PEG3 (NM_001146184) Human Tagged ORF Clone

CAT#: RC228688

PEG3 (Myc-DDK-tagged)-Human paternally expressed 3 (PEG3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY431717) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228689 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PEG3
• Synonyms: PW1; ZKSCAN22; ZNF904; ZSCAN24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228688 representing NM_001146184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCTCCAAAGCACTTGTCTGCCACCAAACCTAAGAAGTCCTGGGCCCCAAATCTGTATGAGCTAG
  ACAGTGACTTGACTAAGGAGCCGGATGTCATCATAGGAGAAGGTCCAACTGACTCTGAGTTTTTTCATCA
  GAGGTTTCGGAACCTAATCTATGTGGAATTTGTTGGGCCTCGGAAGACCCTGATCAAACTCCGAAACCTC
  TGCCTCGATTGGTTGCAGCCGGAGACCCGCACCAAGGAGGAGATCATCGAGCTCTTGGTCCTTGAGCAGT
  ACCTGACCATCATCCCTGAAAAGCTCAAGCCTTGGGTGCGAGCAAAAAAGCCGGAGAACTGTGAGAAGCT
  CGTCACTCTGCTGGAGAATTACAAGGAGATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGT
  GACGACGACATGACCCGGAACAGAAGAGAGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGGTGACC
  GGGACTGGGACCGGAGGGGCAGAAGCAGAGACATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCC
  AAGAAGCAGGATGCCTCCGCGGGATCTTTCCCTTCCTGTGGTGGCGAAAACAAGCTTTGAAATGGACAGA
  GAGGACGACAGGGACTCCAGGGCTTATGAGTCCCGATCTCAGGATGCTGAATCATACCAAAATGTGGTGG
  ACCTCGCTGAGGACAGGAAACCTCACAACACAATCCAGGACAACATGGAAAACTACAGGAAGCTGCTCTC
  CCTCGGAGTGCAGCTTGCTGAAGACGATGGCCACTCCCACATGACGCAGGGCCACTCATCAAGATCCAAG
  AGAAGTGCCTACCCAAGCACCAGTCGAGGTCTAAAAACTATGCCTGAAGCCAAAAAATCAACCCACCGGC
  GGGGGATTTGTGAAGATGAATCTTCCCACGGAGTGATAATGGAAAAATTCATCAAGGATGTGTCACGCAG
  TTCCAAATCGGGAAGAGCAAGGGAGTCAAGCGACCGGTCACAGAGATTCCCCAGAATGTCAGATGATAAC
  TGGAAGGACATTTCATTGAACAAGAGGGAGTCAGTGATCCAGCAGCGGGTTTATGAAGGGAATGCATTTA
  GGGGAGGCTTTAGGTTTAATTCAACCCTTGTTTCCAGAAAGAGAGTTCTTGAAAGAAAGAGGCGCTATCA
  TTTTGACACAGATGGGAAGGGCTCGATTCACGATCAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGT
  GGTAGTGAGATGAGAAAAGCCATGAGCGTGAGCAGCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGT
  CACAGCCAATTGATTTTGGGGCAATGCCATATGTATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATCTC
  AGAATTTGTTGAGCACCAGATCATGCATACTAGAGAGAACCTCTATGAGTATGGTGAGTCCTTTATCCAC
  AGTGTGGCTGTCAGTGAAGTTCAGAAAAGTCAGGTTGGAGGGAAACGTTTTGAATGTAAGGACTGTGGAG
  AGACCTTCAATAAGAGTGCCGCCTTGGCTGAACATCGGAAGATTCATGCTAGAGGTTATCTTGTGGAATG
  TAAGAATCAGGAATGTGAGGAAGCCTTCATGCCTAGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGC
  AAAGACAAATTCTACGAGTGCAGGGTGTGTAAGGAAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACC
  AGAAAATCCACTTTGGGGATGACAAAGATAATGAGCGTGAACATGAACGTGAACGTGAACGTGAGCGCGG
  GGAAACCTTTAGGCCCAGCCCAGCCCTTAATGAGTTTCAGAAAATGTATGGTAAAGAGAAAATGTACGAA
  TGTAAGGTGTGTGGGGAGACTTTCCTTCATAGCTCATCCCTGAAAGAACATCAGAAAATCCATACTAGAG
