CHFR (NM_001161346) Human Untagged Clone

CAT#: SC327619

CHFR (untagged)-Human checkpoint with forkhead and ring finger domains (CHFR) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHFR
• Synonyms: RNF116; RNF196
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001161346, the custom clone sequence may differ by one or more nucleotides
  ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTG
  CGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATC
  GGGCGGAGACGAGGTTGCGACCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCAC
  TGTAGAATTGTAGTGGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGT
  GGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAGACTGGG
  GATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGCATACCTCTAT
  GAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGATACCTCAGGTGCAGGT
  GCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTT
  GAGGAACCACAGCCATCAACATCGACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCC
  ACGGAGCCTTCTCCTGCAGGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGC
  ATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCTCA
  GCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAGGATCAGGAGGAT
  TTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTG
  TTGGTCGCACAACCGCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCT
  GGGAAGCCAGACAAGATGGAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCAC
  GACTGCGTGAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCTGG
  ATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGGATCTGTAAAAAC
  CACATCCTCAACAACCTCGTGGAAGCATACCTCATCCAGCATCCAGACAAGAGTCGCAGT
  GAAGAAGATGTGCAAAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAGCCC
  AAAGTCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCA
  GACGTTGACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCT
  GAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGA
  GCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGTCAGCCTGACGACAGCAGTCCAG
  GATTACGTGTGCCCTCTGCAAGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATG
  CCCGACCGGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTC
  TGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGC
  CTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAAC
  AACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAACCAGAGGTTTGACATGGAAA
  AACATGTTGACCGAGAGCCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTAC
  AGAGTCACGGGAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTG
  ACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGT
  CCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCACCACGCCATGAAA
  TTCAATCATATCTGTGAACAGACAAGGTTCAAAAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001161346
• ORF Size: 1959 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001161346.1, NP_001154818.1
• RefSeq Size: 3255
• RefSeq ORF: 1959
• Locus ID: 55743
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.