DHX16 (NM_001164239) Human Tagged ORF Clone

CAT#: RC228627

DHX16 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 16 (DHX16), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DHX16
• Synonyms: DBP2; DDX16; PRO2014; Prp2; PRP8; PRPF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228627 representing NM_001164239
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAAGATCCTAGAGGTCCCAAGGTACCACGAAAGGCAGTGGTAGAAAAGCCAGCTCGGGCAGCAG
  AGCGAGAGGCCCGGGCCCTGCTGGAGAAGAACCGATCTTATAGGTTACTGGAAGACAGTGAAGAGAGCAG
  TGAGGAGACTGTGAGTAGGGCTGGAAGCAGCCTCCAGAAGAAACGTAAAAAGCGGAAACACCTCAGGAAG
  AAGCGTGAGGAAGAAGAGGAGGAAGAGGCTTCTGAGAAAGGGAAGAAGAAAACAGGGGGGAGTAAACAGC
  AGACAGAGAAGCCAGAGTCGGAAGATGAGTGGGAACGGACAGAGCGTGAACGCCTTCAGGACCTGGAGGA
  GCGTGATGCCTTTGCTGAGCGGGTTCGACAGCGGGACAAGGATCGGACTCGAAATGTCCTGGAACGGTCA
  GACAAGAAGGCTTATGAAGAGGCTCAGAAGCGCCTCAAGATGGCCGAGGAAGACCGGAAGGCCATGGTCC
  CTGAGCTGCGGAAGAAATCTCGCCGAGAGTACCTGGCTAAGCGGGAGCGAGAGAAGCTTGAGGACCTGGA
  GGCGGAGCTGGCTGATGAGGAGTTCCTTTTTGGGGACGTGGAGCTGAGCCGGCACGAGCGGCAGGAGCTC
  AAATATAAGCGGCGAGTGCGGGATCTCGCCCGGGAGTACCGGGCAGCTGGGGAGCAGGAGAAGCTGGAGG
  CCACCAATCGCTACCACATGCCCAAGGAAACCCGAGGACAGCCAGCCCGAGCTGTGGATCTAGTGGAGGA
  GGAATCAGGAGCCCCTGGGGAGGAGCAGCGGCGCTGGGAGGAGGCGCGGCTTGGGGCAGCGTCCCTGAAG
  TTTGGGGCCCGAGATGCTGCCTCTCAGGAGCCCAAGTATCAACTGGTGCTGGAGGAGGAGGAGACCATTG
  AGTTTGTCCGGGCCACTCAGCTCCAGGGTGATGAGGAGCCGTCAGCTCCACCCACTTCAACTCAGGCCCA
  GCAGAAAGAGTCCATCCAGGCCGTCCGCCGCAGCCTCCCGGTGTTCCCATTTCGAGAGGAGCTCCTGGCT
  GCTATTGCAAATCACCAAGTCCTCATCATTGAAGGCGAGACAGGCTCAGGGAAGACCACCCAGATCCCGC
  AGTATCTCTTTGAGGAGGGTTATACAAACAAGGGTATGAAGATTGCCTGCACCCAACCCCGGAGAGTGGC
  TGCCATGAGTGTGGCCGCCCGAGTGGCCCGGGAGATGGGTGTGAAGCTTGGGAATGAGGTTGGCTACAGC
  ATCCGCTTTGAGGACTGCACATCAGAGCGAACTGTCCTCCGCTACATGACAGATGGGATGCTTCTCCGGG
  AGTTCCTCTCTGAGCCTGACCTGGCGAGTTACAGCGTGGTGATGGTGGATGAGGCACACGAAAGGACCCT
  ACACACAGACATTCTCTTTGGATTGATCAAGGATGTTGCTCGCTTCCGACCTGAGCTCAAGGTCCTGGTG
  GCTTCAGCCACAATGGACACTGCCCGTTTTTCCACCTTCTTTGATGACGCCCCTGTGTTTCGAATCCCCG
  GACGCAGGTTTCCTGTGGACATCTTCTACACCAAGGCTCCAGAGGCTGACTACTTGGAAGCTTGTGTAGT
  ATCTGTGTTGCAGATCCATGTGACCCAGCCCCCTGGGGATATCCTGGTGTTCCTGACAGGACAGGAGGAG
  ATTGAGGCTGCCTGTGAGATGCTCCAGGATCGCTGCCGCCGCCTGGGCTCCAAAATCCGGGAGCTCCTGG
  TGCTGCCCATTTATGCCAATCTGCCCTCTGACATGCAGGCCCGTATCTTCCAGCCCACACCACCTGGGGC
  ACGAAAGGTGGTTGTGGCAACGAACATTGCTGAGACATCACTCACCATTGAGGGCATCATTTATGTGCTG
  GATCCAGGGTTCTGTAAGCAGAAGAGCTACAACCCCCGCACAGGCATGGAATCGCTCACTGTCACACCCT
  GCAGCAAGGCCTCAGCCAATCAGCGAGCTGGCAGGGCAGGTCGGGTGGCTGCAGGGAAGTGCTTCCGCCT
  GTATACCGCCTGGGCCTATCAGCACGAGCTTGAGGAAACCACAGTGCCTGAGATCCAGAGGACCAGCTTG
  GGCAATGTCGTGTTGCTGCTCAAGAGCTTAGGGATCCATGACCTAATGCACTTTGATTTCCTGGACCCTC
  CACCATATGAGACACTGCTGCTGGCTTTGGAGCAGCTGTATGCTCTGGGAGCCCTCAACCACCTTGGGGA
  GCTCACCACGTCTGGTCGAAAGATGGCAGAGCTGCCGGTGGACCCCATGCTGTCCAAAATGATCTTAGCC
  TCTGAGAAGTACAGCTGTTCAGAGGAGATCCTGACAGTGGCTGCCATGCTCTCTGTCAACAACTCCATCT
  TCTACCGACCAAAGGACAAGGTCGTCCATGCTGACAATGCCCGTGTCAACTTCTTTCTCCCTGGCGGTGA
  CCACCTGGTTCTGCTAAATGTTTACACACAGTGGGCTGAGAGTGGTTACTCTTCCCAGTGGTGCTATGAG
  AACTTTGTACAGTTCAGATCGATGCGCCGAGCCCGGGATGTGCGGGAACAGCTGGAAGGGCTCTTGGAAC
  GTGTGGAAGTTGGTCTCAGTTCCTGCCAGGGGGACTATATCCGTGTACGCAAGGCCATCACTGCTGGTTA
  CTTTTACCACACGGCACGGTTGACTCGGAGTGGCTACCGCACAGTGAAACAGCAGCAGACAGTCTTCATT
  CATCCCAACTCCTCCCTCTTTGAGCAACAGCCACGCTGGCTGCTCTACCACGAACTTGTCTTGACCACCA
  AAGAGTTCATGAGACAGGTACTGGAGATTGAGAGCAGTTGGCTTCTGGAGGTGGCTCCCCATTATTATAA
  GGCCAAGGAGCTAGAAGATCCCCATGCTAAGAAAATGCCCAAAAAAATAGGCAAAACACGAGAAGAGCTA
  GGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228627 representing NM_001164239
  Red=Cloning site Green=Tags(s)
  
