GPR17 (NM_001161417) Human Tagged ORF Clone

CAT#: RC228828

GPR17 (Myc-DDK-tagged)-Human G protein-coupled receptor 17 (GPR17), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR17
• Synonyms: DKFZp686M18273
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228828 representing NM_001161417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATCACCAACTTCTCCCTGGCCACGGCAGAGCAATGTG
  GCCAGGAGACGCCACTGGAGAACATGCTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGT
  TGGCAATACCCTGGCTCTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTG
  ATGCATCTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTCTCTG
  GGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTACCTCAACATGTACGC
  CAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCCATTGTGCACCCGGTCAAGTCCCTC
  AAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGTGCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGG
  CCCCGCTGCTGGTGAGCCCACAGACCGTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGA
  GAAGGCCTCCCACCATGCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACC
  TGCTACCTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAGGCAG
  TGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCACGTCAACCGCTCCGT
  CTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAGCGCATCCTGGCCCTGGCAAACCGC
  ATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTCGACCCCATCATGTATTTCTTCGTGGCTGAGAAGT
  TCCGCCACGCCCTGTGCAACTTGCTCTGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAA
  AACCAACGAGAGCTCGCTGAGTGCCAAGTCAGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228828 representing NM_001161417
  Red=Cloning site Green=Tags(s)
  
  MNGLEVAPPGLITNFSLATAEQCGQETPLENMLFASFYLLDFILALVGNTLALWLFIRDHKSGTPANVFL
  MHLAVADLSCVLVLPTRLVYHFSGNHWPFGEIACRLTGFLFYLNMYASIYFLTCISADRFLAIVHPVKSL
  KLRRPLYAHLACAFLWVVVAVAMAPLLVSPQTVQTNHTVVCLQLYREKASHHALVSLAVAFTFPFITTVT
  CYLLIIRSLRQGLRVEKRLKTKAVRMIAIVLAIFLVCFVPYHVNRSVYVLHYRSHGASCATQRILALANR
  ITSCLTSLNGALDPIMYFFVAEKFRHALCNLLCGKRLKGPPPSFEGKTNESSLSAKSEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161417
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161417.1, NP_001154889.1
• RefSeq Size: 2315 bp
• RefSeq ORF: 1020 bp
• Locus ID: 2840
• Cytogenetics: 2q14.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 37.8 kDa
• Gene Summary: ''