KCNQ5 (NM_001160130) Human Tagged ORF Clone

CAT#: RC229040

KCNQ5 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNQ5
• Synonyms: Kv7.5; MRD46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229040 representing NM_001160130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCCACCACGCGGGAGGAGAGGAGGGCGGCGCCGCCGGGCTCTGGGTGAAGAGCGGCGCAGCGG
  CGGCGGCGGCGGGCGGGGGGCGCTTGGGCAGCGGCATGAAGGATGTGGAGTCCGGCCGGGGCAGGGTGCT
  GCTGAACTCGGCAGCCGCCAGGGGCGACGGCCTGCTACTGCTGGGCACCCGCGCGGCCACGCTCGGTGGC
  GGCGGCGGTGGCCTGAGGGAGAGCCGCCGGGGCAAGCAGGGGGCCCGGATGAGCCTGCTGGGGAAGCCGC
  TCTCTTACACGAGTAGCCAGAGCTGCCGGCGCAACGTCAAGTACCGGCGGGTGCAGAACTACCTGTACAA
  CGTGCTGGAGAGACCCCGCGGCTGGGCGTTCATCTACCACGCTTTCGTTTTTCTCCTTGTCTTTGGTTGC
  TTGATTTTGTCAGTGTTTTCTACCATCCCTGAGCACACAAAATTGGCCTCAAGTTGCCTCTTGATCCTGG
  AGTTCGTGATGATTGTCGTCTTTGGTTTGGAGTTCATCATTCGAATCTGGTCTGCGGGTTGCTGTTGTCG
  ATATAGAGGATGGCAAGGAAGACTGAGGTTTGCTCGAAAGCCCTTCTGTGTTATAGATACCATTGTTCTT
  ATCGCTTCAATAGCAGTTGTTTCTGCAAAAACTCAGGGTAATATTTTTGCCACGTCTGCACTCAGAAGTC
  TCCGTTTCCTACAGATCCTCCGCATGGTGCGCATGGACCGAAGGGGAGGCACTTGGAAATTACTGGGTTC
  AGTGGTTTATGCTCACAGCAAGGAATTAATCACAGCTTGGTACATAGGATTTTTGGTTCTTATTTTTTCG
  TCTTTCCTTGTCTATCTGGTGGAAAAGGATGCCAATAAAGAGTTTTCTACATATGCAGATGCTCTCTGGT
  GGGGCACAATTACATTGACAACTATTGGCTATGGAGACAAAACTCCCCTAACTTGGCTGGGAAGATTGCT
  TTCTGCAGGCTTTGCACTCCTTGGCATTTCTTTCTTTGCACTTCCTGCCGGCATTCTTGGCTCAGGTTTT
  GCATTAAAAGTACAAGAACAACACCGCCAGAAACACTTTGAGAAAAGAAGGAACCCAGCTGCCAACCTCA
  TTCAGTGTGTTTGGCGTAGTTACGCAGCTGATGAGAAATCTGTTTCCATTGCAACCTGGAAGCCACACTT
  GAAGGCCTTGCACACCTGCAGCCCTACCAATCAGAAGCTAAGTTTTAAGGAGCGAGTGCGCATGGCTAGC
  CCCAGGGGCCAGAGTATTAAGAGCCGACAAGCCTCAGTAGGTGACAGGAGGTCCCCAAGCACCGACATCA
  CAGCCGAGGGCAGTCCCACCAAAGTGCAGAAGAGCTGGAGCTTCAACGACCGAACCCGCTTCCGGCCCTC
  GCTGCGCCTCAAAAGTTCTCAGCCAAAACCAGTGATAGATGCTGACACAGCCCTTGGCACTGATGATGTA
  TATGATGAAAAAGGATGCCAGTGTGATGTATCAGTGGAAGACCTCACCCCACCACTTAAAACTGTCATTC
  GAGCTATCAGAATTATGAAATTTCATGTTGCAAAACGGAAGTTTAAGGAAACATTACGTCCATATGATGT
  AAAAGATGTCATTGAACAATATTCTGCTGGTCATCTGGACATGTTGTGTAGAATTAAAAGCCTTCAAACA
  CGTGTTGATCAAATTCTTGGAAAAGGGCAAATCACATCAGATAAGAAGAGCCGAGAGAAAATAACAGCAG
  AACATGAGACCACAGACGATCTCAGTATGCTCGGTCGGGTGGTCAAGGTTGAAAAACAGGTACAGTCCAT
  AGAATCCAAGCTGGACTGCCTACTAGACATCTATCAACAGGTCCTTCGGAAAGGCTCTGCCTCAGCCCTC
  GCTTTGGCTTCATTCCAGATCCCACCTTTTGAATGTGAACAGACATCTGACTATCAAAGCCCTGTGGATA
  GCAAAGATCTTTCGGGTTCCGCACAAAACAGTGGCTGCTTATCCAGATCAACTAGTGCCAACATCTCGAG
  AGGCCTGCAGTTCATTCTGACGCCAAATGAGTTCAGTGCCCAGACTTTCTACGCGCTTAGCCCTACTATG
  CACAGTCAAGCAACACAGGTGCCAATTAGTCAAAGCGATGGCTCAGCAGTGGCAGCCACCAACACCATTG
  CAAACCAAATAAATACGGCACCCAAGCCAGCAGCCCCAACAACTTTACAGATCCCACCTCCTCTCCCAGC
  CATCAAGCATCTGCCCAGGCCAGAAACTCTGCACCCTAACCCTGCAGGCTTACAGGAAAGCATTTCTGAC
  GTCACCACCTGCCTTGTTGCCTCCAAGGAAAATGTTCAGGTTGCACAGTCAAATCTCACCAAGGACCGTT
  CTATGAGGAAAAGCTTTGACATGGGAGGAGAAACTCTGTTGTCTGTCTGTCCCATGGTGCCGAAGGACTT
  GGGCAAATCTTTGTCTGTGCAAAACCTGATCAGGTCGACCGAGGAACTGAATATACAACTTTCAGGGAGT
  GAGTCAAGTGGCTCCAGAGGCAGCCAAGATTTTTACCCCAAATGGAGGGAATCCAAATTGTTTATAACTG
  ATGAAGAGGTGGGTCCCGAAGAGACAGAGACAGACACTTTTGATGCCGCACCGCAGCCTGCCAGGGAAGC
  TGCCTTTGCATCAGACTCTCTAAGGACTGGAAGGTCACGATCATCTCAGAGCATTTGTAAGGCAGGAGAA
  AGTACAGATGCCCTCAGCTTGCCTCATGTCAAACTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229040 representing NM_001160130
  Red=Cloning site Green=Tags(s)
  
