TBXAS1 (NM_001166253) Human Tagged ORF Clone

CAT#: RC228471

TBXAS1 (Myc-DDK-tagged)-Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBXAS1
• Synonyms: BDPLT14; CYP5; CYP5A1; GHOSAL; THAS; TS; TXAS; TXS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228471 representing NM_001166253
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAGCCTTGGGGTTTCTAAAATTGGAAGTGAATGGCCCCATGGTGACGGTGGCCCTGTCAGTGG
  CTCTCTTGGCCCTCCTGAAATGGTACTCCACATCAGCATTCTCAAGACTGGAGAAGTTAGGCCTCAGACA
  TCCCAAGCCTTCTCCTTTCATTGGAAACTTGACATTTTTCCGCCAGGGTTTTTGGGAAAGCCAAATGGAG
  CTCAGAAAGCTGTATGGACCTCTGTGTGGGTACTATCTTGGTCGTCGGATGTTTATTGTTATTTCTGAGC
  CAGACATGATCAAGCAGGTGTTGGTTGAGAACTTCAGTAACTTTACCAACAGAATGGCGTCGGGTTTGGA
  GTTCAAGTCGGTAGCCGACAGCGTTCTGTTTTTACGTGACAAAAGATGGGAAGAGGTCAGAGGTGCCCTG
  ATGTCTGCTTTCAGTCCTGAAAAGCTGAACGAGCTTGGCCTTTTAATCATGCAAGAGAGAATAAAAGGTC
  ACATGGGTGGCCAGCAGGCTCCACAAAGAATTCCACCCACCCGTTTGTCGAAGCCGAGTGGTATCTATGT
  GAATCTCCATTATGCCACTCTACCTTTCTGTATGGTTCCCCTCATCAGCCAAGCCTGCGACCTTCTCCTG
  GCTCATTTAAAACGCTATGCGGAATCTGGGGACGCATTTGACATCCAGAGGTGCTACTGCAATTACACCA
  CAGATGTGGTTGCCAGCGTCGCCTTTGGCACCCCGGTGGACTCCTGGCAGGCCCCTGAGGATCCCTTTGT
  GAAACACTGCAAGCGTTTCTTCGAATTCTGCATCCCCAGACCTATCCTGGTTTTACTCTTATCATTTCCA
  TCCATAATGGTCCCACTGGCCCGGATTTTGCCCAATAAGAACCGAGACGAACTGAATGGCTTTTTTAACA
  AACTCATTAGGAATGTGATTGCCTTGCGGGACCAGCAAGCTGCCGAAGAGAGGCGGAGAGACTTCCTCCA
  AATGGTCCTGGATGCCCGACATTCTGCAAGTCCCATGGGCGTGCAAGACTTTGACATCGTCAGAGACGTT
  TTCTCCTCTACTGGGTGCAAGCCGAACCCTTCCCGGCAACACCAGCCCAGCCCTATGGCCAGGCCTTTGA
  CTGTGGATGAGATTGTGGGCCAGGCCTTCATCTTCCTCATCGCTGGCTATGAAATCATCACCAACACACT
  TTCTTTTGCCACCTACCTACTGGCCACCAACCCTGACTGCCAAGAGAAGCTTCTGAGAGAGGTAGACGTT
  TTTAAGGAGAAACACATGGCCCCTGAGTTCTGCAGCCTCGAGGAAGGCCTGCCCTATCTGGACATGGTGA
  TTGCAGAGACGCTGAGGATGTACCCGCCAGCTTTCAGATTCACACGGGAGGCAGCTCAGGACTGCGAGGT
  GCTGGGGCAGCGCATCCCCGCAGGCGCTGTGCTAGAGATGGCCGTGGGTGCCCTGCACCATGACCCTGAG
  CACTGGCCAAGCCCGGAGACCTTCAACCCTGAAAGGTTCACGGCTGAGGCCCGGCAGCAGCACCGGCCCT
  TCACGTACCTGCCCTTCGGGGCCGGCCCACGGAGCTGCCTCGGGGTGCGTCTAGGGCTGCTTGAGGTCAA
  GTTGACACTGCTCCACGTGCTGCACAAGTTCCGGTTCCAAGCCTGCCCTGAGACCCAGGTACCGCTGCAG
  CTAGAATCCAAATCTGCCCTAGGTCCAAAAAATGGTGTCTATATCAAGATCGTATCCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228471 representing NM_001166253
  Red=Cloning site Green=Tags(s)
  
  MMEALGFLKLEVNGPMVTVALSVALLALLKWYSTSAFSRLEKLGLRHPKPSPFIGNLTFFRQGFWESQME
  LRKLYGPLCGYYLGRRMFIVISEPDMIKQVLVENFSNFTNRMASGLEFKSVADSVLFLRDKRWEEVRGAL
  MSAFSPEKLNELGLLIMQERIKGHMGGQQAPQRIPPTRLSKPSGIYVNLHYATLPFCMVPLISQACDLLL
  AHLKRYAESGDAFDIQRCYCNYTTDVVASVAFGTPVDSWQAPEDPFVKHCKRFFEFCIPRPILVLLLSFP
  SIMVPLARILPNKNRDELNGFFNKLIRNVIALRDQQAAEERRRDFLQMVLDARHSASPMGVQDFDIVRDV
  FSSTGCKPNPSRQHQPSPMARPLTVDEIVGQAFIFLIAGYEIITNTLSFATYLLATNPDCQEKLLREVDV
  FKEKHMAPEFCSLEEGLPYLDMVIAETLRMYPPAFRFTREAAQDCEVLGQRIPAGAVLEMAVGALHHDPE
  HWPSPETFNPERFTAEARQQHRPFTYLPFGAGPRSCLGVRLGLLEVKLTLLHVLHKFRFQACPETQVPLQ
  LESKSALGPKNGVYIKIVSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166253
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166253.1, NP_001159725.1
• RefSeq ORF: 1740 bp
• Locus ID: 6916
• Cytogenetics: 7q34
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• MW: 65.6 kDa
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'