HDAC10 (NM_001159286) Human Tagged ORF Clone

CAT#: RC228518

HDAC10 (Myc-DDK-tagged)-Human histone deacetylase 10 (HDAC10), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY431546) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228518 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HDAC10
• Synonyms: HD10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228518 representing NM_001159286
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCGCGCTTGTGTACCATGAGGACATGACGGCCACCCGGCTGCTCTGGGACGACCCCGAGTGCG
  AGATCGAGCGTCCTGAGCGCCTGACCGCAGCCCTGGATCGCCTGCGGCAGCGCGGCCTGGAACAGAGGTG
  TCTGCGGTTGTCAGCCCGCGAGGCCTCGGAAGAGGAGCTGGGCCTGGTGCACAGCCCAGAGTATGTATCC
  CTGGTCAGGGAGACCCAGGTCCTAGGCAAGGAGGAGCTGCAGGCGCTGTCCGGACAGTTCGACGCCATCT
  ACTTCCACCCGAGTACCTTTCACTGCGCGCGGCTGGCCGCAGGGGCTGGACTGCAGCTGGTGGACGCTGT
  GCTCACTGGAGCTGTGCAAAATGGGCTTGCCCTGGTGAGGCCTCCCGGGCACCATGGCCAGAGGGCGGCT
  GCCAACGGGTTCTGTGTGTTCAACAACGTGGCCATAGCAGCTGCACATGCCAAGCAGAAACACGGGCTAC
  ACAGGATCCTCGTCGTGGACTGGGATGTGCACCATGGCCAGGGGATCCAGTATCTCTTTGAGGATGACCC
  CAGCGTCCTTTACTTCTCCTGGCACCGCTATGAGCATGGGCGCTTCTGGCCTTTCCTGCGAGAGTCAGAT
  GCAGACGCAGTGGGGCGGGGACAGGGCCTCGGCTTCACTGTCAACCTGCCCTGGAACCAGGTTGGGATGG
  GAAACGCTGACTACGTGGCTGCCTTCCTGCACCTGCTGCTCCCACTGGCCTTTGAGGGGCAAATGCAGGC
  CACGCCAGAGTGCTTCGCCCACCTCACACAGCTGCTGCAGGTGCTGGCCGGCGGCCGGGTCTGTGCCGTG
  CTGGAGGGCGGCTACCACCTGGAGTCACTGGCGGAGTCAGTGTGCATGACAGTACAGACGCTGCTGGGTG
  ACCCGGCCCCACCCCTGTCAGGGCCAATGGCGCCATGTCAGAGTGCCCTAGAGTCCATCCAGAGTGCCCG
  TGCTGCCCAGGCCCCGCACTGGAAGAGCCTCCAGCAGCAAGATGTGACCGCTGTGCCGATGAGCCCCAGC
  AGCCACTCCCCAGAGGGGAGGCCTCCACCTCTGCTGCCTGGGGGTCCAGTGTGTAAGGCAGCTGCATCTG
  CACCGAGCTCCCTCCTGGACCAGCCGTGCCTCTGCCCCGCACCCTCTGTCCGCACCGCTGTTGCCCTGAC
  AACGCCGGATATCACATTGGTTCTGCCCCCTGACGTCATCCAACAGGAAGCGTCAGCCCTGAGGGAGGAG
  ACAGAAGCCTGGGCCAGGCCACACGAGTCCCTGGCCCGGGAGGAGGCCCTCACTGCACTTGGGAAGCTCC
  TGTACCTCTTAGATGGGATGCTGGATGGGCAGGTGAACAGTGGTATAGCAGCCACTCCAGCCTCTGCTGC
  AGCAGCCACCCTGGATGTGGCTGTTCGGAGAGGCCTGTCCCACGGAGCCCAGAGGCTGCTGTGCGTGGCC
  CTGGGACAGCTGGACCGGCCTCCAGACCTCGCCCATGACGGGAGGAGTCTGTGGCTGAACATCAGGGGCA
  AGGAGGCGGCTGCCCTATCCATGTTCCATGTCTCCACGCCACTGCCAGTGATGACCGGTGGTTTCCTGAG
  CTGCATCTTGGGCTTGGTGCTGCCCCTGGCCTATGGCTTCCAGCCTGACCTGGTGCTGGTGGCGCTGGGG
  CCTGGCCATGGCCTGCAGGGCCCCCACGCTGCACTCCTGGCTGCAATGCTTCGGGGGCTGGCAGGGGGCC
  GAGTCCTGGCCCTCCTGGAGGAGAACTCCACACCCCAGCTAGCAGGGATCCTGGCCCGGGTGCTGAATGG
  AGAGGCACCTCCTAGCCTAGGCCCTTCCTCTGTGGCCTCCCCAGAGGACGTCCAGGCCCTGATGTACCTG
  AGAGGGCAGCTGGAGCCTCAGTGGAAGATGTTGCAGTGCCATCCTCACCTGGTGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228518 representing NM_001159286
  Red=Cloning site Green=Tags(s)
  
  MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVS
  LVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAA
  ANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESD
  ADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEGQMQATPECFAHLTQLLQVLAGGRVCAV
  LEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQAPHWKSLQQQDVTAVPMSPS
  SHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREE
  TEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAATLDVAVRRGLSHGAQRLLCVA
  LGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCILGLVLPLAYGFQPDLVLVALG
  PGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYL
  RGQLEPQWKMLQCHPHLVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159286
• ORF Size: 1947 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159286.1, NP_001152758.1
• RefSeq ORF: 1950
• Locus ID: 83933
• Protein Families: Druggable Genome, Transcription Factors
• MW: 69.2 kDa
• Gene Summary: The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]