IGSF1 (NM_001170963) Human Tagged ORF Clone

CAT#: RC229752

IGSF1 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 1 (IGSF1), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IGSF1
• Synonyms: CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229752 representing NM_001170963
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGGACAGACCAGGGGAGGGGGCCACCATGCTGAAGACATTCACTGTTTTGCTCTTTTGCATTC
  GGATGAGTCTGGGTATGACATCGATAGTGATGGACCCTCAACCGGAGTTGTGGATAGAGTCCAACTACCC
  CCAGGCCCCTTGGGAGAACATCACGCTTTGGTGCCGAAGCCCCTCTCGGATATCAAGCAAGTTCCTGCTG
  CTGAAGGATAAGACACAGATGACCTGGATCCGCCCTTCCCACAAGACCTTCCAAGTTTCATTCCTTATAG
  GTGCCCTTACTGAGTCCAATGCAGGTCTTTACCGGTGCTGCTACTGGAAGGAGACAGGCTGGTCAAAGCC
  CAGTAAAGTTCTAGAGTTGGAGGCACCAGGCCAACTGCCCAAGCCCATCTTCTGGATTCAGGCTGAGACC
  CCCGCTCTTCCTGGGTGTAATGTTAACATCCTCTGCCATGGCTGGCTGCAGGATTTGGTATTCATGCTGT
  TTAAAGAGGGATATGCAGAGCCTGTGGATTACCAAGTCCCAACTGGGACAATGGCCATATTCTCCATTGA
  CAACCTGACACCTGAGGATGAAGGGGTTTACATCTGCCGCACTCATATCCAGATGCTCCCCACCCTGTGG
  TCAGAGCCCAGCAACCCCCTGAAGCTGGTTGTAGCAGGTGGGTGTGGCTATGGCTGCTGGCATCTGGCAA
  TTGTTGTCCCAGGTATCATGGCTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229752 representing NM_001170963
  Red=Cloning site Green=Tags(s)
  
  MTLDRPGEGATMLKTFTVLLFCIRMSLGMTSIVMDPQPELWIESNYPQAPWENITLWCRSPSRISSKFLL
  LKDKTQMTWIRPSHKTFQVSFLIGALTESNAGLYRCCYWKETGWSKPSKVLELEAPGQLPKPIFWIQAET
  PALPGCNVNILCHGWLQDLVFMLFKEGYAEPVDYQVPTGTMAIFSIDNLTPEDEGVYICRTHIQMLPTLW
  SEPSNPLKLVVAGGCGYGCWHLAIVVPGIMAG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170963
• ORF Size: 726 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170963.1, NP_001164434.1
• RefSeq ORF: 729 bp
• Locus ID: 3547
• Cytogenetics: Xq26.1
• Protein Families: Transmembrane
• MW: 27.7 kDa
• Gene Summary: 'This gene encodes a member of the immunoglobulin-like domain-containing superfamily. Proteins in this superfamily contain varying numbers of immunoglobulin-like domains and are thought to participate in the regulation of interactions between cells. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]'