  MSEDPRGPKVPRKAVVEKPARAAEREARALLEKNRSYRLLEDSEESSEETVSRAGSSLQKKRKKRKHLRK
  KREEEEEEEASEKGKKKTGGSKQQTEKPESEDEWERTERERLQDLEERDAFAERVRQRDKDRTRNVLERS
  DKKAYEEAQKRLKMAEEDRKAMVPELRKKSRREYLAKREREKLEDLEAELADEEFLFGDVELSRHERQEL
  KYKRRVRDLAREYRAAGEQEKLEATNRYHMPKETRGQPARAVDLVEEESGAPGEEQRRWEEARLGAASLK
  FGARDAASQEPKYQLVLEEEETIEFVRATQLQGDEEPSAPPTSTQAQQKESIQAVRRSLPVFPFREELLA
  AIANHQVLIIEGETGSGKTTQIPQYLFEEGYTNKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYS
  IRFEDCTSERTVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLV
  ASATMDTARFSTFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHVTQPPGDILVFLTGQEE
  IEAACEMLQDRCRRLGSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGIIYVL
  DPGFCKQKSYNPRTGMESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSL
  GNVVLLLKSLGIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILA
  SEKYSCSEEILTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYE
  NFVQFRSMRRARDVREQLEGLLERVEVGLSSCQGDYIRVRKAITAGYFYHTARLTRSGYRTVKQQQTVFI
  HPNSSLFEQQPRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKELEDPHAKKMPKKIGKTREEL
  G
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164239
• ORF Size: 2943 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164239.1, NP_001157711.1
• RefSeq ORF: 2946
• Locus ID: 8449
• Protein Pathways: Spliceosome
• MW: 112.3 kDa
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a functional homolog of fission yeast Prp8 protein involved in cell cycle progression. This gene is mapped to the MHC region on chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2018]