Apoptosis inhibitor 5 (API5) (NM_001142931) Human Tagged ORF Clone
CAT#: RC227863
API5 (Myc-DDK-tagged)-Human apoptosis inhibitor 5 (API5), transcript variant 3
"NM_001142931" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | API5 |
Synonyms | AAC-11; AAC11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227863 representing NM_001142931
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGACAGTAGAGGAGCTTTACCGCAATTATGGCATCCTGGCCGATGCCACGGAGCAAGTGGGCCAGA TTCGACGTCAAGCAATTAAAGAACTGCCTCAATTTGCCACTGGAGAAAATCTTCCTCGAGTGGCAGATAT ACTAACGCAACTTTTGCAGACAGATGACTCTGCAGAATTTAACCTAGTGAACAATGCCCTATTAAGTATA TTTAAAATGGATGCAAAAGGGACTTTAGGTGGGTTGTTCAGCCAAATACTTCAAGGAGAGGACATTGTTA GAGAACGAGCAATTAAATTCCTTTCTACAAAACTTAAGACTTTACCAGATGAAGTCTTAACAAAGGAAGT GGAAGAGCTTATACTAACTGAATCCAAAAAGGTCCTAGAAGATGTGACTGGTGAAGAATTTGTTCTATTT ATGAAGATACTGTCTGGGTTAAAAAGCTTACAGACAGTGAGTGGAAGACAGCAACTTGTAGAGTTGGTGG CTGAACAGGCCGACCTAGAACAGACCTTCAATCCCTCGGATCCTGACTGTGTGGACAGGCTCTTACAGTG CACTCGGCAGGCAGTACCCCTCTTCTCTAAAAATGTCCATTCCACAAGGTTTGTGACATATTTCTGTGAG CAGGTTCTCCCTAACCTCGGTACCTTGACTACCCCAGTGGAAGGTCTTGATATACAGTTGGAGGTATTGA AATTGTTGGCGGAGATGAGTTCATTTTGTGGTGACATGGAAAAACTAGAAACAAATTTAAGGAAACTATT TGATAAGTTATTGGAATACATGCCCCTCCCTCCAGAAGAGGCAGAAAATGGAGAGAATGCTGGTAATGAA GAACCCAAGCTACAGTTCAGTTATGTGGAATGTTTGTTGTACAGTTTTCACCAGTTGGGCCGAAAACTTC CAGATTTCTTAACAGCCAAACTGAATGCAGAAAAGCTCAAAGATTTCAAAATCAGGCTGCAGTACTTTGC ACGGGGCCTGCAAGTTTATATCAGACAACTTCGCTTAGCTCTCCAGGGTAAAACGGGTGAGGCCTTAAAA ACAGAAGAGAACAAGATTAAAGTCGTTGCATTGAAAATAACAAACAATATCAATGTTTTAATCAAGGATC TCTTCCACATTCCTCCTTCTTATAAGAGCACAGTAACACTATCCTGGAAACCTGTACAAAAGGTTGAGAT TGGGCAAAAGAGAGCCAGTGAAGATACAACTTCAGGTTCACCACCCAAGAAATCTTCAGCAGGACCAAAA AGAGATGCCAGGCAGATTTATAACCCTCCCAGTGGGAAATATAGCAGCAATTTGGGCAACTTTAATTATG AGAGGAGCCTTCAGGGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227863 representing NM_001142931
Red=Cloning site Green=Tags(s) MPTVEELYRNYGILADATEQVGQIRRQAIKELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSI FKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEELILTESKKVLEDVTGEEFVLF MKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCE QVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNE EPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALK TEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPK RDARQIYNPPSGKYSSNLGNFNYERSLQGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142931 |
ORF Size | 1350 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142931.1, NP_001136403.1 |
RefSeq ORF | 1353 |
Locus ID | 8539 |
Protein Families | Druggable Genome |
MW | 50.6 kDa |
Gene Summary | This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC325947 | API5 (untagged)-Human apoptosis inhibitor 5 (API5), transcript variant 3 |
USD 760.00 |
|
RG227863 | API5 (GFP-tagged) - Human apoptosis inhibitor 5 (API5), transcript variant 3 |
USD 460.00 |
|
RC227863L3 | Lenti ORF clone of Human apoptosis inhibitor 5 (API5), transcript variant 3, Myc-DDK-tagged |
USD 620.00 |
|
RC227863L4 | Lenti ORF clone of Human apoptosis inhibitor 5 (API5), transcript variant 3, mGFP tagged |
USD 620.00 |
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