CHFR (NM_001161346) Human Tagged ORF Clone

CAT#: RC228984

CHFR (Myc-DDK-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHFR
• Synonyms: RNF116; RNF196
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228984 representing NM_001161346
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTGCGTCTGGGCG
  CGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGA
  CCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGTGGATGAAAAATCAGGT
  CAGGTGACACTGGAAGATACCAGCACCAGTGGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGA
  CATGCCCTTTACAGACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGC
  ATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGATACCTCAGGTGCAGGT
  GCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTTGAGGAACCAC
  AGCCATCAACATCGACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCAGG
  GCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTG
  GCAAGTGATGAAGTCTCCAGCTTTGCCTCAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGG
  AACCCCAGGATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAA
  CGGGCAGTTGTTGGTCGCACAACCGCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCT
  GGGAAGCCAGACAAGATGGAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGTGA
  GTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTG
  TCCTACCTGCCGCTGTCCCGTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATAC
  CTCATCCAGCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCAGGAATAAAATCA
  CTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCT
  GGAGCTGTCAGACGTTGACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCT
  GAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGG
  CCCTGGGGGATGCACCCTCCACGTCCGTCAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCA
  AGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGTGGAGCGCGAGCAGGAC
  CCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCC
  GGACCGGCTGCTACGGCTGCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGT
  GCTGAACAACAACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAACCAGAGGTTTGACATGGAAA
  AACATGTTGACCGAGAGCCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGG
  GAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTATCGGCAGAA
  CATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAACTGCCGC
  ACTCAGGTGAAAGCTCACCACGCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228984 representing NM_001161346
  Red=Cloning site Green=Tags(s)
  
  MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSG
  QVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAG
  AGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSV
  ASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAA
  GKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAY
  LIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCP
  EYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQD
  PRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWK
  NMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCR
  TQVKAHHAMKFNHICEQTRFKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161346
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161346.1, NP_001154818.1
• RefSeq ORF: 1959
• Locus ID: 55743
• Protein Families: Druggable Genome
• MW: 71.9 kDa
• Gene Summary: This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]