OGDHL (NM_001143996) Human Tagged ORF Clone

CAT#: RC227996

OGDHL (Myc-DDK-tagged)-Human oxoglutarate dehydrogenase-like (OGDHL), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OGDHL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227996 representing NM_001143996
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAGCTGAGGCTGCTGCCGTCCCGTCTTGGGGTACAGGCTGCGAGGCTCCTGGCTGCACATGACG
  TCCCGGTGTTTGGCTGGCGCAGCAGGTCCTCCGGGCCACCGGCCACCTTCCCAAGCAGCAAAGGTGGAGG
  CGGCTCCAGTTACATGGAGGAGATGTACTTCGCCTGGTTGGAAAACCCCCAGAGTGTCCACAAGATCCGG
  GGTCACCATGTGGCCCAGCTGGACCCCCTGGGCATTCTGGATGCAGACCTGGACTCCTTTGTGCCCTCAG
  ACTTGATCACAACCATTGATAAACTGGCCTTCTATGACCTTCAGGAGGCTGACCTTGATAAGGAGTTCCA
  GCTGCCGACAACCACCTTCATTGGGGGCTCTGAAAACACCCTCTCTCTGCGGGAGATCATTCGGCGCCTG
  GAGAACACCTACTGCCAGCACATTGGCCTGGAGTTCATGTTCATCAACGATGTGGAGCAGTGCCAGTGGA
  TCCGGCAGAAGTTTGAGACCCCTGGTGTGATGCAGTTCTCCAGCGAGGAGAAGCGGACCCTGCTGGCCCG
  GCTAGTGCGCTCCATGAGGTTTGAAGACTTCCTGGCCCGGAAATGGTCCTCAGAGAAGCGGTTTGGCCTG
  GAGGGCTGTGAAGTGATGATTCCTGCCCTCAAGACCATCATCGACAAATCCAGCGAGATGGGGATTGAGA
  ATGTCATCTTGGGGATGCCACACAGGGGAAGGCTGAACGTGCTGGCCAACGTGATCCGCAAGGACCTGGA
  GCAGATCTTCTGCCAGTTTGACCCCAAGCTGGAGGCGGCGGACGAGGGCTCCGGGGATGTCAAGTACCAC
  CTGGGCATGTACCATGAGAGGATCAACCGCGTCACCAACCGGAACATCACTCTGTCGCTGGTTGCCAACC
  CCTCCCACCTGGAGGCAGTGGACCCTGTGGTGCAGGGGAAGACAAAGGCAGAGCAGTTCTACCGTGGAGA
  TGCCCAGGGCAAGAAGGTCATGTCCATCCTGGTTCATGGGGACGCCGCCTTTGCTGGCCAGGGCGTGGTA
  TATGAGACCTTCCACCTGAGCGACCTGCCCTCCTACACGACCAATGGTACCGTGCACGTCGTCGTCAACA
  ACCAGATTGGATTCACCACAGACCCCCGAATGGCCCGCTCCTCACCATACCCGACCGACGTGGCCCGGGT
  GGTCAATGCGCCTATCTTCCATGTGAATGCCGATGACCCAGAGGCTGTGATATATGTGTGCAGTGTGGCA
  GCCGAATGGAGAAACACTTTCAACAAAGATGTTGTCGTGGACCTGGTCTGTTACCGCCGGCGTGGCCACA
  ATGAGATGGACGAGCCCATGTTCACCCAGCCGCTCATGTACAAGCAGATCCACAGACAGGTGCCTGTGCT
  GAAGAAGTACGCAGACAAGCTGATTGCCGAGGGCACAGTCACCCTGCAGGAGTTTGAGGAAGAAATTGCC
  AAATACGACCGGATCTGTGAGGAGGCTTATGGCAGGTCCAAGGATAAAAAGATTCTGCATATAAAGCACT
  GGTTGGACTCCCCCTGGCCTGGCTTCTTCAACGTAGATGGGGAGCCCAAGAGCATGACATGCCCAGCCAC
  GGGGATCCCTGAGGACATGCTCACCCACATCGGCAGTGTGGCCAGCTCTGTGCCCCTGGAGGACTTTAAG
  ATCCACACTGGCCTCTCTCGCATTCTGCGGGGCCGTGCGGACATGACCAAGAACCGGACGGTGGACTGGG
  CGTTGGCAGAGTACATGGCCTTTGGCTCCCTGCTGAAGGAAGGCATCCACGTGCGGCTCAGCGGGCAGGA
  TGTGGAGAGGGGCACATTCAGTCACCGGCACCATGTTCTCCATGACCAGGAGGTTGACCGCAGGACGTGT
  GTGCCTATGAATCATCTCTGGCCTGACCAGGCCCCGTACACCGTGTGCAACAGCTCCCTCTCGGAGTACG
  GAGTCCTGGGCTTTGAGCTGGGCTATGCCATGGCCAGCCCCAATGCCCTGGTCCTCTGGGAGGCCCAGTT
  TGGGGACTTCCACAACACGGCCCAGTGCATCATCGACCAGTTCATCAGCACCGGCCAGGCCAAGTGGGTG
  CGGCATAATGGCATTGTGCTGCTGCTGCCCCATGGCATGGAAGGCATGGGCCCAGAGCACTCGTCAGCGA
  GGCCCGAAAGGTTCCTGCAGATGAGCAATGATGACTCGGATGCCTACCCTGCATTCACCAAGGACTTCGA
  GGTGAGCCAGCTCTATGACTGCAACTGGATCGTGGTCAACTGCTCCACACCGGCCAACTACTTCCACGTG
  CTGCGCCGGCAGATCCTGCTGCCCTTCCGCAAGCCGCTGATTATCTTCACACCTAAATCTCTGCTGAGGC
  ACCCAGAGGCCAAGTCCAGCTTTGACCAAATGGTATCCGGGACCAGCTTCCAGCGGGTGATTCCTGAAGA
  TGGGGCCGCAGCACGGGCCCCTGAGCAGGTGCAGCGGCTCATCTTCTGCACGGGAAAGGTGTACTATGAC
  CTGGTGAAGGAGCGGAGCAGCCAGGACCTGGAGGAGAAAGTGGCCATCACGCGCCTGGAGCAGATCTCTC
  CATTCCCCTTCGACCTGATCAAGCAGGAGGCAGAGAAGTACCCAGGTGCGGAGCTGGCCTGGTGTCAGGA
  GGAGCACAAGAACATGGGCTACTATGACTACATCAGCCCACGCTTCATGACCATCCTGAGGCGCGCACGG
  CCCATATGGTATGTTGGCCGGGACCCAGCGGCTGCACCAGCCACAGGAAACAGGAACACTCACCTGGTGT
  CACTGAAGAAGTTTCTGGATACTGCCTTCAATCTCCAGGCCTTTGAGGGCAAGACATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227996 representing NM_001143996
  Red=Cloning site Green=Tags(s)
  
