TAF7L (NM_001168474) Human Tagged ORF Clone

CAT#: RC229964

TAF7L (Myc-DDK-tagged)-Human TAF7-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50kDa (TAF7L), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY432964) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229964 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF7L
• Synonyms: CT40; TAF2Q
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229964 representing NM_001168474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAAAGCCAGGATGAAGTTCCTGATGAAGTTGAGAACCAGTTTATATTGCGTCTGCCTCTGGAAC
  ATGCTTGTACTGTCAGGAACCTAGCACGTTCTCAAAGTGTCAAGATGAAGGATAAACTAAAAATTGACTT
  ATTGCCTGATGGGCGCCATGCAGTTGTTGAAGTAGAAGATGTCCCACTAGCTGCTAAGCTGGTTGACTTG
  CCTTGTGTTATTGAAAGCCTGAGAACGCTTGATAAAAAAACCTTTTATAAAACAGCAGACATTTCTCAGA
  TGCTTGTGTGCACTGCTGATGGTGATATCCACCTTTCTCCAGAAGAACCAGCTGCCTCTACTGATCCTAA
  TATAGTCAGGAAAAAAGAAAGGGGGAGAGAAGAAAAATGTGTCTGGAAGCATGGCATTACGCCACCACTT
  AAGAATGTCAGAAAGAAAAGGTTCCGGAAAACACAAAAAAAGGTCCCTGATGTCAAAGAAATGGAAAAAA
  GCAGCTTTACTGAGTACATTGAATCTCCAGACGTGGAAAATGAAGTAAAGAGACTGCTGCGTTCGGATGC
  TGAAGCCGTAAGTACCCGTTGGGAAGTCATTGCTGAAGATGGAACCAAGGAAATAGAAAGTCAAGGCTCC
  ATCCCAGGATTTTTGATATCCTCGGGAATGAGCAGCCACAAGCAGGGTCATACCTCGTCAGAATATGATA
  TGCTTCGGGAGATGTTCAGTGATTCTAGAAGTAACAATGATGATGATGAGGATGAGGATGATGAAGATGA
  GGATGAGGATGAGGATGAGGATGAAGATGAAGACAAAGAAGAGGAGGAGGAAGATTGTTCTGAAGAGTAT
  CTGGAAAGGCAGCTGCAGGCCGAGTTTATTGAATCTGGCCAGTATAGGGCAAATGAAGGTACCAGTTCAA
  TAGTCATGGAAATTCAGAAGCAGATTGAGAAAAAGGAGAAAAAGCTCCATAAGATTCAGAATAAAGCACA
  AAGACAGAAGGATCTCATCATGAAAGTGGAAAACCTGACACTCAAGAATCATTTTCAGTCTGTGCTGGAG
  CAGCTTGAGTTACAGGAAAAACAAAAAAATGAGAAGCTCATTTCCCTACAGGAACAGTTGCAGCGTTTTC
  TGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229964 representing NM_001168474
  Red=Cloning site Green=Tags(s)
  
  MSESQDEVPDEVENQFILRLPLEHACTVRNLARSQSVKMKDKLKIDLLPDGRHAVVEVEDVPLAAKLVDL
  PCVIESLRTLDKKTFYKTADISQMLVCTADGDIHLSPEEPAASTDPNIVRKKERGREEKCVWKHGITPPL
  KNVRKKRFRKTQKKVPDVKEMEKSSFTEYIESPDVENEVKRLLRSDAEAVSTRWEVIAEDGTKEIESQGS
  IPGFLISSGMSSHKQGHTSSEYDMLREMFSDSRSNNDDDEDEDDEDEDEDEDEDEDEDKEEEEEDCSEEY
  LERQLQAEFIESGQYRANEGTSSIVMEIQKQIEKKEKKLHKIQNKAQRQKDLIMKVENLTLKNHFQSVLE
  QLELQEKQKNEKLISLQEQLQRFLKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168474
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001168474.1, NP_001161946.1
• RefSeq ORF: 1131
• Locus ID: 54457
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 44 kDa
• Gene Summary: This gene is similar to a mouse gene that encodes a TATA box binding protein-associated factor, and shows testis-specific expression. The encoded protein could be a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]