Adracalin (AAAS) (NM_001173466) Human Tagged ORF Clone

CAT#: RC230210

AAAS (Myc-DDK-tagged)-Human achalasia, adrenocortical insufficiency, alacrimia (AAAS), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY433210) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230210 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AAAS
• Synonyms: AAA; AAASb; ADRACALA; ADRACALIN; ALADIN; GL003
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230210 representing NM_001173466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCTCTGGGGTTGTTCCCTCCTCCACCGCCTCGGGGTCAAGTCACCCTATATGAGCACAATAACG
  AGCTGGTGACGGGCAGTAGCTATGAGAGCCCGCCCCCCGACTTCCGGGGCCAGTGGATCAATCTTCCTGT
  CCTACAACTGACAAAGGATCCCCTAAAGACCCCTGGAAGGCTGGACCATGGCACAAGAACTGCCTTCATC
  CATCACCGGGAGCAAGTGTGGAAGAGATGCATCAACATTTGGCGTGATGTGGGCCTTTTTGGGGTGCTAA
  ATGAAATTGCAAACTCAGAAGAAGAGGTGTTTGAGTGGGTGAAGACGGCATCCGGCTGGGCCCTGGCACT
  CTGTCGATGGGCCTCTTCCCTCCATGGGTCCCTGTTCCCCCATCTGTCTCTCAGGAGCGAAGATCTGATC
  GCTGAATTTGCCCAAGTCACAAATTGCACCATAGTCCCCTCCCTGAAGCACCGGCTGCAGCGAAATGTGG
  CGTCTCTGGCCTGGAAGCCCCTTAGTGCCTCTGTCTTGGCTGTGGCCTGCCAGAGCTGCATTCTTATCTG
  GACCCTGGACCCTACCTCCTTGTCTACCCGACCCTCTTCTGGCTGTGCCCAAGTGCTGTCTCACCCTGGG
  CATACACCTGTTACCAGCTTGGCCTGGGCCCCCAGTGGGGGGCGGCTGCTCTCAGCTTCACCCGTGGATG
  CTGCTATCCGGGTATGGGATGTCTCAACAGAGACCTGTGTCCCCCTTCCCTGGTTCCGAGGAGGTGGGGT
  GACCAACCTGCTCTGGTCCCCAGACGGCAGCAAAATCCTGGCTACCACTCCTTCAGCTGTCTTTCGAGTC
  TGGGAGGCCCAGATGTGGACTTGTGAGAGGTGGCCTACTCTATCAGGGCGCTGTCAGACTGGCTGCTGGA
  GCCCAGATGGCAGCCGACTGCTGTTCACTGTATTGGGAGAGCCACTGATTTACTCCCTGTCTTTTCCAGA
  ACGTTGTGGTGAGGGAAAGGGGTGCGTTGGAGGTGCAAAGTCAGCAACGATTGTGGCAGATCTGTCTGAG
  ACAACAATACAGACACCAGATGGTGAGGAGAGGCTTGGGGGAGAGGCTCACTCCATGGTCTGGGACCCCA
  GTGGGGAACGTCTGGCTGTGCTTATGAAAGGAAAGCCAAGGGTACAGGATGGTAAACCAGTCATCCTCCT
  TTTTCGCACTCGAAACAGCCCTGTGTTTGAGCTCCTTCCCTGTGGCATTATCCAGGGGGAGCCAGGAGCC
  CAGCCCCAGCTCATCACTTTCCATCCTTCCTTCAACAAAGGGGCCCTGCTCAGTGTGGGCTGGTCCACAG
  GCCGAATTGCCCACATCCCGCTGTACTTTGTCAATGCCCAGTTTCCACGTTTTAGCCCAGTGCTTGGGCG
  GGCCCAGGAACCCCCTGCTGGGGGTGGAGGCTCTATTCATGACCTGCCCCTCTTTACTGAGACATCCCCA
  ACCTCTGCCCCTTGGGACCCTCTCCCAGGGCCACCACCTGTTCTGCCCCACTCCCCACATTCCCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230210 representing NM_001173466
  Red=Cloning site Green=Tags(s)
  
  MCSLGLFPPPPPRGQVTLYEHNNELVTGSSYESPPPDFRGQWINLPVLQLTKDPLKTPGRLDHGTRTAFI
  HHREQVWKRCINIWRDVGLFGVLNEIANSEEEVFEWVKTASGWALALCRWASSLHGSLFPHLSLRSEDLI
  AEFAQVTNCTIVPSLKHRLQRNVASLAWKPLSASVLAVACQSCILIWTLDPTSLSTRPSSGCAQVLSHPG
  HTPVTSLAWAPSGGRLLSASPVDAAIRVWDVSTETCVPLPWFRGGGVTNLLWSPDGSKILATTPSAVFRV
  WEAQMWTCERWPTLSGRCQTGCWSPDGSRLLFTVLGEPLIYSLSFPERCGEGKGCVGGAKSATIVADLSE
  TTIQTPDGEERLGGEAHSMVWDPSGERLAVLMKGKPRVQDGKPVILLFRTRNSPVFELLPCGIIQGEPGA
  QPQLITFHPSFNKGALLSVGWSTGRIAHIPLYFVNAQFPRFSPVLGRAQEPPAGGGGSIHDLPLFTETSP
  TSAPWDPLPGPPPVLPHSPHSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173466
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001173466.1, NP_001166937.1
• RefSeq ORF: 1542
• Locus ID: 8086
• MW: 56.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]