Acinus (ACIN1) (NM_001164814) Human Tagged ORF Clone

CAT#: RC229074

ACIN1 (Myc-DDK-tagged)-Human apoptotic chromatin condensation inducer 1 (ACIN1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACIN1
• Synonyms: ACINUS; ACN; fSAP152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229074 representing NM_001164814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGACGGAAACATCCGAGGACATCCGGAGGAACCCGGGGAGTTCTGAGTGGTAATCGAGGGGTAG
  AGTATGGCAGTGGGCGGGGTCATCTCGGTACGTTCGAAGGGCGTTGGCGGAAATTACCGAAGATGCCCGA
  AGCCGTCGGGACGGACCCGAGTACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTGGACGGGAAG
  CCTCTTCAGGCGCTGCGGGTGACCGACCTGAAGGCCGCACTGGAGCAGCGAGGCCTAGCCAAGAGCGGGC
  AGAAGAGTGCCCTGGTCAAGCGGCTCAAAGGGGCTCTAATGCTAGAAAATTTACAGAAACACTCAACACC
  CCATGCTGCATTCCAGCCAAATTCCCAGATTGGTGAGGAAATGAGCCAGAACAGTTTCATAAAACAGTAT
  CTGGAAAAGCAGCAGGAGCTACTTAGGCAGCGTCTGGAACGTGAAGCTCGAGAAGCTGCAGAACTTGAAG
  AAGCTTCAGCTGAGTCGGAGGACGAGATGATCCATCCTGAGGGAGTGGCTTCCCTGCTGCCTCCTGACTT
  TCAGAGCAGCCTGGAGAGACCAGAGCTGGAGCTCAGCAGACATTCGCCCAGAAAAAGCTCCTCAATTTCT
  GAAGAGAAAGGTGACTCTGATGATGAGAAACCAAGGAAAGGAGAAAGACGATCATCTAGGGTCAGACAGG
  CAAGAGCAGCTAAACTGTCTGAGGGCAGCCAACCTGCTGAGGAGGAAGAGGATCAAGAAACACCTTCCAG
  AAACCTAAGGGTCAGAGCAGATCGAAATTTGAAAACAGAGGAGGAAGAAGAGGAGGAGGAGGAGGAGGAA
  GAAGATGATGAAGAAGAGGAAGGTGATGATGAGGGACAAAAATCTAGGGAGGCACCAATCCTGAAAGAGT
  TTAAGGAAGAAGGGGAAGAGATACCTAGAGTAAAACCAGAGGAGATGATGGATGAGAGACCCAAAACAAG
  ATCCCAGGAACAGGAGGTGTTAGAGAGAGGAGGGAGATTTACAAGATCCCAGGAAGAGGCTAGAAAAAGT
  CATCTGGCCAGACAGCAGCAGGAGAAGGAAATGAAAACAACATCTCCCCTTGAGGAGGAAGAAAGAGAAA
  TAAAATCTTCACAAGGCTTAAAGGAAAAATCGAAGTCTCCTTCCCCTCCTCGACTGACTGAAGATCGAAA
  GAAGGCCTCACTTGTAGCGCTGCCAGAGCAAACTGCCAGCGAGGAGGAGACTCCTCCACCTTTACTAACA
  AAGGAAGCATCTTCTCCACCACCTCATCCACAGCTCCATAGCGAAGAAGAAATAGAGCCCATGGAAGGCC
  CAGCCCCCCCTGTCCTCATTCAGTTATCTCCTCCTAATACAGATGCTGACACCAGGGAGCTATTAGTATC
  TCAGCATACTGTCCAGTTGGTAGGAGGCCTGTCTCCTTTGTCAAGTCCTTCAGACACCAAAGCAGAATCT
  CCAGCAGAGAAAGTGCCAGAGGAGAGTGTCCTGCCTCTGGTTCAGAAAAGCACACTGGCTGACTACTCAG
  CCCAGAAGGATCTTGAACCTGAGTCAGACAGATCTGCTCAGCCCCTCCCTCTAAAAATTGAGGAATTAGC
  ACTGGCCAAAGGAATCACTGAAGAATGTCTGAAACAGCCATCTTTGGAACAGAAGGAAGGCAGAAGAGCT
