Apoptosis inhibitor 5 (API5) (NM_001142931) Human Tagged ORF Clone

CAT#: RC227863

API5 (Myc-DDK-tagged)-Human apoptosis inhibitor 5 (API5), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY428299) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227863 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified API5 protein (Cat# TP327863). The protein was produced from HEK293T cells transfected with API5 cDNA clone (Cat# RC227863) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: API5
• Synonyms: AAC-11; AAC11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227863 representing NM_001142931
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACAGTAGAGGAGCTTTACCGCAATTATGGCATCCTGGCCGATGCCACGGAGCAAGTGGGCCAGA
  TTCGACGTCAAGCAATTAAAGAACTGCCTCAATTTGCCACTGGAGAAAATCTTCCTCGAGTGGCAGATAT
  ACTAACGCAACTTTTGCAGACAGATGACTCTGCAGAATTTAACCTAGTGAACAATGCCCTATTAAGTATA
  TTTAAAATGGATGCAAAAGGGACTTTAGGTGGGTTGTTCAGCCAAATACTTCAAGGAGAGGACATTGTTA
  GAGAACGAGCAATTAAATTCCTTTCTACAAAACTTAAGACTTTACCAGATGAAGTCTTAACAAAGGAAGT
  GGAAGAGCTTATACTAACTGAATCCAAAAAGGTCCTAGAAGATGTGACTGGTGAAGAATTTGTTCTATTT
  ATGAAGATACTGTCTGGGTTAAAAAGCTTACAGACAGTGAGTGGAAGACAGCAACTTGTAGAGTTGGTGG
  CTGAACAGGCCGACCTAGAACAGACCTTCAATCCCTCGGATCCTGACTGTGTGGACAGGCTCTTACAGTG
  CACTCGGCAGGCAGTACCCCTCTTCTCTAAAAATGTCCATTCCACAAGGTTTGTGACATATTTCTGTGAG
  CAGGTTCTCCCTAACCTCGGTACCTTGACTACCCCAGTGGAAGGTCTTGATATACAGTTGGAGGTATTGA
  AATTGTTGGCGGAGATGAGTTCATTTTGTGGTGACATGGAAAAACTAGAAACAAATTTAAGGAAACTATT
  TGATAAGTTATTGGAATACATGCCCCTCCCTCCAGAAGAGGCAGAAAATGGAGAGAATGCTGGTAATGAA
  GAACCCAAGCTACAGTTCAGTTATGTGGAATGTTTGTTGTACAGTTTTCACCAGTTGGGCCGAAAACTTC
  CAGATTTCTTAACAGCCAAACTGAATGCAGAAAAGCTCAAAGATTTCAAAATCAGGCTGCAGTACTTTGC
  ACGGGGCCTGCAAGTTTATATCAGACAACTTCGCTTAGCTCTCCAGGGTAAAACGGGTGAGGCCTTAAAA
  ACAGAAGAGAACAAGATTAAAGTCGTTGCATTGAAAATAACAAACAATATCAATGTTTTAATCAAGGATC
  TCTTCCACATTCCTCCTTCTTATAAGAGCACAGTAACACTATCCTGGAAACCTGTACAAAAGGTTGAGAT
  TGGGCAAAAGAGAGCCAGTGAAGATACAACTTCAGGTTCACCACCCAAGAAATCTTCAGCAGGACCAAAA
  AGAGATGCCAGGCAGATTTATAACCCTCCCAGTGGGAAATATAGCAGCAATTTGGGCAACTTTAATTATG
  AGAGGAGCCTTCAGGGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227863 representing NM_001142931
  Red=Cloning site Green=Tags(s)
  
  MPTVEELYRNYGILADATEQVGQIRRQAIKELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSI
  FKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEELILTESKKVLEDVTGEEFVLF
  MKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTRFVTYFCE
  QVLPNLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYMPLPPEEAENGENAGNE
  EPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALK
  TEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPK
  RDARQIYNPPSGKYSSNLGNFNYERSLQGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142931
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142931.1, NP_001136403.1
• RefSeq ORF: 1353
• Locus ID: 8539
• Protein Families: Druggable Genome
• MW: 50.6 kDa
• Gene Summary: This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]