  MPRHHAGGEEGGAAGLWVKSGAAAAAAGGGRLGSGMKDVESGRGRVLLNSAAARGDGLLLLGTRAATLGG
  GGGGLRESRRGKQGARMSLLGKPLSYTSSQSCRRNVKYRRVQNYLYNVLERPRGWAFIYHAFVFLLVFGC
  LILSVFSTIPEHTKLASSCLLILEFVMIVVFGLEFIIRIWSAGCCCRYRGWQGRLRFARKPFCVIDTIVL
  IASIAVVSAKTQGNIFATSALRSLRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELITAWYIGFLVLIFS
  SFLVYLVEKDANKEFSTYADALWWGTITLTTIGYGDKTPLTWLGRLLSAGFALLGISFFALPAGILGSGF
  ALKVQEQHRQKHFEKRRNPAANLIQCVWRSYAADEKSVSIATWKPHLKALHTCSPTNQKLSFKERVRMAS
  PRGQSIKSRQASVGDRRSPSTDITAEGSPTKVQKSWSFNDRTRFRPSLRLKSSQPKPVIDADTALGTDDV
  YDEKGCQCDVSVEDLTPPLKTVIRAIRIMKFHVAKRKFKETLRPYDVKDVIEQYSAGHLDMLCRIKSLQT
  RVDQILGKGQITSDKKSREKITAEHETTDDLSMLGRVVKVEKQVQSIESKLDCLLDIYQQVLRKGSASAL
  ALASFQIPPFECEQTSDYQSPVDSKDLSGSAQNSGCLSRSTSANISRGLQFILTPNEFSAQTFYALSPTM
  HSQATQVPISQSDGSAVAATNTIANQINTAPKPAAPTTLQIPPPLPAIKHLPRPETLHPNPAGLQESISD
  VTTCLVASKENVQVAQSNLTKDRSMRKSFDMGGETLLSVCPMVPKDLGKSLSVQNLIRSTEELNIQLSGS
  ESSGSRGSQDFYPKWRESKLFITDEEVGPEETETDTFDAAPQPAREAAFASDSLRTGRSRSSQSICKAGE
  STDALSLPHVKLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160130
• ORF Size: 2769 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001160130.1, NP_001153602.1
• RefSeq ORF: 2772
• Locus ID: 56479
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 101.1 kDa
• Gene Summary: This gene is a member of the KCNQ potassium channel gene family that is differentially expressed in subregions of the brain and in skeletal muscle. The protein encoded by this gene yields currents that activate slowly with depolarization and can form heteromeric channels with the protein encoded by the KCNQ3 gene. Currents expressed from this protein have voltage dependences and inhibitor sensitivities in common with M-currents. They are also inhibited by M1 muscarinic receptor activation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]