HHAT (NM_001170580) Human Tagged ORF Clone

CAT#: RC230170

HHAT (Myc-DDK-tagged)-Human hedgehog acyltransferase (HHAT), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HHAT
• Synonyms: MART2; SKI1; Skn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230170 representing NM_001170580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCGATGGGAACTGGCACTTTACCTACTTGCCTCACTAGGCTTCCACTTCTATTCCTTCTATG
  AAGTTTACAAAGTCTCCAGAGAACACGAAGAGGAGCTGGACCAGGAATTTGAGCTGGAGACTGACACTTT
  ATTTGGAGGATTAAAGAAGGATGCGACCGACTTTGAGTGGAGCTTCTGGATGGAATGGGGGAAGCAGTGG
  CTGGTGTGGCTTCTCCTTGGCCACATGGTAGTGTCTCAAATGGCCACACTGCTGGCAAGAAAGCACAGAC
  CCTGGATTCTCATGCTCTATGGGATGTGGGCCTGCTGGTGTGTGCTGGGGACCCCTGGTGTGGCTATGGT
  TTTGCTCCATACCACCATCTCTTTCTGCGTGGCCCAGTTCCGGTCTCAGCTCCTGACGTGGCTCTGTTCT
  CTCCTCCTCCTCTCCACACTGAGGCTGCAGGGTGTGGAAGAAGTTAAGAGAAGGTGGTACAAGACAGAAA
  ACGAGTACTACCTGCTGCAGTTCACGCTGACCGTTCGCTGCCTGTACTACACCAGCTTCAGCCTGGAGCT
  CTGCTGGCAGCAGCTGCCTGCTGCATCGACCTCCTACTCCTTTCCCTGGATGCTGGCCTATGTCTTTTAT
  TATCCAGTCTTACACAATGGGCCCATCCTCAGCTTCTCGGAGTTCATCAAACAGATGCAGCAGCAGGAGC
  ATGACTCCCTGAAGGCCAGCCTGTGTGTCCTGGCCCTGGGGCTGGGCCGCCTTCTTTGCTGGTGGTGGCT
  GGCCGAGCTGATGGCTCACCTGATGTACATGCATGCCATCTACAGCAGCATCCCCCTCCTGGAGACTGTC
  TCTTGTTGGACCTTAGGAGGACTGGCGTTAGCCCAGGTGCTCTTTTTCTACGTGAAGTACTTGGTGCTCT
  TTGGCGTGCCTGCTCTGCTCATGCGCCTGGATGGACTCACTCCACCCGCCCTCCCCCGCTGCGTGAGCAC
  CATGTTCAGTTTCACCGGGATGTGGAGGTATTTTGATGTTGGACTGCATAATTTCTTAATCAGGTATGTG
  TACATTCCAGTGGGCGGGTCCCAGCATGGCCTGCTGGGGACACTGTTTTCCACGGCGATGACATTTGCAT
  TTGTGAGCTACTGGCATGGCGGCTACGACTACCTCTGGTGCTGGGCAGCGCTCAACTGGCTGGGAGTCAC
  TGTGGAGAATGGAGTCCGGAGGCTGGTGGAGACTCCCTGCATCCAGGACAGTCTGGCCCGATACTTCTCC
  CCACAAGCTCGCCGTCGATTCCACGCTGCCCTTGCTTCTTGTTCCACCTCGATGCTGATCCTGTCCAACC
  TGGTATTTCTTGGGGGCAATGAGGTTGGGAAAACCTACTGGAATAGGATCTTCATACAAGGCTGGCCTTG
  GGTGACCCTCTCTGTCCTGGGATTCCTGTACTGCTACTCCCACGTGGGCATTGCCTGGGCCCAGACCTAC
  GCCACGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230170 representing NM_001170580
  Red=Cloning site Green=Tags(s)
  
  MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEWSFWMEWGKQW
  LVWLLLGHMVVSQMATLLARKHRPWILMLYGMWACWCVLGTPGVAMVLLHTTISFCVAQFRSQLLTWLCS
  LLLLSTLRLQGVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQQLPAASTSYSFPWMLAYVFY
  YPVLHNGPILSFSEFIKQMQQQEHDSLKASLCVLALGLGRLLCWWWLAELMAHLMYMHAIYSSIPLLETV
  SCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNFLIRYV
  YIPVGGSQHGLLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVENGVRRLVETPCIQDSLARYFS
  PQARRRFHAALASCSTSMLILSNLVFLGGNEVGKTYWNRIFIQGWPWVTLSVLGFLYCYSHVGIAWAQTY
  ATD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170580
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170580.1, NM_001170580.2, NP_001164051.1
• RefSeq ORF: 1482
• Locus ID: 55733
• Protein Families: Transmembrane
• MW: 57.8 kDa
• Gene Summary: 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725). [supplied by OMIM, Jul 2002]