SLC7A2 (NM_001164771) Human Tagged ORF Clone

CAT#: RC228543

SLC7A2 (Myc-DDK-tagged)-Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 (SLC7A2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC7A2
• Synonyms: ATRC2; CAT2; HCAT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228543 representing NM_001164771
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATAGAAACAAGTGGTTATAACTCAGACAAACTAATTTGTCGAGGGTTTATTGGAACACCTGCCC
  CACCGGTTTGCGACAGCAAGTTTCTCCTGTCGCCTTCGTCAGACGTCAGAATGATTCCTTGCAGAGCCGC
  GCTGACCTTTGCCCGATGTCTGATCCGGAGAAAAATCGTGACCCTGGACAGTCTAGAAGACACCAAATTA
  TGCCGCTGCTTATCCACCATGGACCTCATTGCCCTGGGCGTTGGAAGCACCCTTGGGGCCGGGGTTTATG
  TCCTCGCTGGGGAGGTGGCCAAGGCAGACTCGGGCCCCAGCATCGTGGTGTCCTTCCTCATTGCTGCCCT
  GGCTTCAGTGATGGCTGGCCTCTGCTATGCCGAATTTGGGGCCCGTGTTCCCAAGACGGGGTCTGCATAT
  TTGTACACCTACGTGACTGTCGGAGAGCTGTGGGCCTTCATCACTGGCTGGAATCTCATTTTATCGTATG
  TGATAGGTACATCAAGTGTTGCAAGAGCCTGGAGTGGCACCTTTGATGAACTTCTTAGCAAACAGATTGG
  TCAGTTTTTGAGGACATACTTCAGAATGAATTACACTGGTCTTGCAGAATATCCCGATTTTTTTGCTGTG
  TGCCTTATATTACTTCTAGCAGGTCTTTTGTCTTTTGGAGTAAAAGAGTCTGCTTGGGTGAATAAAGTCT
  TCACAGCTGTTAATATTCTCGTCCTTCTGTTTGTGATGGTTGCTGGGTTTGTGAAAGGAAATGTGGCAAA
  CTGGAAGATTAGTGAAGAGTTTCTCAAAAATATATCAGCAAGTGCCAGAGAGCCACCTTCTGAAAACGGA
  ACAAGTATCTATGGGGCTGGTGGCTTTATGCCTTATGGCTTTACGGGAACGTTGGCTGGTGCTGCAACTT
  GCTTTTATGCCTTTGTGGGATTTGACTGCATTGCAACAACTGGTGAAGAAGTTCGGAATCCCCAGAAAGC
  TATTCCCATTGGAATTGTGACGTCTTTGCTTGTTTGCTTTATGGCCTATTTTGGGGTCTCTGCAGCTTTA
  ACACTTATGATGCCGTACTACCTCCTCGATGAAAAAAGCCCCCTTCCTGTAGCGTTTGAATATGTGGGAT
  GGGGTCCTGCCAAATATGTCGTCGCAGCTGGTTCTCTCTGCGCCTTGTCAACAAGTCTTCTTGGATCCAT
  TTTCCCAATGCCTCGTGTAATCTATGCTATGGCGGAGGATGGGTTGCTTTTCAAATGTCTAGCTCAAATC
  AATTCCAAAACGAAGACACCAATAATTGCTACTTTATCATCGGGTGCAGTGGCAGCTTTGATGGCCTTTC
  TGTTTGACCTGAAGGCGCTTGTGGACATGATGTCCATTGGCACACTCATGGCCTACTCTCTGGTGGCAGC
  CTGTGTTCTCATCCTCAGGTACCAGCCTGGCTTATCTTACGACCAGCCCAAATGTTCTCCTGAGAAAGAT
  GGTCTGGGATCGTCTCCCAGGGTAACCTCGAAGAGTGAGTCCCAGGTCACCATGCTGCAGAGACAGGGCT
  TCAGCATGCGGACCCTCTTCTGCCCCTCCCTTCTGCCAACACAGCAGTCAGCTTCTCTCGTGAGCTTTCT
  GGTAGGATTCCTAGCTTTCCTCGTGTTGGGCCTGAGTGTCTTGACCACTTACGGAGTTCATGCCATCACC
  AGGCTGGAGGCCTGGAGCCTCGCTCTCCTCGCGCTGTTTCTTGTTCTCTTCGTTGCCATCGTTCTCACCA
  TCTGGAGGCAGCCCCAGAATCAGCAAAAAGTAGCCTTCATGGTTCCATTCTTACCATTTTTGCCAGCGTT
  CAGCATCTTGGTGAACATTTACTTGATGGTCCAGTTAAGTGCAGACACTTGGGTCAGATTCAGCATTTGG
  ATGGCAATTGGCTTCCTGATTTACTTTTCTTATGGCATTAGACACAGCCTGGAGGGTCATCTGAGAGATG
  AAAACAATGAAGAAGATGCTTATCCAGACAACGTTCATGCAGCAGCAGAAGAAAAATCTGCCATTCAAGC
  AAATGACCATCACCCAAGAAATCTCAGTTCACCTTTCATATTCCATGAAAAGACAAGTGAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228543 representing NM_001164771
  Red=Cloning site Green=Tags(s)
  
  MKIETSGYNSDKLICRGFIGTPAPPVCDSKFLLSPSSDVRMIPCRAALTFARCLIRRKIVTLDSLEDTKL
  CRCLSTMDLIALGVGSTLGAGVYVLAGEVAKADSGPSIVVSFLIAALASVMAGLCYAEFGARVPKTGSAY
  LYTYVTVGELWAFITGWNLILSYVIGTSSVARAWSGTFDELLSKQIGQFLRTYFRMNYTGLAEYPDFFAV
  CLILLLAGLLSFGVKESAWVNKVFTAVNILVLLFVMVAGFVKGNVANWKISEEFLKNISASAREPPSENG
  TSIYGAGGFMPYGFTGTLAGAATCFYAFVGFDCIATTGEEVRNPQKAIPIGIVTSLLVCFMAYFGVSAAL
  TLMMPYYLLDEKSPLPVAFEYVGWGPAKYVVAAGSLCALSTSLLGSIFPMPRVIYAMAEDGLLFKCLAQI
  NSKTKTPIIATLSSGAVAALMAFLFDLKALVDMMSIGTLMAYSLVAACVLILRYQPGLSYDQPKCSPEKD
  GLGSSPRVTSKSESQVTMLQRQGFSMRTLFCPSLLPTQQSASLVSFLVGFLAFLVLGLSVLTTYGVHAIT
  RLEAWSLALLALFLVLFVAIVLTIWRQPQNQQKVAFMVPFLPFLPAFSILVNIYLMVQLSADTWVRFSIW
  MAIGFLIYFSYGIRHSLEGHLRDENNEEDAYPDNVHAAAEEKSAIQANDHHPRNLSSPFIFHEKTSEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164771
• ORF Size: 2094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164771.1, NP_001158243.1
• RefSeq ORF: 2097 bp
• Locus ID: 6542
• Cytogenetics: 8p22
• Protein Families: Druggable Genome, Transmembrane
• MW: 75.8 kDa
• Gene Summary: 'The protein encoded by this gene is a cationic amino acid transporter and a member of the APC (amino acid-polyamine-organocation) family of transporters. The encoded membrane protein is responsible for the cellular uptake of arginine, lysine and ornithine. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'