  TCTCATACCCTTCTCCCAAGCCACAGATTGAAACAGTCAGCTGATTCATCCTCTAGCCGGTCCTCCTCAT
  CTTCCTCCTCCAGTTCTAGATCAAGATCTCGCTCTCCTGACAGTTCAGGTTCTCGGTCTCATTCACCGCT
  CAGATCCAAGCAGAGAGATGTAGCCCAGGCACGTACTCATGCCAACCCTCGTGGTAGACCCAAGATGGGC
  TCCAGATCAACATCAGAGTCCAGATCAAGGTCACGTTCACGTTCTCGTTCAGCATCAAGCAACAGCAGAA
  AATCTCTGAGCCCTGGAGTCTCCAGGGACAGCAGCACCAGCTATACTGAAACCAAAGATCCCTCTTCTGG
  TCAGGAGGTTGCAACTCCACCAGTGCCACAACTGCAGGTCTGTGAGCCAAAGGAGAGGACTTCCACCTCC
  TCATCCTCTGTCCAAGCAAGGCGTCTGAGTCAGCCTGAATCAGCTGAAAAGCATGTGACCCAGAGGTTAC
  AGCCTGAGCGGGGGAGCCCAAAGAAGTGTGAAGCTGAAGAGGCAGAGCCACCAGCTGCCACACAGCCCCA
  AACCTCAGAGACTCAGACCTCTCATCTGCCAGAATCAGAAAGAATTCATCACACTGTTGAGGAGAAGGAG
  GAAGTGACCATGGACACAAGTGAAAACAGACCTGAAAATGATGTTCCAGAACCTCCCATGCCTATTGCAG
  ACCAAGTCAGCAATGATGACCGCCCGGAGGGCAGTGTTGAAGATGAGGAGAAGAAAGAGAGCTCGCTGCC
  CAAATCATTCAAGAGGAAGATCTCCGTTGTCTCTACCAAGGGGGTGCCAGCTGGAAACAGTGACACAGAG
  GGGGGCCAGCCTGGTCGGAAACGACGCTGGGGAGCCAGCACAGCCACCACACAGAAGAAACCTTCCATCA
  GTATCACCACTGAATCACTAAAGGAGGCTGTTGTGGATCTTCATGCTGATGACTCTCGCATCTCTGAGGA
  TGAGACAGAGCGTAATGGCGATGATGGGACCCATGACAAGGGGCTGAAAATATGCCGGACAGTCACTCAG
  GTAGTACCTGCAGAGGGCCAGGAGAATGGGCAGAGGGAAGAAGAGGAAGAAGAGAAGGAACCTGAAGCAG
  AACCTCCTGTACCTCCCCAGGTGTCAGTAGAGGTGGCCTTGCCCCCACCTGCAGAGCATGAAGTAAAGAA
  AGTGACTTTAGGAGATACCTTAACTCGACGTTCCATTAGCCAGCAGAAGTCCGGAGTTTCCATTACCATT
  GATGACCCAGTCCGAACTGCCCAGGTGCCCTCCCCACCCCGGGGCAAGATTAGCAACATTGTCCATATCT
  CCAATTTGGTCCGTCCTTTCACTTTAGGCCAGCTAAAGGAGTTGTTGGGGCGCACAGGAACCTTGGTGGA
  AGAGGCCTTCTGGATTGACAAGATCAAATCTCATTGCTTTGTAACGTACTCAACAGTAGAGGAAGCTGTT
  GCCACCCGCACAGCTCTGCACGGGGTCAAATGGCCCCAGTCCAATCCCAAATTCCTTTGTGCTGACTATG
  CCGAGCAAGATGAGCTGGATTATCACCGAGGCCTCTTGGTGGACCGTCCCTCTGAAACTAAGACAGAGGA
  GCAGGGAATACCACGGCCCCTGCACCCCCCACCCCCACCCCCGGTCCAGCCACCACAGCACCCCCGGGCA
  GAGCAGCGGGAGCAGGAACGGGCAGTGCGGGAACAGTGGGCAGAACGGGAACGGGAAATGGAGCGGCGGG
  AGCGGACTCGATCAGAGCGTGAATGGGATCGGGACAAAGTTCGAGAAGGGCCCCGTTCCCGATCAAGGTC
  CCGTGACCGCCGCCGCAAGGAACGTGCGAAGTCTAAAGAAAAGAAGAGTGAGAAGAAAGAGAAAGCCCAG
  GAGGAACCACCTGCCAAGCTGCTGGATGACCTTTTCCGAAAGACCAAGGCAGCTCCCTGCATCTATTGGC
  TCCCACTGACTGACAGCCAGATCGTTCAGAAAGAGGCAGAGCGGGCCGAACGGGCCAAGGAGCGGGAGAA
