ENDOGL1 (EXOG) (NM_001145464) Human Tagged ORF Clone

CAT#: RC227897

EXOG (Myc-DDK-tagged)-Human endo/exonuclease (5'-3'), endonuclease G-like (EXOG), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428907) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227897 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOG
• Synonyms: ENDOGL1; ENDOGL2; ENGL; ENGL-a; ENGL-b; ENGLA; ENGLB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227897 representing NM_001145464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATCAAGAGTATCGCTTCCCGCCTCCGGGGTTCCCGTCGTTTTCTGAGCGGCTTCGTGGCTGGGG
  CTGTAGTGGGCGCTGCGGGAGCTGGGCTCGCGGCCCTGCAGTTCTTCCGGAGTCAGGGCGCTGAGGGAGC
  GTTGACAGGGAAGCAGCCGGATGGTGATGCAGACAGAAAGCATTGTAAATTTAAGCCTGATCCCAATATC
  CCTCCAACCTTCAGTGCCTTCAATGAAGATTATGTTGGAAGTGGGTGGTCACGAGGACACATGGCTCCAG
  CAGGAAATAACAAATTTTCAAGTAAAGCCATGGCTGAAACCTTTTACCTTTCTAACATTGTGCCTCAGGA
  TTTTGATAATAATTCTGGATATTGGAACAGAATAGAAATGTACTGTCGAGAGCTGACAGAAAGGTTTGAA
  GATGTTTGGGTGGTATCTGGGCCTTTGACCTTACCTCAGACTAGAGGCGATGGAAAGAAAATAGTTAGTT
  ACCAGGTGATTGGCGAGGACAACGTGGCAGTCCCCTCACACCTTTATAAGGTAATCCTGGCCCGCAGAAG
  CTCAGTATCTACCGAACCACTGGCGCTAGGGGCCTTTGTGGTACCCAATGAAGCCATCGGCTTCCAGCCC
  CAGTTAACTGAATTCCAAGTGAGCCTCCAGGACCTAGAGAAGTTGTCAGGACTGGTGTTTTTTCCTCATT
  TGGATAGAACTAGTGATATCCGGAATATCTGCTCTGTGGACACCTGTAAGCTCCTGGATTTCCAGGAGTT
  CACCTTGTACTTGAGTACAAGAAAGATTGAAGGAGCCCGATCAGTGCTCAGACTGGAAAAGATCATGGAA
  AACTTGAAGAATGCAGAGATTGAACCAGATGATTACTTTATGAGTCGCTATGAGAAGAAGCTAGAAGAAC
  TCAAAGCTAAGGAGCAGTCAGGAACCCAGATAAGAAAGCCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227897 representing NM_001145464
  Red=Cloning site Green=Tags(s)
  
  MAIKSIASRLRGSRRFLSGFVAGAVVGAAGAGLAALQFFRSQGAEGALTGKQPDGDADRKHCKFKPDPNI
  PPTFSAFNEDYVGSGWSRGHMAPAGNNKFSSKAMAETFYLSNIVPQDFDNNSGYWNRIEMYCRELTERFE
  DVWVVSGPLTLPQTRGDGKKIVSYQVIGEDNVAVPSHLYKVILARRSSVSTEPLALGAFVVPNEAIGFQP
  QLTEFQVSLQDLEKLSGLVFFPHLDRTSDIRNICSVDTCKLLDFQEFTLYLSTRKIEGARSVLRLEKIME
  NLKNAEIEPDDYFMSRYEKKLEELKAKEQSGTQIRKPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145464
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145464.1, NP_001138936.1
• RefSeq ORF: 957
• Locus ID: 9941
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis
• MW: 35.44 kDa
• Gene Summary: This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18. [provided by RefSeq, Feb 2009]