Acinus (ACIN1) (NM_001164816) Human Tagged ORF Clone

CAT#: RC228502

ACIN1 (Myc-DDK-tagged)-Human apoptotic chromatin condensation inducer 1 (ACIN1), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY431530) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228502 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACIN1
• Synonyms: ACINUS; ACN; fSAP152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228502 representing NM_001164816
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCGGCTGATCGCTGCCGCTCCGCCAATACAATAGAGCCAGCCACTACCAGCAGCCTGGCCCTCT
  TCCTCCTTCTCCAGAGAGACCAATCCAGCCGAACTCGGGGTTTGCCTGAGGAGAAGGAGGAAGTGACCAT
  GGACACAAGTGAAAACAGACCTGAAAATGATGTTCCAGAACCTCCCATGCCTATTGCAGACCAAGTCAGC
  AATGATGACCGCCCGGAGGGCAGTGTTGAAGATGAGGAGAAGAAAGAGAGCTCGCTGCCCAAATCATTCA
  AGAGGAAGATCTCCGTTGTCTCAGCTACCAAGGGGGTGCCAGCTGGAAACAGTGACACAGAGGGGGGCCA
  GCCTGGTCGGAAACGACGCTGGGGAGCCAGCACAGCCACCACACAGAAGAAACCTTCCATCAGTATCACC
  ACTGAATCACTAAAGAGCCTCATCCCCGACATCAAACCCCTGGCGGGGCAGGAGGCTGTTGTGGATCTTC
  ATGCTGATGACTCTCGCATCTCTGAGGATGAGACAGAGCGTAATGGCGATGATGGGACCCATGACAAGGG
  GCTGAAAATATGCCGGACAGTCACTCAGGTAGTACCTGCAGAGGGCCAGGAGAATGGGCAGAGGGAAGAA
  GAGGAAGAAGAGAAGGAACCTGAAGCAGAACCTCCTGTACCTCCCCAGGTGTCAGTAGAGGTGGCCTTGC
  CCCCACCTGCAGAGCATGAAGTAAAGAAAGTGACTTTAGGAGATACCTTAACTCGACGTTCCATTAGCCA
  GCAGAAGTCCGGAGTTTCCATTACCATTGATGACCCAGTCCGAACTGCCCAGGTGCCCTCCCCACCCCGG
  GGCAAGATTAGCAACATTGTCCATATCTCCAATTTGGTCCGTCCTTTCACTTTAGGCCAGCTAAAGGAGT
  TGTTGGGGCGCACAGGAACCTTGGTGGAAGAGGCCTTCTGGATTGACAAGATCAAATCTCATTGCTTTGT
  AACGTACTCAACAGTAGAGGAAGCTGTTGCCACCCGCACAGCTCTGCACGGGGTCAAATGGCCCCAGTCC
  AATCCCAAATTCCTTTGTGCTGACTATGCCGAGCAAGATGAGCTGGATTATCACCGAGGCCTCTTGGTGG
  ACCGTCCCTCTGAAACTAAGACAGAGGAGCAGGGAATACCACGGCCCCTGCACCCCCCACCCCCACCCCC
  GGTCCAGCCACCACAGCACCCCCGGGCAGAGCAGCGGGAGCAGGAACGGGCAGTGCGGGAACAGTGGGCA
  GAACGGGAACGGGAAATGGAGCGGCGGGAGCGGACTCGATCAGAGCGTGAATGGGATCGGGACAAAGTTC
  GAGAAGGGCCCCGTTCCCGATCAAGGTCCCGTGACCGCCGCCGCAAGGAACGTGCGAAGTCTAAAGAAAA
  GAAGAGTGAGAAGAAAGAGAAAGCCCAGGAGGAACCACCTGCCAAGCTGCTGGATGACCTTTTCCGAAAG
  ACCAAGGCAGCTCCCTGCATCTATTGGCTCCCACTGACTGACAGCCAGATCGTTCAGAAAGAGGCAGAGC
  GGGCCGAACGGGCCAAGGAGCGGGAGAAGCGGCGAAAGGAGCAAGAAGAAGAAGAGCAAAAGGAGCGGGA
  GAAGGAAGCCGAGCGGGAACGGAACCGACAGCTGGAGCGAGAGAAACGTCGGGAGCACAGTCGGGAGAGG
  GACAGGGAGAGAGAGAGAGAAAGGGAGCGGGACAGGGGGGACCGAGATCGGGATAGGGAAAGGGACCGAG
  AACGAGGCAGGGAAAGGGATCGCAGGGACACCAAGCGCCACAGCAGAAGCCGGAGTCGGAGCACACCTGT
  GCGGGACCGGGGTGGGCGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228502 representing NM_001164816
  Red=Cloning site Green=Tags(s)
  
  MSPADRCRSANTIEPATTSSLALFLLLQRDQSSRTRGLPEEKEEVTMDTSENRPENDVPEPPMPIADQVS
  NDDRPEGSVEDEEKKESSLPKSFKRKISVVSATKGVPAGNSDTEGGQPGRKRRWGASTATTQKKPSISIT
  TESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVTQVVPAEGQENGQREE
  EEEEKEPEAEPPVPPQVSVEVALPPPAEHEVKKVTLGDTLTRRSISQQKSGVSITIDDPVRTAQVPSPPR
  GKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQS
  NPKFLCADYAEQDELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWA
  EREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRK
  TKAAPCIYWLPLTDSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRER
  DRERERERERDRGDRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164816
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164816.1, NP_001158288.1
• RefSeq ORF: 1845
• Locus ID: 22985
• Protein Pathways: Spliceosome
• MW: 70.8 kDa
• Gene Summary: Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. It may thus be involved in mRNA metabolism associated with splicing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]