Acinus (ACIN1) (NM_001164815) Human Tagged ORF Clone

CAT#: RC228670

ACIN1 (Myc-DDK-tagged)-Human apoptotic chromatin condensation inducer 1 (ACIN1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACIN1
• Synonyms: ACINUS; ACN; fSAP152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228670 representing NM_001164815
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGACGGAAACATCCGAGGACATCCGGAGGAACCCGGGGAGTTCTGAGTGGTAATCGAGGGGTAG
  AGTATGGCAGTGGGCGGGGTCATCTCGGTACGTTCGAAGGGCGTTGGCGGAAATTACCGAAGATGCCCGA
  AGCCGTCGGGACGGACCCGAGTACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTGGACGGGAAG
  CCTCTTCAGGCGCTGCGGGTGACCGACCTGAAGGCCGCACTGGAGCAGCGAGGCCTAGCCAAGAGCGGGC
  AGAAGAGTGCCCTGGTCAAGCGGCTCAAAGGGGCTCTAATGCTAGAAAATTTACAGAAACACTCAACACC
  CCATGCTGCATTCCAGCCAAATTCCCAGATTGGTGAGGAAATGAGCCAGAACAGTTTCATAAAACAGTAT
  CTGGAAAAGCAGCAGGAGCTACTTAGGCAGCGTCTGGAACGTGAAGCTCGAGAAGCTGCAGAACTTGAAG
  GAAAAAGCTCCTCAATTTCTGAAGAGAAAGGTGACTCTGATGATGAGAAACCAAGGAAAGGAGAAAGACG
  ATCATCTAGGGTCAGACAGGCAAGAGCAGCTAAACTGTCTGAGGGCAGCCAACCTGCTGAGGAGGAAGAG
  GATCAAGAAACACCTTCCAGAAACCTAAGGGTCAGAGCAGATCGAAATTTGAAAACAGAGGAGGAAGAAG
  AGGAGGAGGAGGAGGAGGAAGAAGATGATGAAGAAGAGGAAGGTGATGATGAGGGACAAAAATCTAGGGA
  GGCACCAATCCTGAAAGAGTTTAAGGAAGAAGGGGAAGAGATACCTAGAGTAAAACCAGAGGAGATGATG
  GATGAGAGACCCAAAACAAGATCCCAGGAACAGGAGGTGTTAGAGAGAGGAGGGAGATTTACAAGATCCC
  AGGAAGAGGCTAGAAAAAGTCATCTGGCCAGACAGCAGCAGGAGAAGGAAATGAAAACAACATCTCCCCT
  TGAGGAGGAAGAAAGAGAAATAAAATCTTCACAAGGCTTAAAGGAAAAATCGAAGTCTCCTTCCCCTCCT
  CGACTGACTGAAGATCGAAAGAAGGCCTCACTTGTAGCGCTGCCAGAGCAAACTGCCAGCGAGGAGGAGA
  CTCCTCCACCTTTACTAACAAAGGAAGCATCTTCTCCACCACCTCATCCACAGCTCCATAGCGAAGAAGA
  AATAGAGCCCATGGAAGGCCCAGCCCCCCCTGTCCTCATTCAGTTATCTCCTCCTAATACAGATGCTGAC
  ACCAGGGAGCTATTAGTATCTCAGCATACTGTCCAGTTGGTAGGAGGCCTGTCTCCTTTGTCAAGTCCTT
  CAGACACCAAAGCAGAATCTCCAGCAGAGAAAGTGCCAGAGGAGAGTGTCCTGCCTCTGGTTCAGAAAAG
  CACACTGGCTGACTACTCAGCCCAGAAGGATCTTGAACCTGAGTCAGACAGATCTGCTCAGCCCCTCCCT
  CTAAAAATTGAGGAATTAGCACTGGCCAAAGGAATCACTGAAGAATGTCTGAAACAGCCATCTTTGGAAC
  AGAAGGAAGGCAGAAGAGCTTCTCATACCCTTCTCCCAAGCCACAGATTGAAACAGTCAGCTGATTCATC
  CTCTAGCCGGTCCTCCTCATCTTCCTCCTCCAGTTCTAGATCAAGATCTCGCTCTCCTGACAGTTCAGGT
