ZBTB20 (NM_001164344) Human Tagged ORF Clone

CAT#: RC228989

ZBTB20 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 20 (ZBTB20), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY432019) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228992 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB20
• Synonyms: DPZF; HOF; ODA-8S; PRIMS; ZNF288
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228989 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGCGCATTCACAGCATCAACCTTCACAACTTCAGCAATTCCGTGCTCGAGACCCTCAACGAGC
  AGCGCAACCGTGGCCACTTCTGTGACGTAACGGTGCGCATCCACGGGAGCATGCTGCGCGCACACCGCTG
  CGTGCTGGCAGCCGGCAGCCCCTTCTTCCAGGACAAACTGCTGCTTGGCTACAGCGACATCGAGATCCCG
  TCGGTGGTGTCAGTGCAGTCAGTGCAAAAGCTCATTGACTTCATGTACAGCGGCGTGCTACGGGTCTCGC
  AGTCGGAAGCTCTGCAGATCCTCACGGCCGCCAGCATCCTGCAGATCAAAACAGTCATCGACGAGTGCAC
  GCGCATCGTGTCACAGAACGTGGGCGATGTGTTCCCGGGGATCCAGGACTCGGGCCAGGACACGCCGCGG
  GGCACTCCCGAGTCAGGCACGTCAGGCCAGAGCAGCGACACGGAGTCGGGCTACCTGCAGAGCCACCCAC
  AGCACAGCGTGGACAGGATCTACTCGGCACTCTACGCGTGCTCCATGCAGAATGGCAGCGGCGAGCGCTC
  TTTTTACAGCGGCGCAGTGGTCAGCCACCACGAGACTGCGCTCGGCCTGCCCCGCGACCACCACATGGAA
  GACCCCAGCTGGATCACACGCATCCATGAGCGCTCGCAGCAGATGGAGCGCTACCTGTCCACCACCCCCG
  AGACCACGCACTGCCGCAAGCAGCCCCGGCCTGTGCGCATCCAGACCCTAGTGGGCAACATCCACATCAA
  GCAGGAGATGGAGGACGATTACGACTACTACGGGCAGCAAAGGGTGCAGATCCTGGAACGCAACGAATCC
  GAGGAGTGCACGGAAGACACAGACCAGGCCGAGGGCACCGAGAGTGAGCCCAAAGGTGAAAGCTTCGACT
  CGGGCGTCAGCTCCTCCATAGGCACCGAGCCTGACTCGGTGGAGCAGCAGTTTGGGCCTGGGGCGGCGCG
  GGACAGCCAGGCTGAACCCACCCAACCCGAGCAGGCTGCAGAAGCCCCCGCTGAGGGTGGTCCGCAGACA
  AACCAGCTAGAAACAGGTGCTTCCTCTCCGGAGAGAAGCAATGAAGTGGAGATGGACAGCACTGTTATCA
  CTGTCAGCAACAGCTCCGACAAGAGCGTCCTACAACAGCCTTCGGTCAACACGTCCATCGGGCAGCCATT
  GCCAAGTACCCAGCTCTACTTACGCCAGACAGAAACCCTCACCAGCAACCTGAGGATGCCTCTGACCTTG
  ACCAGCAACACGCAGGTCATTGGCACAGCTGGCAACACCTACCTGCCAGCCCTCTTCACTACCCAGCCCG
  CGGGCAGTGGCCCCAAGCCTTTCCTCTTCAGCCTGCCACAGCCCCTGGCAGGCCAGCAGACCCAGTTTGT
  GACAGTGTTCCAGCCCGGTCTGTCGACCTTTACTGCACAGCTGCCAGCGCCACAGCCCCTGGCCTCATCC
  GCAGGCCACAGCACAGCCAGTGGGCAAGGCGAAAAAAAGCCTTATGAGTGCACTCTCTGCAACAAGACTT
  TCACCGCCAAACAGAACTACGTCAAGCACATGTTCGTACACACAGGTGAGAAGCCCCACCAATGCAGCAT
  CTGTTGGCGCTCCTTCTCCTTAAAGGATTACCTTATCAAGCACATGGTGACACACACAGGAGTGAGGGCA
  TACCAGTGTAGTATCTGCAACAAGCGCTTCACCCAGAAGAGCTCCCTCAACGTGCACATGCGCCTCCACC
  GGGGAGAGAAGTCCTACGAGTGCTACATCTGCAAAAAGAAGTTCTCTCACAAGACCCTCCTGGAGCGACA
  CGTGGCCCTGCACAGTGCCAGCAATGGGACCCCCCCTGCAGGCACACCCCCAGGTGCCCGCGCTGGCCCC
  CCAGGCGTGGTGGCCTGCACGGAGGGGACCACTTACGTCTGCTCCGTCTGCCCAGCAAAGTTTGACCAAA
  TCGAGCAGTTCAACGACCACATGAGGATGCATGTGTCTGACGGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228989 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTERIHSINLHNFSNSVLETLNEQRNRGHFCDVTVRIHGSMLRAHRCVLAAGSPFFQDKLLLGYSDIEIP
  SVVSVQSVQKLIDFMYSGVLRVSQSEALQILTAASILQIKTVIDECTRIVSQNVGDVFPGIQDSGQDTPR
  GTPESGTSGQSSDTESGYLQSHPQHSVDRIYSALYACSMQNGSGERSFYSGAVVSHHETALGLPRDHHME
  DPSWITRIHERSQQMERYLSTTPETTHCRKQPRPVRIQTLVGNIHIKQEMEDDYDYYGQQRVQILERNES
  EECTEDTDQAEGTESEPKGESFDSGVSSSIGTEPDSVEQQFGPGAARDSQAEPTQPEQAAEAPAEGGPQT
  NQLETGASSPERSNEVEMDSTVITVSNSSDKSVLQQPSVNTSIGQPLPSTQLYLRQTETLTSNLRMPLTL
  TSNTQVIGTAGNTYLPALFTTQPAGSGPKPFLFSLPQPLAGQQTQFVTVFQPGLSTFTAQLPAPQPLASS
  AGHSTASGQGEKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTGVRA
  YQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSASNGTPPAGTPPGARAGP
  PGVVACTEGTTYVCSVCPAKFDQIEQFNDHMRMHVSDG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164344
• ORF Size: 2004 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164344.1, NM_001164344.2, NP_001157816.1
• RefSeq Size: 27337 bp
• RefSeq ORF: 2007 bp
• Locus ID: 26137
• Protein Families: Transcription Factors
• MW: 73.5 kDa
• Gene Summary: This gene, which was initially designated as dendritic cell-derived BTB/POZ zinc finger (DPZF), belongs to a family of transcription factors with an N-terminal BTB/POZ domain and a C-terminal DNA-bindng zinc finger domain. The BTB/POZ domain is a hydrophobic region of approximately 120 aa which mediates association with other BTB/POZ domain-containing proteins. This gene acts as a transcriptional repressor and plays a role in many processes including neurogenesis, glucose homeostasis, and postnatal growth. Mutations in this gene have been associated with Primrose syndrome as well as the 3q13.31 microdeletion syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]