TKTL1 (NM_001145933) Human Tagged ORF Clone

CAT#: RC228477

TKTL1 (Myc-DDK-tagged)-Human transketolase-like 1 (TKTL1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY431505) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228477 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TKTL1
• Synonyms: TKR; TKT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228477 representing NM_001145933
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGCTGAGGCGAGGGCTGAGTTCCCGGAGGAGGCCAGACCTGACAGGGGCACCTTGCAGGTGT
  TGCAAGATATGGCCAGCCGCTTGCGAATCCATTCCATCAGGGCCACATGCTCCACGAGCTCCGGCAGTTC
  TTCTGAGATCATGTCTGTGCTGTTCTTCTACATCATGAGGTACAAGCAGTCAGATCCAGAGAATCCGGAC
  AACGACCGATTTGTCCTCGCAAAGAGACTGTCGTTTGTGGATGTGGCAACAGGATGGCTCGGACAAGGAC
  TGGGAGTTGCATGTGGAATGGCATATACTGGCAAGTACTTCGACAGGGCCAGCTACCGGGTGTTCTGCCT
  CATGAGTGATGGCGAGTCCTCAGAAGGCTCTGTCTGGGAGGCAATGGCCTTTGCTTCCTACTACAGTCTG
  GACAATCTTGTGGCAATCTTTGATGTGAACCGCCTGGGACACAGTGGTGCATTGCCCGCCGAGCACTGCA
  TAAACATCTATCAGAGGCGCTGCGAAGCCTTTGGGTGGAACACTTATGTGGTGGACGGCCGGGACGTGGA
  GGCACTGTGCCAGGTATTCTGGCAGGCTTCTCAGGTGAAGCACAAGCCCACTGCTGTGGTGGCCAAGACC
  TTCAAGGGCCGGGGCACCCCAAGTATTGAGGATGCAGAAAGTTGGCATGCAAAGCCAATGCCGAGAGAAA
  GAGCAGATGCCATTATCAAATTAATTGAGAGCCAGATACAGACCAGCAGGAATCTTGACCCACAGCCCCC
  CATTGAGGACTCACCTGAAGTCAACATCACAGATGTAAGGATGACCTCTCCACCTGATTACAGAGTTGGT
  GACAAGATAGCTACTCGGAAAGCATGCGGTCTGGCTCTGGCTAAGCTGGGCTACGCGAACAACAGAGTCG
  TTGTGCTGGATGGTGACACCAGGTACTCTACTTTCTCTGAGATATTCAACAAGGAGTACCCTGAGCGCTT
  CATCGAGTGCTTTATGGCTGAACAAAACATGGTGAGCGTGGCTCTGGGCTGTGCCTCCCGTGGACGGACC
  ATTGCTTTTGCTAGCACCTTTGCTGCCTTTCTGACTCGAGCATTTGATCACATCCGGATAGGAGGCCTCG
  CTGAGAGCAACATCAACATTATTGGTTCCCACTGTGGGGTATCTGTTGGTGACGATGGTGCTTCCCAGAT
  GGCCCTGGAGGATATAGCCATGTTCCGAACCATTCCCAAGTGCACGATCTTCTACCCAACTGATGCCGTC
  TCCACGGAGCATGCTGTTGCTCTGGCAGCCAATGCCAAGGGGATGTGCTTCATTCGGACCACCCGACCAG
  AAACTATGGTTATTTACACCCCACAAGAACGCTTTGAGATCGGACAGGCCAAGGTCCTCCGCCACTGTGT
  CAGTGACAAGGTCACAGTTATTGGAGCTGGAATTACTGTGTATGAAGCCTTAGCAGCTGCTGATGAGCTT
  TCGAAACAAGATATTTTTATCCGTGTCATCGACCTGTTTACCATTAAACCTCTGGATGTCGCCACCATCG
  TCTCCAGTGCAAAAGCCACAGAGGGCCGGATCATTACAGTGGAGGATCACTACCCGCAAGGTGGCATCGG
  GGAAGCTGTCTGCGCAGCCGTCTCCATGGATCCTGACATTCAGGTTCATTCGCTGGCAGTGTCGGGAGTG
  CCCCAGAGTGGGAAGTCCGAGGAATTGCTGGATATGTATGGAATTAGTGCCAGACATATCATAGTGGCCG
  TGAAATGCATGTTGCTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228477 representing NM_001145933
  Red=Cloning site Green=Tags(s)
  
  MADAEARAEFPEEARPDRGTLQVLQDMASRLRIHSIRATCSTSSGSSSEIMSVLFFYIMRYKQSDPENPD
  NDRFVLAKRLSFVDVATGWLGQGLGVACGMAYTGKYFDRASYRVFCLMSDGESSEGSVWEAMAFASYYSL
  DNLVAIFDVNRLGHSGALPAEHCINIYQRRCEAFGWNTYVVDGRDVEALCQVFWQASQVKHKPTAVVAKT
  FKGRGTPSIEDAESWHAKPMPRERADAIIKLIESQIQTSRNLDPQPPIEDSPEVNITDVRMTSPPDYRVG
  DKIATRKACGLALAKLGYANNRVVVLDGDTRYSTFSEIFNKEYPERFIECFMAEQNMVSVALGCASRGRT
  IAFASTFAAFLTRAFDHIRIGGLAESNINIIGSHCGVSVGDDGASQMALEDIAMFRTIPKCTIFYPTDAV
  STEHAVALAANAKGMCFIRTTRPETMVIYTPQERFEIGQAKVLRHCVSDKVTVIGAGITVYEALAAADEL
  SKQDIFIRVIDLFTIKPLDVATIVSSAKATEGRIITVEDHYPQGGIGEAVCAAVSMDPDIQVHSLAVSGV
  PQSGKSEELLDMYGISARHIIVAVKCMLLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145933
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145933.1, NP_001139405.1
• RefSeq ORF: 1773
• Locus ID: 8277
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• MW: 64.5 kDa
• Gene Summary: The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolytic pathway. Variations in this gene may be the cause of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]