  GCGGCGAAAGGAGCAAGAAGAAGAAGAGCAAAAGGAGCGGGAGAAGGAAGCCGAGCGGGAACGGAACCGA
  CAGCTGGAGCGAGAGAAACGTCGGGAGCACAGTCGGGAGAGGGACAGGGAGAGAGAGAGAGAAAGGGAGC
  GGGACAGGGGGGACCGAGATCGGGATAGGGAAAGGGACCGAGAACGAGGCAGGGAAAGGGATCGCAGGGA
  CACCAAGCGCCACAGCAGAAGCCGGAGTCGGAGCACACCTGTGCGGGACCGGGGTGGGCGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229074 representing NM_001164814
  Red=Cloning site Green=Tags(s)
  
  MWRRKHPRTSGGTRGVLSGNRGVEYGSGRGHLGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGK
  PLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQY
  LEKQQELLRQRLEREAREAAELEEASAESEDEMIHPEGVASLLPPDFQSSLERPELELSRHSPRKSSSIS
  EEKGDSDDEKPRKGERRSSRVRQARAAKLSEGSQPAEEEEDQETPSRNLRVRADRNLKTEEEEEEEEEEE
  EDDEEEEGDDEGQKSREAPILKEFKEEGEEIPRVKPEEMMDERPKTRSQEQEVLERGGRFTRSQEEARKS
  HLARQQQEKEMKTTSPLEEEEREIKSSQGLKEKSKSPSPPRLTEDRKKASLVALPEQTASEEETPPPLLT
  KEASSPPPHPQLHSEEEIEPMEGPAPPVLIQLSPPNTDADTRELLVSQHTVQLVGGLSPLSSPSDTKAES
  PAEKVPEESVLPLVQKSTLADYSAQKDLEPESDRSAQPLPLKIEELALAKGITEECLKQPSLEQKEGRRA
  SHTLLPSHRLKQSADSSSSRSSSSSSSSSRSRSRSPDSSGSRSHSPLRSKQRDVAQARTHANPRGRPKMG
  SRSTSESRSRSRSRSRSASSNSRKSLSPGVSRDSSTSYTETKDPSSGQEVATPPVPQLQVCEPKERTSTS
  SSSVQARRLSQPESAEKHVTQRLQPERGSPKKCEAEEAEPPAATQPQTSETQTSHLPESERIHHTVEEKE
  EVTMDTSENRPENDVPEPPMPIADQVSNDDRPEGSVEDEEKKESSLPKSFKRKISVVSTKGVPAGNSDTE
  GGQPGRKRRWGASTATTQKKPSISITTESLKEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVTQ
  VVPAEGQENGQREEEEEEKEPEAEPPVPPQVSVEVALPPPAEHEVKKVTLGDTLTRRSISQQKSGVSITI
  DDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAV
  ATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRA
  EQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQ
  EEPPAKLLDDLFRKTKAAPCIYWLPLTDSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNR
  QLEREKRREHSRERDRERERERERDRGDRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164814
• ORF Size: 3984 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164814.1, NP_001158286.1
• RefSeq ORF: 3987
• Locus ID: 22985
• Protein Pathways: Spliceosome
• MW: 150.4 kDa
• Gene Summary: Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. It may thus be involved in mRNA metabolism associated with splicing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]