HHAT (NM_001170587) Human Tagged ORF Clone

CAT#: RC230171

HHAT (Myc-DDK-tagged)-Human hedgehog acyltransferase (HHAT), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HHAT
• Synonyms: MART2; SKI1; Skn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230171 representing NM_001170587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTGGGACTCGGAAGTGCCGAAAGAGGGGTGTTGGGAACTCGCGGCGCGCGTGAACGTTGCCGTC
  GCCGCCGCCCGGGACAGCCCGGAGAACACGAAGAGGAGCTGGACCAGGAATTTGAGCTGGAGACTGACAC
  TTTATTTGGAGGATTAAAGAAGGATGCGACCGACTTTGAGTGGAGCTTCTGGATGGAATGGGGGAAGCAG
  TGGCTGGTGTGGCTTCTCCTTGGCCACATGGTAGTGTCTCAAATGGCCACACTGCTGGCAAGAAAGCACA
  GACCCTGGATTCTCATGCTCTATGGGATGTGGGCCTGCTGGTGTGTGCTGGGGACCCCTGGTGTGGCTAT
  GGTTTTGCTCCATACCACCATCTCTTTCTGCGTGGCCCAGTTCCGGTCTCAGCTCCTGACGTGGCTCTGT
  TCTCTCCTCCTCCTCTCCACACTGAGGCTGCAGGGTGTGGAAGAAGTTAAGAGAAGGTGGTACAAGACAG
  AAAACGAGTACTACCTGCTGCAGTTCACGCTGACCGTTCGCTGCCTGTACTACACCAGCTTCAGCCTGGA
  GCTCTGCTGGCAGCAGCTGCCTGCTGCATCGACCTCCTACTCCTTTCCCTGGATGCTGGCCTATGTCTTT
  TATTATCCAGTCTTACACAATGGGCCCATCCTCAGCTTCTCGGAGTTCATCAAACAGATGCAGCAGCAGG
  AGCATGACTCCCTGAAGGCCAGCCTGTGTGTCCTGGCCCTGGGGCTGGGCCGCCTTCTTTGCTGGTGGTG
  GCTGGCCGAGCTGATGGCTCACCTGATGTACATGCATGCCATCTACAGCAGCATCCCCCTCCTGGAGACT
  GTCTCTTGTTGGACCTTAGGAGGACTGGCGTTAGCCCAGGTGCTCTTTTTCTACGTGAAGTACTTGGTGC
  TCTTTGGCGTGCCTGCTCTGCTCATGCGCCTGGATGGACTCACTCCACCCGCCCTCCCCCGCTGCGTGAG
  CACCATGTTCAGTTTCACCGGGATGTGGAGGTATTTTGATGTTGGACTGCATAATTTCTTAATCAGGTAT
  GTGTACATTCCAGTGGGCGGGTCCCAGCATGGCCTGCTGGGGACACTGTTTTCCACGGCGATGACATTTG
  CATTTGTGAGCTACTGGCATGGCGGCTACGACTACCTCTGGTGCTGGGCAGCGCTCAACTGGCTGGGAGT
  CACTGTGGAGAATGGAGTCCGGAGGCTGGTGGAGACTCCCTGCATCCAGGACAGTCTGGCCCGATACTTC
  TCCCCACAAGCTCGCCGTCGATTCCACGCTGCCCTTGCTTCTTGTTCCACCTCGATGCTGATCCTGTCCA
  ACCTGGTATTTCTTGGGGGCAATGAGGTTGGGAAAACCTACTGGAATAGGATCTTCATACAAGGCTGGCC
  TTGGGTGACCCTCTCTGTCCTGGGATTCCTGTACTGCTACTCCCACGTGGGCATTGCCTGGGCCCAGACC
  TACGCCACGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230171 representing NM_001170587
  Red=Cloning site Green=Tags(s)
  
  MSLGLGSAERGVLGTRGARERCRRRRPGQPGEHEEELDQEFELETDTLFGGLKKDATDFEWSFWMEWGKQ
  WLVWLLLGHMVVSQMATLLARKHRPWILMLYGMWACWCVLGTPGVAMVLLHTTISFCVAQFRSQLLTWLC
  SLLLLSTLRLQGVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQQLPAASTSYSFPWMLAYVF
  YYPVLHNGPILSFSEFIKQMQQQEHDSLKASLCVLALGLGRLLCWWWLAELMAHLMYMHAIYSSIPLLET
  VSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNFLIRY
  VYIPVGGSQHGLLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVENGVRRLVETPCIQDSLARYF
  SPQARRRFHAALASCSTSMLILSNLVFLGGNEVGKTYWNRIFIQGWPWVTLSVLGFLYCYSHVGIAWAQT
  YATD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170587
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170587.1, NM_001170587.2, NP_001164058.1
• RefSeq ORF: 1485
• Locus ID: 55733
• Protein Families: Transmembrane
• MW: 57.4 kDa
• Gene Summary: 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725). [supplied by OMIM, Jul 2002]