  TCTCGGTCTCATTCACCGCTCAGATCCAAGCAGAGAGATGTAGCCCAGGCACGTACTCATGCCAACCCTC
  GTGGTAGACCCAAGATGGGCTCCAGATCAACATCAGAGTCCAGATCAAGGTCACGTTCACGTTCTCGTTC
  AGCATCAAGCAACAGCAGAAAATCTCTGAGCCCTGGAGTCTCCAGGGACAGCAGCACCAGCTATACTGAA
  ACCAAAGATCCCTCTTCTGGTCAGGAGGTTGCAACTCCACCAGTGCCACAACTGCAGGTCTGTGAGCCAA
  AGGAGAGGACTTCCACCTCCTCATCCTCTGTCCAAGCAAGGCGTCTGAGTCAGCCTGAATCAGCTGAAAA
  GCATGTGACCCAGAGGTTACAGCCTGAGCGGGGGAGCCCAAAGAAGTGTGAAGCTGAAGAGGCAGAGCCA
  CCAGCTGCCACACAGCCCCAAACCTCAGAGACTCAGACCTCTCATCTGCCAGAATCAGAAAGAATTCATC
  ACACTGTTGAGGAGAAGGAGGAAGTGACCATGGACACAAGTGAAAACAGACCTGAAAATGATGTTCCAGA
  ACCTCCCATGCCTATTGCAGACCAAGTCAGCAATGATGACCGCCCGGAGGGCAGTGTTGAAGATGAGGAG
  AAGAAAGAGAGCTCGCTGCCCAAATCATTCAAGAGGAAGATCTCCGTTGTCTCAGCTACCAAGGGGGTGC
  CAGCTGGAAACAGTGACACAGAGGGGGGCCAGCCTGGTCGGAAACGACGCTGGGGAGCCAGCACAGCCAC
  CACACAGAAGAAACCTTCCATCAGTATCACCACTGAATCACTAAAGAGCCTCATCCCCGACATCAAACCC
  CTGGCGGGGCAGGAGGCTGTTGTGGATCTTCATGCTGATGACTCTCGCATCTCTGAGGATGAGACAGAGC
  GTAATGGCGATGATGGGACCCATGACAAGGGGCTGAAAATATGCCGGACAGTCACTCAGGTAGTACCTGC
  AGAGGGCCAGGAGAATGGGCAGAGGGAAGAAGAGGAAGAAGAGAAGGAACCTGAAGCAGAACCTCCTGTA
  CCTCCCCAGGTGTCAGTAGAGGTGGCCTTGCCCCCACCTGCAGAGCATGAAGTAAAGAAAGTGACTTTAG
  GAGATACCTTAACTCGACGTTCCATTAGCCAGCAGAAGTCCGGAGTTTCCATTACCATTGATGACCCAGT
  CCGAACTGCCCAGGTGCCCTCCCCACCCCGGGGCAAGATTAGCAACATTGTCCATATCTCCAATTTGGTC
  CGTCCTTTCACTTTAGGCCAGCTAAAGGAGTTGTTGGGGCGCACAGGAACCTTGGTGGAAGAGGCCTTCT
  GGATTGACAAGATCAAATCTCATTGCTTTGTAACGTACTCAACAGTAGAGGAAGCTGTTGCCACCCGCAC
  AGCTCTGCACGGGGTCAAATGGCCCCAGTCCAATCCCAAATTCCTTTGTGCTGACTATGCCGAGCAAGAT
  GAGCTGGATTATCACCGAGGCCTCTTGGTGGACCGTCCCTCTGAAACTAAGACAGAGGAGCAGGGAATAC
  CACGGCCCCTGCACCCCCCACCCCCACCCCCGGTCCAGCCACCACAGCACCCCCGGGCAGAGCAGCGGGA
  GCAGGAACGGGCAGTGCGGGAACAGTGGGCAGAACGGGAACGGGAAATGGAGCGGCGGGAGCGGACTCGA
  TCAGAGCGTGAATGGGATCGGGACAAAGTTCGAGAAGGGCCCCGTTCCCGATCAAGGTCCCGTGACCGCC
  GCCGCAAGGAACGTGCGAAGTCTAAAGAAAAGAAGAGTGAGAAGAAAGAGAAAGCCCAGGAGGAACCACC
  TGCCAAGCTGCTGGATGACCTTTTCCGAAAGACCAAGGCAGCTCCCTGCATCTATTGGCTCCCACTGACT
  GACAGCCAGATCGTTCAGAAAGAGGCAGAGCGGGCCGAACGGGCCAAGGAGCGGGAGAAGCGGCGAAAGG
  AGCAAGAAGAAGAAGAGCAAAAGGAGCGGGAGAAGGAAGCCGAGCGGGAACGGAACCGACAGCTGGAGCG
