CHFR (NM_001161346) Human Untagged Clone

CAT#: SC327619

CHFR (untagged)-Human checkpoint with forkhead and ring finger domains (CHFR) transcript variant 3


  "NM_001161346" in other vectors (4)

Reconstitution Protocol

USD 1,100.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CHFR
Synonyms RNF116; RNF196
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001161346, the custom clone sequence may differ by one or more nucleotides
ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTG
CGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATC
GGGCGGAGACGAGGTTGCGACCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCAC
TGTAGAATTGTAGTGGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGT
GGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAGACTGGG
GATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGCATACCTCTAT
GAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGATACCTCAGGTGCAGGT
GCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTT
GAGGAACCACAGCCATCAACATCGACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCC
ACGGAGCCTTCTCCTGCAGGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGC
ATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCTCA
GCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAGGATCAGGAGGAT
TTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTG
TTGGTCGCACAACCGCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCT
GGGAAGCCAGACAAGATGGAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCAC
GACTGCGTGAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCTGG
ATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGGATCTGTAAAAAC
CACATCCTCAACAACCTCGTGGAAGCATACCTCATCCAGCATCCAGACAAGAGTCGCAGT
GAAGAAGATGTGCAAAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAGCCC
AAAGTCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCA
GACGTTGACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCT
GAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGA
GCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGTCAGCCTGACGACAGCAGTCCAG
GATTACGTGTGCCCTCTGCAAGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATG
CCCGACCGGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTC
TGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGC
CTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAAC
AACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAACCAGAGGTTTGACATGGAAA
AACATGTTGACCGAGAGCCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTAC
AGAGTCACGGGAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTG
ACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGT
CCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCACCACGCCATGAAA
TTCAATCATATCTGTGAACAGACAAGGTTCAAAAAC
Restriction Sites Please inquire     
ACCN NM_001161346
ORF Size 1959 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Reference Data
RefSeq NM_001161346.1, NP_001154818.1
RefSeq Size 3255
RefSeq ORF 1959
Locus ID 55743
Protein Families Druggable Genome
Gene Summary This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]
Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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