YIF1B (NM_001145462) Human Tagged ORF Clone

CAT#: RC227675

YIF1B (Myc-DDK-tagged)-Human Yip1 interacting factor homolog B (S. cerevisiae) (YIF1B), transcript variant 8

Product Images

Western blot validation of overexpression lysate (Cat# LY428905) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227675 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: YIF1B
• Synonyms: FinGER8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227675 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCGGCAGGCTTGGCGGCGGCGGCTGCGGGGACGCCCCGGCTGCGTAAGTGGCCCTCGAAGCGGA
  GGATCCCTGTGTCCCAGCCGGGCATGGCCGACCCCCACCAGCTTTTCGATGACACAAGTTCAGCCCAGAG
  CCGGGGCTATGGGGCCCAGCGGGCACCTGGTGGCCTGAGTTATCCTGCAGCCTCTCCCACGCCCCATGCA
  GCCTTCCTGGCTGACCCGGTGTCCAACATGGCCATGGCCTATGGGAGCAGCCTGGCCGCGCAGGGCAAGG
  AGCTGGTGGATAAGAACATCGACCGCTTCATCCCCATCACCAAGCTCAAGTATTACTTTGCTGTGGACAC
  CATGTATGTGGGCAGAAAGCTGGGCCTGCTGTTCTTCCCCTACCTACACCAGGACTGGGAAGTGCAGTAC
  CAACAGGACACCCCGGTGGCCCCCCGCTTTGACGTCAATGCCCCGGACCTCTACATTCCAGCAATGGCTT
  TCATCACCTACGTTTTGGTGGCTGGTCTTGCGCTGGGGACCCAGGATAGGTTCTCCCCAGACCTCCTGGG
  GCTGCAAGCGAGCTCAGCCCTGGCCTGGCTGACCCTGGAGGTGCTGGCCATCCTGCTCAGCCTCTATCTG
  GTCACTGTCAACACCGACCTCACCACCATCGACCTGGTGGCCTTCTTGGGCTACAAATATGTCGGGATGA
  TTGGCGGGGTCCTCATGGGCCTGCTCTTCGGGAAGATTGGCTACTACCTGGTGCTGGGCTGGTGCTGCGT
  AGCCATCTTTGTGTTCATGATCCGGACGCTGCGGCTGAAGATCTTGGCAGACGCAGCAGCTGAGGGGGTC
  CCGGTGCGTGGGGCCCGGAACCAGCTGCGCATGTACCTGACCATGGCGGTGGCGGCGGCGCAGCCTATGC
  TCATGTACTGGCTCACCTTCCACCTGGTGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227675 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPAGLAAAAAGTPRLRKWPSKRRIPVSQPGMADPHQLFDDTSSAQSRGYGAQRAPGGLSYPAASPTPHA
  AFLADPVSNMAMAYGSSLAAQGKELVDKNIDRFIPITKLKYYFAVDTMYVGRKLGLLFFPYLHQDWEVQY
  QQDTPVAPRFDVNAPDLYIPAMAFITYVLVAGLALGTQDRFSPDLLGLQASSALAWLTLEVLAILLSLYL
  VTVNTDLTTIDLVAFLGYKYVGMIGGVLMGLLFGKIGYYLVLGWCCVAIFVFMIRTLRLKILADAAAEGV
  PVRGARNQLRMYLTMAVAAAQPMLMYWLTFHLVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145462
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145462.1, NP_001138934.1
• RefSeq Size: 2764 bp
• RefSeq ORF: 852 bp
• Locus ID: 90522
• Protein Families: Transmembrane
• MW: 34.4 kDa