epithelial Sodium Channel alpha (SCNN1A) (NM_001159576) Human Tagged ORF Clone

CAT#: RC228555

SCNN1A (Myc-DDK-tagged)-Human sodium channel, nonvoltage-gated 1 alpha (SCNN1A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCNN1A
• Synonyms: BESC2; ENaCa; ENaCalpha; LIDLS3; SCNEA; SCNN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228555 representing NM_001159576
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATGGCCAGGGGCAGCCTCACTCGGGTTCCAGGGGTGATGGGAGAGGGCACTCAGGGCCCAGAGC
  TCAGCCTTGACCCTGACCCTTGCTCTCCCCAATCCACTCCGGGGCTCATGAAGGGGAACAAGCTGGAGGA
  GCAGGACCCTAGACCTCTGCAGCCCATACCAGGTCTCATGGAGGGGAACAAGCTGGAGGAGCAGGACTCT
  AGCCCTCCACAGTCCACTCCAGGGCTCATGAAGGGGAACAAGCGTGAGGAGCAGGGGCTGGGCCCCGAAC
  CTGCGGCGCCCCAGCAGCCCACGGCGGAGGAGGAGGCCCTGATCGAGTTCCACCGCTCCTACCGAGAGCT
  CTTCGAGTTCTTCTGCAACAACACCACCATCCACGGCGCCATCCGCCTGGTGTGCTCCCAGCACAACCGC
  ATGAAGACGGCCTTCTGGGCAGTGCTGTGGCTCTGCACCTTTGGCATGATGTACTGGCAATTCGGCCTGC
  TTTTCGGAGAGTACTTCAGCTACCCCGTCAGCCTCAACATCAACCTCAACTCGGACAAGCTCGTCTTCCC
  CGCAGTGACCATCTGCACCCTCAATCCCTACAGGTACCCGGAAATTAAAGAGGAGCTGGAGGAGCTGGAC
  CGCATCACAGAGCAGACGCTCTTTGACCTGTACAAATACAGCTCCTTCACCACTCTCGTGGCCGGCTCCC
  GCAGCCGTCGCGACCTGCGGGGGACTCTGCCGCACCCCTTGCAGCGCCTGAGGGTCCCGCCCCCGCCTCA
  CGGGGCCCGTCGAGCCCGTAGCGTGGCCTCCAGCTTGCGGGACAACAACCCCCAGGTGGACTGGAAGGAC
  TGGAAGATCGGCTTCCAGCTGTGCAACCAGAACAAATCGGACTGCTTCTACCAGACATACTCATCAGGGG
  TGGATGCGGTGAGGGAGTGGTACCGCTTCCACTACATCAACATCCTGTCGAGGCTGCCAGAGACTCTGCC
  ATCCCTGGAGGAGGACACGCTGGGCAACTTCATCTTCGCCTGCCGCTTCAACCAGGTCTCCTGCAACCAG
  GCGAATTACTCTCACTTCCACCACCCGATGTATGGAAACTGCTATACTTTCAATGACAAGAACAACTCCA
  ACCTCTGGATGTCTTCCATGCCTGGAATCAACAACGGTCTGTCCCTGATGCTGCGCGCAGAGCAGAATGA
  CTTCATTCCCCTGCTGTCCACAGTGACTGGGGCCCGGGTAATGGTGCACGGGCAGGATGAACCTGCCTTT
  ATGGATGATGGTGGCTTTAACTTGCGGCCTGGCGTGGAGACCTCCATCAGCATGAGGAAGGAAACCCTGG
  ACAGACTTGGGGGCGATTATGGCGACTGCACCAAGAATGGCAGTGATGTTCCTGTTGAGAACCTTTACCC
  TTCAAAGTACACACAGCAGGTGTGTATTCACTCCTGCTTCCAGGAGAGCATGATCAAGGAGTGTGGCTGT
  GCCTACATCTTCTATCCGCGGCCCCAGAACGTGGAGTACTGTGACTACAGAAAGCACAGTTCCTGGGGGT
  ACTGCTACTATAAGCTCCAGGTTGACTTCTCCTCAGACCACCTGGGCTGTTTCACCAAGTGCCGGAAGCC
  ATGCAGCGTGACCAGCTACCAGCTCTCTGCTGGTTACTCACGATGGCCCTCGGTGACATCCCAGGAATGG
  GTCTTCCAGATGCTATCGCGACAGAACAATTACACCGTCAACAACAAGAGAAATGGAGTGGCCAAAGTCA
  ACATCTTCTTCAAGGAGCTGAACTACAAAACCAATTCTGAGTCTCCCTCTGTCACGATGGTCACCCTCCT
  GTCCAACCTGGGCAGCCAGTGGAGCCTGTGGTTCGGCTCCTCGGTGTTGTCTGTGGTGGAGATGGCTGAG
  CTCGTCTTTGACCTGCTGGTCATCATGTTCCTCATGCTGCTCCGAAGGTTCCGAAGCCGATACTGGTCTC
  CAGGCCGAGGGGGCAGGGGTGCTCAGGAGGTAGCCTCCACCCTGGCATCCTCCCCTCCTTCCCACTTCTG
  CCCCCACCCCATGTCTCTGTCCTTGTCCCAGCCAGGCCCTGCTCCCTCTCCAGCCTTGACAGCCCCTCCC
  CCTGCCTATGCCACCCTGGGCCCCCGCCCATCTCCAGGGGGCTCTGCAGGGGCCAGTTCCTCCACCTGTC
  CTCTGGGGGGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228555 representing NM_001159576
  Red=Cloning site Green=Tags(s)
  
  MGMARGSLTRVPGVMGEGTQGPELSLDPDPCSPQSTPGLMKGNKLEEQDPRPLQPIPGLMEGNKLEEQDS
  SPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNR
  MKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELD
  RITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKD
  WKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQ
  ANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAF
  MDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGC
  AYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEW
  VFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAE
  LVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPP
  PAYATLGPRPSPGGSAGASSSTCPLGGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159576
• ORF Size: 2184 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159576.1, NP_001153048.1
• RefSeq ORF: 2187 bp
• Locus ID: 6337
• Cytogenetics: 12p13.31
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Taste transduction
• MW: 81.7 kDa
• Gene Summary: 'Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]'