NEK11 (NM_001146003) Human Tagged ORF Clone

CAT#: RC228483

NEK11 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY431511) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228483 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEK11
• Synonyms: FLJ23495
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228483 representing NM_001146003
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAATTCCAAGAGGCAGCTAAGTGTGTGAGTGGATCAACAGCCATTTCCACTTATCCAAAGACCT
  TGATTGCAAGAAGATACGTGCTTCAACAAAAACTTGGCAGTGGAAGTTTTGGAACTGTCTATCTGGTTTC
  AGACAAGAAAGCCAAACGAGGAGAGGAATTAAAGGTACTTAAGGAAATATCTGTTGGAGAACTAAATCCA
  AATGAAACTGTACAGGCCAATTTGGAAGCCCAACTCCTCTCCAAGCTGGACCACCCAGCCATTGTCAAGT
  TCCATGCAAGTTTTGTGGAGCAAGATAATTTCTGCATTATCACGGAGTACTGTGAGGGCCGAGATCTGGA
  CGATAAAATTCAGGAATATAAACAAGCTGGAAAAATCTTTCCAGAAAATCAAATAATAGAATGGTTTATC
  CAGCTGCTGCTGGGAGTTGACTACATGCATGAGAGGAGGATACTTCATCGAGACTTAAAGTCAAAGAATG
  TATTTCTGAAAAATAATCTCCTTAAAATTGGAGATTTTGGAGTTTCTCGACTTCTAATGGGATCCTGTGA
  CCTGGCCACAACTTTAACTGGAACTCCCCATTATATGAGTCCTGAGGCTCTGAAACACCAAGGCTATGAC
  ACAAAGTCGGACATCTGGTCACTGGCATGCATTTTGTATGAGATGTGCTGCATGAATCATGCATTCGCTG
  GCTCCAATTTCTTATCCATTGTTTTAAAAATTGTTGAAGGTGACACACCTTCTCTCCCTGAGAGATATCC
  AAAAGAACTAAATGCCATCATGGAAAGCATGTTGAACAAGAATCCTTCATTAAGACCATCTGCTATCGAA
  ATTTTAAAAATCCCTTACCTTGATGAGCAGCTACAGAACCTAATGTGTAGATATTCAGAAATGACTCTGG
  AAGACAAAAATTTGGATTGTCAGAAGGAGGCTGCTCATATAATTAATGCCATGCAAAAAAGGATCCACCT
  GCAGACTCTGAGGGCACTGTCAGAAGTACAGAAAATGACGCCAAGAGAAAGGATGCGGCTGAGGAAGCTC
  CAGGCGGCTGATGAGAAAGCCAGGAAGCTGAAAAAGATTGTGGAAGAAAAATATGAAGAAAATAGCAAAC
  GAATGCAAGAATTGAGATCTCGGAACTTTCAGCAGCTGAGTGTTGATGTACTCCATGAAAAAACACATTT
  AAAAGGAATGGAAGAAAAGGAGGAGCAACCTGAGGGAAGACTTTCTTGTTCACCCCAGGACGAGGATGAA
  GAGAGGTGGCAAGGCAGGGAAGAGGAATCTGATGAACCAACTTTAGAGAACCTGCCTGAGTCTCAGCCTA
  TTCCTTCCATGGACCTCCACGAACTTGAATCAATTGTAGAGGATGCCACATCTGACCTTGGATACCATGA
  GATCCCAGAAGACCCACTTGTGGCTGAAGAGTACTACGCTGATGCATTTGATTCCTATTGTGAAGAGAGT
  GATGAGGAGGAAGAAGAAATAGCGTTAGAAAGACCAGAGAAAGAAATCAGGAATGAGGGATCCCAGCCTG
  CTTACAGAACAAACCAACAGGACAGTGATATCGAAGCGTTGGCCAGGTGTTTGGAAAATGTCCTGGGTTG
  CACTTCTCTAGATCAGCCATGCAGAAGCTGGGGACAGAAGTATTTGAAGAGGTCTATAATTACCTCAAGA
  GAGCAAGGCATCAGAATGCTAGCGAAGCAGAGATCCGCGAGTGTTTGGAAAAAGTGGTGCCTCAAGCCAG
  CGACTGTTTTGAAGTGGACCAGCTCCTGTACTTTGAAGAGCAGTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228483 representing NM_001146003
  Red=Cloning site Green=Tags(s)
  
  MLKFQEAAKCVSGSTAISTYPKTLIARRYVLQQKLGSGSFGTVYLVSDKKAKRGEELKVLKEISVGELNP
  NETVQANLEAQLLSKLDHPAIVKFHASFVEQDNFCIITEYCEGRDLDDKIQEYKQAGKIFPENQIIEWFI
  QLLLGVDYMHERRILHRDLKSKNVFLKNNLLKIGDFGVSRLLMGSCDLATTLTGTPHYMSPEALKHQGYD
  TKSDIWSLACILYEMCCMNHAFAGSNFLSIVLKIVEGDTPSLPERYPKELNAIMESMLNKNPSLRPSAIE
  ILKIPYLDEQLQNLMCRYSEMTLEDKNLDCQKEAAHIINAMQKRIHLQTLRALSEVQKMTPRERMRLRKL
  QAADEKARKLKKIVEEKYEENSKRMQELRSRNFQQLSVDVLHEKTHLKGMEEKEEQPEGRLSCSPQDEDE
  ERWQGREEESDEPTLENLPESQPIPSMDLHELESIVEDATSDLGYHEIPEDPLVAEEYYADAFDSYCEES
  DEEEEEIALERPEKEIRNEGSQPAYRTNQQDSDIEALARCLENVLGCTSLDQPCRSWGQKYLKRSIITSR
  EQGIRMLAKQRSASVWKKWCLKPATVLKWTSSCTLKSSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146003
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146003.1, NP_001139475.1
• RefSeq ORF: 1800
• Locus ID: 79858
• Protein Families: Druggable Genome, Protein Kinase
• MW: 68.7 kDa
• Gene Summary: This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]