGAAAGTTGCTTTGGATAATGCTCGCATCCAGGAGAAGCAGAT
  TCAACAAGAAAAGAAGGAGCTGCGACTGGAGCTCTATAGAGAGAGAGAAATTAGAGAAAACCTTGAAAGA
  CAACTTGCAGTTGAGCTTCAAAGCAGAACTACTATGCAAAAGCGCCTGAAGAAGGAGAAAAAAACCAAGA
  GAAAATTGCAGGAAGCCTTGGAATTTGAATCAAAGCGCCGGGAGCAAGTGGAGCAGGCACTTAAGCAAGC
  CACCACTAGTGACAGTGGCCTGAGGATGTTAAAAGATACTGGAATTCCAGATATTGAAATAGAAAACAAT
  GGGACTCCTCATGATAGTGCTGCTATGCAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227672 representing NM_001139514
  Red=Cloning site Green=Tags(s)
  
  MAVSASPVISATSSGAGVPGGLFRAEPLYSTPREPPRLTPNMINSFVVNNHSNSAGGGGRGNTNTNECRM
  VDMHGMKVASFLMDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDISPVVCTVEQVRILRGLGAIQPG
  VNRCKLITRKDFETLFTDCTNASSRPGRPPKRSLGVLQENARLLTHAVPGLLSPGLITPTGITAAAMAEA
  MKLQKMKLMAMNTLQGNGSQNGTESEPDDLNSNTGGSESSWDKDKMQSPFAAPGPQHGIAHAALAGQPGI
  GGAPTLNPLQQNHLLTNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMAAAAQIHSPLSRAG
  TSVIKERIPESPSPAPSLEENHRPGSQTSSHTSSSVSSSPSQMDHHLERMEEVPVQIPIMKSPLDKIQLT
  PGQALPAGFPGPFIFADSLSSVETLLTNIQGLLKVALDNARIQEKQIQQEKKELRLELYREREIRENLER
  QLAVELQSRTTMQKRLKKEKKTKRKLQEALEFESKRREQVEQALKQATTSDSGLRMLKDTGIPDIEIENN
  GTPHDSAAMQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001139514
• ORF Size: 1713 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001139514.1, NP_001132986.1
• RefSeq ORF: 1716
• Locus ID: 117154
• Protein Families: Transcription Factors
• MW: 61.8 kDa
• Gene Summary: This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]