RASGRP3 (NM_015376) Human Tagged ORF Clone

CAT#: RC228997

RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RASGRP3
• Synonyms: GRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228997 representing NM_015376
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATTGAGATGTTTG
  ATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTGATGCACCGATGGTATTTATC
  TTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGAAATGCCACTGGAGAAAGCTGCAATGAATTT
  CGATTAAAGATCTGCTACTTCATGAGGTACTGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGATCTTG
  GTTTGATTCGTATGACTGAGGAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTCAGCCT
  CATCGACATATCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTATCCAAG
  AAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTCACTTTTCTGG
  AGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTCATCCATGGCTGCCTGGAGAA
  TAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGAATCTCTAAGTGGGTCCAGTTGATGGTTCTT
  AGCAAACCAACCCCCCAGCAAAGGGCAGAAGTCATCACAAAGTTTATCAATGTTGCAAAGAAGCTCCTTC
  AGCTCAAAAATTTTAACACCCTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATTTCACGCCTCAA
  AGAGACCCATTCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAGTTGGTCTCCTCC
  AACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATCCCCATCCTTGGAG
  TACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACAGAGGAGAACAAAGTGAACAT
  TGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTAGTCTCCCTGCAGAATGCCTCTCACCACTTA
  GAACCCAACATGGATTTGATCAACCTGCTCACGCTTTCCCTGGACCTCTATCACACTGAAGATGATATTT
  ACAAACTGTCACTGGTGCTGGAGCCTAGAAATTCTAAATCGCCTACCTCCCCTACGACGCCCAACAAGCC
  TGTGGTACCCCTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCCACGGTCATCAACAAGCACATA
  AGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGATGGGTACATTTCCCAAGAGGACT
  TTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTTCTGGACAAAGATCAGGATGGCCT
  AATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCTAAATCCCAACTACACTGTAAAATGGGACCA
  GGATTTATCCATAATTTTCAGGAGATGACCTATCTCAAGCCAACCTTCTGCGAACACTGTGCGGGATTTC
  TCTGGGGCATAATCAAGCAAGGATACAAATGCAAAGACTGTGGAGCCAATTGTCACAAACAGTGCAAAGA
  CCTCCTGGTTCTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCCTTGAGCAGTGGTCATGGGTCACTGCCT
  GGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAGTTCCCTGGAGTCACTGCTGGACACAGGG
  ATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCTCGCAAGATCTCTGTGAGGCTACAGAGGGC
  CACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCAGAGGCTGGCTGGGGGGACTCGGGGTCCCAC
  ACCTTCCCTAAAATGAAATCCAAGTTCCATGACAAAGCAGCAAAGGACAAAGGCTTTGCCAAATGGGAAA
  ATGAGAAGCCCAGGGTGCATGCTGGTGTGGATGTTGTAGACCGGGGCACGGAGTTTGAACTTGACCAGGA
  TGAAGGAGAAGAGACCAGACAGGATGGTGAGGATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228997 representing NM_015376
  Red=Cloning site Green=Tags(s)
  
  MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRWYLSSTELAEKLLCMYRNATGESCNEF
  RLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGYEKHVSLIDISSIPSYDWMRRVTQRKKVSK
  KGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVL
  SKPTPQQRAEVITKFINVAKKLLQLKNFNTLMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTELVSS
  NGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLSVTLSELVSLQNASHHL
  EPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSPTSPTTPNKPVVPLEWALGVMPKPDPTVINKHI
  RKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGP
  GFIHNFQEMTYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSLP
  GSPSLPPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAGWGDSGSH
  TFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEETRQDGEDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015376
• ORF Size: 2067 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015376.1, NM_015376.2, NP_056191.1
• RefSeq Size: 4353
• RefSeq ORF: 2070
• Locus ID: 25780
• Domains: RasGEFN, RasGEF, EFh, DAG_PE-bind
• Protein Pathways: B cell receptor signaling pathway, MAPK signaling pathway
• MW: 78.2 kDa
• Gene Summary: The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017]