RPEL1 (NM_001143909) Human Tagged ORF Clone

CAT#: RC227890

LOC729020 (Myc-DDK-tagged)-Human rcRPE (LOC729020)

Product Images

Western blot validation of overexpression lysate (Cat# LY428400) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227890 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPEL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227890 representing NM_001143909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGGCTGCAAGATTGGCCCGTCCATCCTCAACAGCGACCTGGCCAATTTAGGGGCCAAGTGCC
  TCCAGATGCTAGACTCTGGGGCCGATTATCTGCATCTGGATGTAATGGATGGGCATTTTGTTCCCAACAT
  CACCTTTGGTCACCCTGTGGTAGAAAGCCTTCGAAAGCAGCTAGGCCAGGACCCTTTCTTTGACATGCAC
  ATGATGGTGTCCAAGCCAGAACAGTGGGTAAAGCCAATGGCTGTAGCAGAAGCCAATCAGTACACCTTTC
  ATCTCGAGGCTACTGAGAACCCAGGGACTTTGATTAAAGACATTCGGGAGAATGGGATGAAGGTTGGCCT
  TGCCATCAAACCAGGAACCTCAGTTGAGTATTTGGCACCATGGGCTAATCAAATAGATATGGCCTTGGTT
  ATGACAGTGGAACCGGGGTTTGGAGAGCAGAAATTCATGGAAGATATGATGCCAAAGGTTCACTGGTTGA
  GGACCCAGTTCCCATCTTTGGATATAGAGGGCGATGGTGGAGTAGGTTCTGACACTGTCCATAAGTGTGC
  AGAGGCAGGAGCTAACATGACTGTATCTGGCAGTGCTATTATGAGGAGTGAAGACCCCAGATCTGTGATC
  AACCTATTAAGAAATATTTGCTCAGAAGCTGCTCAGAAACGTTCTCTTGATCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227890 representing NM_001143909
  Red=Cloning site Green=Tags(s)
  
  MASGCKIGPSILNSDLANLGAKCLQMLDSGADYLHLDVMDGHFVPNITFGHPVVESLRKQLGQDPFFDMH
  MMVSKPEQWVKPMAVAEANQYTFHLEATENPGTLIKDIRENGMKVGLAIKPGTSVEYLAPWANQIDMALV
  MTVEPGFGEQKFMEDMMPKVHWLRTQFPSLDIEGDGGVGSDTVHKCAEAGANMTVSGSAIMRSEDPRSVI
  NLLRNICSEAAQKRSLDR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143909
• ORF Size: 684 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143909.1, NP_001137381.1
• RefSeq ORF: 687
• Locus ID: 729020
• Protein Pathways: Metabolic pathways, Pentose and glucuronate interconversions, Pentose phosphate pathway
• MW: 24.8 kDa