  GGAACCCATTTGAAAACAAGGGTAAAGTGTGTGAGGAAACCTTTATTCCTGGTCAGTCCCTTAAAAGGCG
  TCAGAAAACTTACAATAAGGAGAAGCTCTGTGACTTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCA
  GAGCTCAGTGAGCATCAGAAAATTCATTCTCGAAAGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTG
  TCATTCATAGTGGGCCATTCACTGAATCTCAGAAGAGTCATACTATAACAAGACCTCTTGAAAGTGATGA
  GGACGAAAAGGCGTTCACCATTAGCTCTAACCCCTATGAAAACCAGAAGATTCCCACTAAGGAAAATGTC
  TACGAGGCAAAATCATATGAGAGGTCTGTTATTCATAGCTTAGCCTCTGTGGAAGCTCAGAAAAGTCACA
  GTGTAGCAGGGCCCAGTAAACCAAAAGTAATGGCAGAGTCTACCATTCAGAGCTTCGATGCTATCAACCA
  TCAGAGAGTTCGTGCTGGAGGGAACACCTCTGAAGGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTA
  GTGGCTTCCAAACCTCCAAGAAGTCACAATGGAAATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCT
  CCATTTATATCTCAGACCTTAATGATAAGCGACAGAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGGGG
  CAGTAAGAATCGCAACTATGAAGACTCTGTCATACAGAGTGTATTCCGTGCCAAACCTCAGAAAAGTGTT
  CCTGGAGAGGGATCTGGTGAGTTTAAGAAGGATGGCGAATTCTCTGTTCCCAGCTCAAATGTCCGTGAAT
  ACCAGAAGGCTCGTGCTAAAAAGAAATACATTGAGCATAGGAGCAATGAGACCTCTGTAATTCACTCTCT
  GCCTTTTGGTGAACAAACATTTCGCCCTCGAGGGATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTT
  GCTCATAGCTCTGACCTCACTGAGCACCAGAAGATTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACT
  ATGAATGGTCTGTCATTCGCAGCTTGGCCCCTACTGACCCTCAAACAAGTTACGCCCAAGAGCAGTATGC
  TAAAGAGCAAGCGCGGAACAAATGTAAGGACTTCAGACAATTTTTTGCTACCAGCGAAGACCTCAACACA
  AACCAGAAAATCTATGACCAAGAGAAGTCTCATGGCGAGGAGTCTCAAGGCGAGAATACTGATGGGGAGG
  AGACCCACAGCGAGGAGACCCATGGTCAGGAGACAATTGAAGACCCTGTCATTCAAGGCTCAGACATGGA
  AGACCCTCAGAAGGATGACCCTGATGACAAAATCTATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGAT
  CTCACAGACCTCACAGACCATCAGAAAGTCCACAGCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACAC
  ATTCTGTAATTCACACCCATTCCATCAGCGAGTATCAGAGAGATTACACTGGAGAGCAGCTGTATGAATG
  TCCAAAGTGTGGGGAATCTTTTATTCATAGCTCATTCCTTTTCGAGCATCAGAGAATCCATGAACAAGAC
  CAGTTGTATTCCATGAAGGGGTGTGATGATGGTTTTATTGCCCTCTTGCCCATGAAGCCACGGAGGAATC
  GTGCTGCAGAGAGGAATCCTGCTCTTGCTGGGTCGGCCATTCGATGCCTTTTGTGTGGACAAGGCTTCAT
  TCATAGCTCTGCCCTTAATGAGCATATGAGACTTCATAGGGAAGATGATTTACTGGAGCAGAGCCAGATG
  GCTGAGGAAGCTATCATTCCAGGCTTAGCCCTCACTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCT
  TTGAATGTGCAGTCTGTGGAGAATCTTTCGTCAACCCAGCAGAACTTGCAGATCACGTAACTGTTCATAA
  GAATGAGCCCTATGAGTACGGGTCCTCCTATACTCACACCTCATTTCTTACTGAGCCCCTCAAAGGAGCT
  ATACCATTCTATGAATGCAAGGATTGTGGTAAGTCCTTTATTCATAGCACAGTCCTCACTAAACATAAGG
  AGCTTCATCTGGAAGAAGAAGAAGAAGATGAAGCAGCAGCAGCTGCAGCAGCAGCAGCCCAGGAAGTTGA
  AGCCAATGTCCATGTTCCACAAGTAGTTCTGAGGATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAA
  GTGGAGGCTGCCGAGCCAGAAGTGGAGGCTGCTGAGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGG
