TFII I (GTF2I) (NM_001163636) Human Tagged ORF Clone

CAT#: RC229048

GTF2I (Myc-DDK-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2I
• Synonyms: BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229048 representing NM_001163636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAAGTTGCAATGTCCACCCTCCCCGTTGAAGATGAGGAGTCCTCGGAGAGCAGGATGGTGGTGA
  CATTCCTCATGTCAGCTCTCGAGTCCATGTGTAAAGAACTGGCCAAGTCCAAAGCCGAAGTGGCCTGCAT
  TGCAGTGTATGAAACAGACGTGTTTGTCGTCGGAACTGAAAGAGGACGTGCTTTTGTCAATACCAGAAAG
  GATTTTCAAAAAGATTTTGTAAAATATTGTGTTGAAGAAGAAGAAAAAGCTGCAGAGATGCATAAAATGA
  AATCTACAACCCAGGCAAATCGGATGAGTGTAGATGCTGTAGAAATTGAAACACTCAGAAAAACAGTTGA
  GGACTATTTCTGCTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTACCTGTACCATATGAGAAG
  ATGCTGCGAGACCAGTCGGCTGTGGTAGTGCAGGGGCTTCCGGAAGGTGTTGCCTTTAAACACCCCGAGA
  ACTATGATCTTGCAACCCTGAAATGGATTTTGGAGAACAAAGCAGGGATTTCATTCATCATTAAGAGACC
  TTTTTTAGAGCCAAAGAAGCATGTAGGTGGTCGTGTGATGGTAACAGATGCTGACAGGTCAATACTATCT
  CCAGGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCCCTGGAAA
  TGGCAGCTGTGACAGTAAAGGAAGAATCAGAAGATCCTGATTATTATCAATATAACATTCAAGGCCCTTC
  TGAAACTGATGATGTTGATGAAAAACAGCCCCTATCGAAGCCTTTGCAAGGAAGCCACCATTCTTCAGAG
  GGCAATGAAGGCACAGAAATGGAAGTACCAGCAGAAGATGATGATTATTCTCCACCGTCTAAGAGACCAA
  AGGCCAATGAGCTACCGCAGCCACCAGTCCCGGAACCCGCCAATGCTGGGAAGCGGAAAGTGAGGGAGTT
  CAACTTCGAGAAATGGAATGCTCGCATCACTGATCTACGTAAACAAGTTGAAGAATTGTTTGAAAGGAAA
  TATGCTCAAGCCATAAAAGCCAAAGGTCCGGTGACGATCCCGTACCCTCTTTTCCAGTCTCATGTTGAAG
  ATCTTTATGTAGAAGGACTTCCTGAAGGAATTCCTTTTAGAAGGCCATCTACTTACGGAATTCCTCGCCT
  GGAGAGGATATTACTTGCAAAGGAAAGGATTCGTTTTGTGATTAAGAAACATGAGCTTCTGAATTCAACA
  CGTGAAGATTTACAGCTTGATAAGCCAGCTTCAGGAGTAAAGGAAGAATGGTATGCCAGAATCACTAAAT
  TAAGAAAGATGGTGGATCAGCTTTTCTGCAAAAAATTTGCGGAAGCCTTGGGGAGCACTGAAGCCAAGGC
  TGTACCGTACCAAAAATTTGAGGCACACCCGAATGATCTGTACGTGGAAGGACTGCCAGAAAACATTCCT
  TTCCGAAGTCCCTCATGGTATGGAATCCCAAGGCTGGAAAAAATCATTCAAGTGGGCAATCGAATTAAAT
  TTGTTATTAAAAGACCAGAACTTCTGACTCACAGTACCACTGAAGTTACTCAGCCAAGAACGAATACACC
  AGTCAAAGAAGATTGGAATGTCAGAATTACCAAGCTACGGAAGCAAGTGGAAGAGATTTTTAATTTGAAA
  TTTGCTCAAGCTCTTGGACTCACCGAGGCAGTAAAAGTACCATATCCTGTGTTTGAATCAAACCCGGAGT
  TCTTGTATGTGGAAGGCTTGCCAGAGGGGATTCCCTTCCGAAGCCCTACCTGGTTTGGAATTCCACGACT
  TGAAAGGATCGTCCGCGGGAGTAATAAAATCAAGTTCGTTGTTAAAAAACCTGAACTAGTTATTTCCTAC
  TTGCCTCCTGGGATGGCTAGTAAAATAAACACTAAAGCTTTGCAGTCCCCCAAAAGACCACGAAGTCCTG
  GGAGTAATTCAAAGGTTCCTGAAATTGAGGTCACCGTGGAAGGCCCTAATAACAACAATCCTCAAACCTC
  AGCTGTTCGAACCCCGACCCAGACTAACGGTTCTAACGTTCCCTTCAAGCCACGAGGGAGAGAGTTTTCC
  TTTGAGGCCTGGAATGCCAAAATCACGGACCTAAAACAGAAAGTTGAAAATCTCTTCAATGAGAAATGTG
  GGGAAGCTCTTGGCCTTAAACAAGCTGTGAAGGTGCCGTTCGCGTTATTTGAGTCTTTCCCGGAAGACTT
  TTATGTGGAAGGCTTACCTGAGGGTGTGCCATTCCGAAGACCATCGACTTTTGGCATTCCGAGGCTGGAG
  AAGATACTCAGAAACAAAGCCAAAATTAAGTTCATCATTAAAAAGCCCGAAATGTTTGAGACGGCGATTA
  AGGAGAGCACCTCCTCTAAGAGCCCTCCCAGAAAAATAAATTCATCACCCAATGTTAATACTACTGCATC
  AGGTGTTGAAGACCTTAACATCATTCAGGTGACAATTCCAGATGATGATAATGAAAGACTCTCGAAAGTT
  GAAAAAGCTAGACAGCTAAGAGAACAAGTGAATGACCTCTTTAGTCGGAAATTTGGTGAAGCTATTGGTA
  TGGGTTTTCCTGTGAAAGTTCCCTACAGGAAAATCACAATTAACCGTGGCTGTGTGGTGGTTGATGGCAT
  GCCCCCGGGGGTGTCCTTCAAAGCCCCCAGCTACCTGGAAATCAGCTCCATGAGAAGGATCTTAGACTCT
  GCCGAGTTTATCAAATTCACGGTCATTAGACCATTTCCAGGACTTGTGGTTAATAACCAGCTGGTTGATC
  AGAGTGAGTCAGAAGGCCCCGTGATACAAGAATCAGCTGAACCAAGCCAGTTGGAAGTTCCAGCCACAGA
  AGAAATAAAAGAGACTGATGGAAGCTCTCAGATCAAGCAAGAACCAGACCCCACGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229048 representing NM_001163636
  Red=Cloning site Green=Tags(s)
  
  MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK
  DFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK
  MLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILS
  PGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGPSETDDVDEKQPLSKPLQGSHHSSE
  GNEGTEMEVPAEDDDYSPPSKRPKANELPQPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERK
  YAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNST
  REDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIP
  FRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLK
  FAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVISY
  LPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFS
  FEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLE
  KILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKV
  EKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINRGCVVVDGMPPGVSFKAPSYLEISSMRRILDS
  AEFIKFTVIRPFPGLVVNNQLVDQSESEGPVIQESAEPSQLEVPATEEIKETDGSSQIKQEPDPTW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163636
• ORF Size: 2928 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163636.1, NM_001163636.2, NP_001157108.1
• RefSeq ORF: 2931 bp
• Locus ID: 2969
• Cytogenetics: 7q11.23
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 109.9 kDa
• Gene Summary: 'This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]'