Asparagine synthetase (ASNS) (NM_001178077) Human Tagged ORF Clone

CAT#: RC230145

ASNS (Myc-DDK-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASNS
• Synonyms: ASNSD; TS11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230145 representing NM_001178077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGGTGAGATAATCCTTCATCTTTATGACA
  AAGGAGGAATTGAGCAAACAATTTGTATGTTGGATGGTGTGTTTGCATTTGTTTTACTGGATACTGCCAA
  TAAGAAAGTGTTCCTGGGTAGAGATACATATGGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGA
  TTTTTGGCTGTATGTTCAGAAGCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAG
  TGGAGCCTTTTCTTCCTGGACACTATGAAGTTTTGGATTTAAAGCCAAATGGCAAAGTTGCATCCGTGGA
  AATGGTTAAATATCATCACTGTCGGGATGTACCCCTGCACGCCCTCTATGACAATGTGGAGAAACTCTTT
  CCAGGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGGATCCTTTTTAATAATGCTGTAAAGAAACGTT
  TGATGACAGACAGAAGGATTGGCTGCCTTTTATCAGGGGGCTTGGACTCCAGCTTGGTTGCTGCCACTCT
  GTTGAAGCAGCTGAAAGAAGCCCAAGTACAGTATCCTCTCCAGACATTTGCAATTGGCATGGAAGACAGC
  CCCGATTTACTGGCTGCTAGAAAGGTGGCAGATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACT
  CTGAGGAAGGCATTCAGGCTCTGGATGAAGTCATATTTTCCTTGGAAACTTATGACATTACAACAGTTCG
  TGCTTCAGTAGGTATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGATAGCGTGGTGATCTTCTCT
  GGAGAAGGATCAGATGAACTTACGCAGGGTTACATATATTTTCACAAGGCTCCTTCTCCTGAAAAAGCCG
  AGGAGGAGAGTGAGAGGCTTCTGAGGGAACTCTATTTGTTTGATGTTCTCCGCGCAGATCGAACTACTGC
  TGCCCATGGTCTTGAACTGAGAGTCCCATTTCTAGATCATCGATTTTCTTCCTATTACTTGTCTCTGCCA
  CCAGAAATGAGAATTCCAAAGAATGGGATAGAAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATC
  TGATACCCAAAGAGATTCTCTGGCGACCAAAAGAAGCCTTCAGTGATGGAATAACTTCAGTTAAGAATTC
  CTGGTTTAAGATTTTACAGGAATACGTTGAACATCAGGTTGATGATGCAATGATGGCAAATGCAGCCCAG
  AAATTTCCCTTCAATACTCCTAAAACCAAAGAAGGATATTACTACCGTCAAGTCTTTGAACGCCATTACC
  CAGGCCGGGCTGACTGGCTGAGCCATTACTGGATGCCCAAGTGGATCAATGCCACTGACCCTTCTGCCCG
  CACGCTGACCCACTACAAGTCAGCTGTCAAAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230145 representing NM_001178077
  Red=Cloning site Green=Tags(s)
  
  MQQHFEFEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDG
  FLAVCSEAKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLF
  PGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDS
  PDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFS
  GEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLP
  PEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQ
  KFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178077
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178077.1, NP_001171548.1
• RefSeq Size: 1709 bp
• RefSeq ORF: 1437 bp
• Locus ID: 440
• Cytogenetics: 7q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• MW: 54.8 kDa
• Gene Summary: 'The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]'