  CTGAAGGGCCAGATGGAGAGGCTGCAGAGCCCATTGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAGCA
  GCCAAATGGGGATGCTGATGAGCCAGATGGTGCAGGTATTGAAGACCCAGAAGAAAGAGCTGAAGAGCCA
  GAGGGAAAAGCTGAAGAGCCAGAGGGAGATGCCGACGAGCCTGACGGTGTGGGAATTGAAGACCCAGAAG
  AAGGTGAAGATCAAGAGATTCAGGTAGAAGAACCATACTATGACTGCCATGAATGCACAGAAACCTTCAC
  TTCCAGCACAGCATTCAGTGAACACCTGAAAACTCATGCCAGCATGATCATATTTGAGCCTGCAAATGCC
  TTTGGGGAGTGCTCAGGCTACATCGAACGTGCCAGCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGA
  AGTACTTCAAATGTGACGTCTGTGGGCAGCTCTTCAATGACCGCCTGTCCCTCGCCAGACACCAGAATAC
  CCACACTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228688 representing NM_001146184
  Red=Cloning site Green=Tags(s)
  
  MLPPKHLSATKPKKSWAPNLYELDSDLTKEPDVIIGEGPTDSEFFHQRFRNLIYVEFVGPRKTLIKLRNL
  CLDWLQPETRTKEEIIELLVLEQYLTIIPEKLKPWVRAKKPENCEKLVTLLENYKEMYQPEDDNNSDVTS
  DDDMTRNRRESSPPHSVHSFSGDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDLSLPVVAKTSFEMDR
  EDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLGVQLAEDDGHSHMTQGHSSRSK
  RSAYPSTSRGLKTMPEAKKSTHRRGICEDESSHGVIMEKFIKDVSRSSKSGRARESSDRSQRFPRMSDDN
  WKDISLNKRESVIQQRVYEGNAFRGGFRFNSTLVSRKRVLERKRRYHFDTDGKGSIHDQKGCPRKKPFEC
  GSEMRKAMSVSSLSSLSSPSFTESQPIDFGAMPYVCDECGRSFSVISEFVEHQIMHTRENLYEYGESFIH
  SVAVSEVQKSQVGGKRFECKDCGETFNKSAALAEHRKIHARGYLVECKNQECEEAFMPSPTFSELQKIYG
  KDKFYECRVCKETFLHSSALIEHQKIHFGDDKDNEREHERERERERGETFRPSPALNEFQKMYGKEKMYE
  CKVCGETFLHSSSLKEHQKIHTRGNPFENKGKVCEETFIPGQSLKRRQKTYNKEKLCDFTDGRDAFMQSS
  ELSEHQKIHSRKNLFEGRGYEKSVIHSGPFTESQKSHTITRPLESDEDEKAFTISSNPYENQKIPTKENV
  YEAKSYERSVIHSLASVEAQKSHSVAGPSKPKVMAESTIQSFDAINHQRVRAGGNTSEGREYSRSVIHSL
  VASKPPRSHNGNELVESNEKGESSIYISDLNDKRQKIPARENPCEGGSKNRNYEDSVIQSVFRAKPQKSV
  PGEGSGEFKKDGEFSVPSSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRPRGMLYECQECGECF
  AHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCKDFRQFFATSEDLNT
  NQKIYDQEKSHGEESQGENTDGEETHSEETHGQETIEDPVIQGSDMEDPQKDDPDDKIYECEDCGLGFVD
  LTDLTDHQKVHSRKCLVDSREYTHSVIHTHSISEYQRDYTGEQLYECPKCGESFIHSSFLFEHQRIHEQD
  QLYSMKGCDDGFIALLPMKPRRNRAAERNPALAGSAIRCLLCGQGFIHSSALNEHMRLHREDDLLEQSQM
  AEEAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSSYTHTSFLTEPLKGA
  IPFYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVVLRIQGLNVEAAEPE
  VEAAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPDGAGIEDPEERAEEP
  EGKAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHLKTHASMIIFEPANA
  FGECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146184
• ORF Size: 4770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146184.1, NP_001139656.1
• RefSeq ORF: 4767 bp
• Locus ID: 5178
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• MW: 180.6 kDa
• Gene Summary: 'In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]'