  AGAGAAACGTCGGGAGCACAGTCGGGAGAGGGACAGGGAGAGAGAGAGAGAAAGGGAGCGGGACAGGGGG
  GACCGAGATCGGGATAGGGAAAGGGACCGAGAACGAGGCAGGGAAAGGGATCGCAGGGACACCAAGCGCC
  ACAGCAGAAGCCGGAGTCGGAGCACACCTGTGCGGGACCGGGGTGGGCGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228670 representing NM_001164815
  Red=Cloning site Green=Tags(s)
  
  MWRRKHPRTSGGTRGVLSGNRGVEYGSGRGHLGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGK
  PLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQY
  LEKQQELLRQRLEREAREAAELEGKSSSISEEKGDSDDEKPRKGERRSSRVRQARAAKLSEGSQPAEEEE
  DQETPSRNLRVRADRNLKTEEEEEEEEEEEEDDEEEEGDDEGQKSREAPILKEFKEEGEEIPRVKPEEMM
  DERPKTRSQEQEVLERGGRFTRSQEEARKSHLARQQQEKEMKTTSPLEEEEREIKSSQGLKEKSKSPSPP
  RLTEDRKKASLVALPEQTASEEETPPPLLTKEASSPPPHPQLHSEEEIEPMEGPAPPVLIQLSPPNTDAD
  TRELLVSQHTVQLVGGLSPLSSPSDTKAESPAEKVPEESVLPLVQKSTLADYSAQKDLEPESDRSAQPLP
  LKIEELALAKGITEECLKQPSLEQKEGRRASHTLLPSHRLKQSADSSSSRSSSSSSSSSRSRSRSPDSSG
  SRSHSPLRSKQRDVAQARTHANPRGRPKMGSRSTSESRSRSRSRSRSASSNSRKSLSPGVSRDSSTSYTE
  TKDPSSGQEVATPPVPQLQVCEPKERTSTSSSSVQARRLSQPESAEKHVTQRLQPERGSPKKCEAEEAEP
  PAATQPQTSETQTSHLPESERIHHTVEEKEEVTMDTSENRPENDVPEPPMPIADQVSNDDRPEGSVEDEE
  KKESSLPKSFKRKISVVSATKGVPAGNSDTEGGQPGRKRRWGASTATTQKKPSISITTESLKSLIPDIKP
  LAGQEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVTQVVPAEGQENGQREEEEEEKEPEAEPPV
  PPQVSVEVALPPPAEHEVKKVTLGDTLTRRSISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLV
  RPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQD
  ELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWAEREREMERRERTR
  SEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLT
  DSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRERDRERERERERDRG
  DRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164815
• ORF Size: 3903 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164815.1, NP_001158287.1
• RefSeq ORF: 3906
• Locus ID: 22985
• Protein Pathways: Spliceosome
• MW: 147.2 kDa
• Gene Summary: Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. It may thus be involved in mRNA metabolism associated with splicing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]