PSMB5 (NM_001144932) Human Tagged ORF Clone
CAT#: RC227745L3
- LentiORF®
Lenti-ORF clone of PSMB5 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, beta type, 5 (PSMB5), transcript variant 3
"NM_001144932" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PSMB5 |
Synonyms | LMPX; MB1; X |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC227745).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001144932 |
ORF Size | 609 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001144932.1, NP_001138404.1 |
RefSeq ORF | 612 bp |
Locus ID | 5693 |
Cytogenetics | 14q11.2 |
Protein Families | Protease |
Protein Pathways | Proteasome |
MW | 21.7 kDa |
Gene Summary | 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC325599 | PSMB5 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 5 (PSMB5), transcript variant 3 |
USD 420.00 |
|
RC227745 | PSMB5 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, beta type, 5 (PSMB5), transcript variant 3 |
USD 420.00 |
|
RG227745 | PSMB5 (GFP-tagged) - Human proteasome (prosome, macropain) subunit, beta type, 5 (PSMB5), transcript variant 3 |
USD 460.00 |
|
RC227745L4 | Lenti-ORF clone of PSMB5 (mGFP-tagged)-Human proteasome (prosome, macropain) subunit, beta type, 5 (PSMB5), transcript variant 3 |
USD 620.00 |
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