SH2B1 (NM_001145796) Human Tagged ORF Clone

CAT#: RC227887

SH2B1 (Myc-DDK-tagged)-Human SH2B adaptor protein 1 (SH2B1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH2B1
• Synonyms: PSM; SH2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227887 representing NM_001145796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGCCCCTTCCCCAGAGGACGGGGCCTCCCCCTCGTCTCCCCCGCTGCCCCCACCCCCGCCCC
  CTAGTTGGCGGGAGTTCTGTGAGTCCCACGCCCGGGCTGCGGCTCTGGACTTTGCCCGCCGTTTTCGCCT
  CTACCTGGCCTCCCACCCCCAATATGCGGGGCCCGGGGCCGAGGCTGCCTTCTCCCGCCGTTTTGCTGAG
  CTCTTCCTGCAGCACTTTGAAGCCGAGGTGGCCCGGGCCTCTGGCTCCCTGTCGCCACCCATCCTGGCTC
  CCCTGAGCCCTGGTGCGGAGATTTCGCCACATGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCCCCT
  GGCTGTGCTGGGCCCTTCTCGATCATCTGAGGACCTGGCCGGCCCCCTCCCTTCCTCAGTCTCTTCCTCC
  TCTACAACCTCCTCCAAGCCGAAGCTCAAGAAGCGCTTTTCCCTGCGTTCAGTGGGTCGCTCTGTCCGAG
  GCTCAGTCCGTGGCATCCTGCAGTGGCGGGGGACCGTTGACCCTCCCTCCTCCGCTGGGCCCCTGGAGAC
  CTCGTCAGGCCCCCCAGTCTTAGGTGGAAACAGCAACTCCAACTCCTCTGGCGGGGCTGGGACCGTTGGT
  AGGGGACTGGTCAGTGATGGAACGTCCCCTGGGGAAAGATGGACTCACCGTTTTGAGAGGCTGAGACTCA
  GTCGGGGAGGGGGCGCCTTGAAGGATGGAGCAGGGATGGTGCAGAGGGAAGAGCTGCTGAGTTTCATGGG
  GGCTGAGGAGGCAGCCCCTGACCCAGCCGGAGTGGGCCGGGGAGGAGGGGTGGCTGGGCCTCCTTCAGGG
  GGAGGAGGGCAGCCTCAGTGGCAGAAGTGTCGCCTGCTGCTTCGAAGTGAAGGAGAAGGAGGAGGAGGAA
  GTCGCCTGGAGTTCTTTGTACCACCCAAGGCCTCTCGGCCCCGACTCAGCATCCCCTGCTCTTCTATCAC
  AGACGTCCGGACAACCACAGCCCTGGAGATGCCTGACCGGGAGAACACGTTTGTGGTTAAGGTGGAAGGT
  CCATCCGAGTATATCATGGAGACAGTGGATGCCCAGCATGTGAAGGCCTGGGTGTCTGACATCCAAGAAT
  GCCTGAGCCCAGGACCCTGCCCTGCTACCAGTCCCCGCCCCATGACCCTCCCTCTGGCCCCTGGGACCTC
  ATTCCTTACAAGGGAGAACACAGACAGCCTGGAGCTGTCCTGCCTGAATCACTCGGAGAGTCTACCCAGC
  CAGGACCTGCTGCTTGGACCCAGCGAGAGCAATGACCGCCTGTCGCAGGGGGCATATGGGGGCCTCTCAG
  ACCGCCCCTCGGCATCCATCTCCCCCAGCTCTGCCTCCATTGCCGCCTCCCATTTTGACTCGATGGAACT
  GCTTCCCCCAGAGTTGCCCCCCCGCATCCCCATTGAAGAGGGACCCCCAACAGGGACAGTTCATCCCCTC
  TCAGCCCCCTACCCTCCCTTGGACACTCCGGAAACAGCCACAGGGTCCTTCCTGTTCCAGGGGGAGCCAG
  AGGGCGGTGAGGGGGACCAGCCCCTCTCAGGGTATCCTTGGTTCCACGGGATGCTCTCTCGGCTCAAGGC
  TGCACAGTTGGTGCTGACTGGCGGCACTGGCTCCCACGGTGTCTTCCTGGTGCGCCAGAGTGAGACAAGG
  CGGGGTGAATACGTCCTCACCTTCAACTTCCAGGGCAAGGCCAAGCACCTGCGTTTGTCGCTGAACGAGG
  AGGGTCAGTGCCGGGTCCAGCACCTGTGGTTCCAGTCCATTTTCGATATGCTCGAGCACTTCCGGGTGCA
  CCCCATCCCTTTGGAGTCGGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTCCCATCCTCCCAGCGA
  CAGCAGGGCCGGGAGCAGGCTGGGAGCCATGCGGGGGTGTGCGAGGGAGATGGATGCCACCCCGATGCCT
  CCTGCACCCTCATGCCCTTCGGAGCGAGTGACTGTGTAACCGACCACCTCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227887 representing NM_001145796
  Red=Cloning site Green=Tags(s)
  
  MNGAPSPEDGASPSSPPLPPPPPPSWREFCESHARAAALDFARRFRLYLASHPQYAGPGAEAAFSRRFAE
  LFLQHFEAEVARASGSLSPPILAPLSPGAEISPHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVSSS
  STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGTVDPPSSAGPLETSSGPPVLGGNSNSNSSGGAGTVG
  RGLVSDGTSPGERWTHRFERLRLSRGGGALKDGAGMVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSG
  GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEG
  PSEYIMETVDAQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPS
  QDLLLGPSESNDRLSQGAYGGLSDRPSASISPSSASIAASHFDSMELLPPELPPRIPIEEGPPTGTVHPL
  SAPYPPLDTPETATGSFLFQGEPEGGEGDQPLSGYPWFHGMLSRLKAAQLVLTGGTGSHGVFLVRQSETR
  RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSSQR
  QQGREQAGSHAGVCEGDGCHPDASCTLMPFGASDCVTDHLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145796
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145796.1, NP_001139268.1
• RefSeq ORF: 2016
• Locus ID: 25970
• Protein Pathways: Neurotrophin signaling pathway
• MW: 70.6 kDa
• Gene Summary: This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]