  MSQLRLLPSRLGVQAARLLAAHDVPVFGWRSRSSGPPATFPSSKGGGGSSYMEEMYFAWLENPQSVHKIR
  GHHVAQLDPLGILDADLDSFVPSDLITTIDKLAFYDLQEADLDKEFQLPTTTFIGGSENTLSLREIIRRL
  ENTYCQHIGLEFMFINDVEQCQWIRQKFETPGVMQFSSEEKRTLLARLVRSMRFEDFLARKWSSEKRFGL
  EGCEVMIPALKTIIDKSSEMGIENVILGMPHRGRLNVLANVIRKDLEQIFCQFDPKLEAADEGSGDVKYH
  LGMYHERINRVTNRNITLSLVANPSHLEAVDPVVQGKTKAEQFYRGDAQGKKVMSILVHGDAAFAGQGVV
  YETFHLSDLPSYTTNGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNADDPEAVIYVCSVA
  AEWRNTFNKDVVVDLVCYRRRGHNEMDEPMFTQPLMYKQIHRQVPVLKKYADKLIAEGTVTLQEFEEEIA
  KYDRICEEAYGRSKDKKILHIKHWLDSPWPGFFNVDGEPKSMTCPATGIPEDMLTHIGSVASSVPLEDFK
  IHTGLSRILRGRADMTKNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQEVDRRTC
  VPMNHLWPDQAPYTVCNSSLSEYGVLGFELGYAMASPNALVLWEAQFGDFHNTAQCIIDQFISTGQAKWV
  RHNGIVLLLPHGMEGMGPEHSSARPERFLQMSNDDSDAYPAFTKDFEVSQLYDCNWIVVNCSTPANYFHV
  LRRQILLPFRKPLIIFTPKSLLRHPEAKSSFDQMVSGTSFQRVIPEDGAAARAPEQVQRLIFCTGKVYYD
  LVKERSSQDLEEKVAITRLEQISPFPFDLIKQEAEKYPGAELAWCQEEHKNMGYYDYISPRFMTILRRAR
  PIWYVGRDPAAAPATGNRNTHLVSLKKFLDTAFNLQAFEGKTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143996
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143996.1, NP_001137468.1
• RefSeq ORF: 2862
• Locus ID: 55753
• Protein Pathways: Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways, Tryptophan metabolism
• MW: 108 kDa
• Gene Summary: The protein encoded by this gene is similar to oxoglutarate dehydrogenase (OGDH) of the OGDH complex, which degrades glucose and glutamate. This gene encodes several isoforms, including some that appear to localize to mitochondria. The encoded protein down-regulates the AKT signaling cascade and can suppress the growth of cervical cancer cells. [provided by RefSeq, Dec 2016]