MEPE (NM_001184694) Human Tagged ORF Clone

CAT#: RC230233

MEPE (Myc-DDK-tagged)-Human matrix extracellular phosphoglycoprotein (MEPE), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MEPE
• Synonyms: OF45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230233 representing NM_001184694
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGTTTTCTGTGTGGGACTACTCCTTTTCAGTGTGACCTGGGCAGCACCAACATTTCAACCACAGA
  CTGAGAAAACTAAGCAAAGCTGTGTGGAAGAGCAGAGGCAGGAAGAAAAAAACAAAGACAATATTGGTTT
  TCACCATTTGGGCAAGAGAATAAATCAAGAGCTATCATCTAAAGAAAATATTGTCCAGGAAAGAAAGAAA
  GATTTGTCCCTTTCTGAAGCCAGTGAGAATAAGGGAAGTAGTAAATCTCAAAATTATTTCACAAATAGAC
  AGAGACTGAATAAAGAATATAGTATCAGTAACAAAGAGAATACTCACAATGGCCTGAGGATGTCAATTTA
  TCCTAAGTCAACTGGGAATAAAGGGTTTGAGGATGGAGATGATGCTATCAGCAAACTACATGACCAAGAA
  GAATATGGCGCAGCTCTCATCAGAAATAACATGCAACATATAATGGGGCCAGTGACTGCAATTAAACTCC
  TGGGGGAAGAAAACAAAGAGAACACACCTAGGAATGTTCTAAACATAATCCCAGCAAGTATGAATTATGC
  TAAAGCACACTCGAAGGATAAAAAGAAGCCTCAAAGAGATTCCCAAGCCCAGAAAAGTCCAGTAAAAAGC
  AAAAGCACCCATCGTATTCAACACAACATTGACTACCTAAAACATCTCTCAAAAGTCAAAAAAATCCCCA
  GTGATTTTGAAGGCAGCGGTTATACAGATCTTCAAGAGAGAGGGGACAATGATATATCTCCTTTCAGTGG
  GGACGGCCAACCTTTTAAGGACATTCCTGGTAAAGGAGAAGCTACTGGTCCTGACCTAGAAGGCAAAGAT
  ATTCAAACAGGGTTTGCAGGCCCAAGTGAAGCTGAGAGTACTCATCTTGACACAAAAAAGCCAGGTTATA
  ATGAGATCCCAGAGAGAGAAGAAAATGGTGGAAATACCATTGGAACTAGGGATGAAACTGCGAAAGAGGC
  AGATGCTGTTGATGTCAGCCTTGTAGAGGGCAGCAACGATATCATGGGTAGTACCAATTTTAAGGAGCTC
  CCTGGAAGAGAAGGAAACAGAGTGGATGCTGGCAGCCAAAATGCTCACCAAGGGAAGGTTGAGTTTCATT
  ACCCTCCTGCACCCTCAAAAGAGAAAAGAAAAGAAGGCAGTAGTGATGCAGCTGAAAGTACCAACTATAA
  TGAAATTCCTAAAAATGGCAAAGGCAGTACCAGAAAGGGTGTAGATCATTCTAATAGGAACCAAGCAACC
  TTAAATGAAAAACAAAGGTTTCCTAGTAAGGGCAAAAGTCAGGGCCTGCCCATTCCTTCTCGTGGTCTTG
  ATAATGAAATCAAAAACGAAATGGATTCCTTTAATGGCCCCAGTCATGAGAATATAATAACACATGGCAG
  AAAATATCATTATGTACCCCACAGACAAAATAATTCTACACGGAATAAGGGTATGCCACAAGGGAAAGGC
  TCCTGGGGTAGACAACCCCATTCCAACAGGAGGTTTAGTTCCCGTAGAAGGGATGACAGTAGTGAGTCAT
  CTGACAGTGGCAGTTCAAGTGAGAGCGATGGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230233 representing NM_001184694
  Red=Cloning site Green=Tags(s)
  
  MRVFCVGLLLFSVTWAAPTFQPQTEKTKQSCVEEQRQEEKNKDNIGFHHLGKRINQELSSKENIVQERKK
  DLSLSEASENKGSSKSQNYFTNRQRLNKEYSISNKENTHNGLRMSIYPKSTGNKGFEDGDDAISKLHDQE
  EYGAALIRNNMQHIMGPVTAIKLLGEENKENTPRNVLNIIPASMNYAKAHSKDKKKPQRDSQAQKSPVKS
  KSTHRIQHNIDYLKHLSKVKKIPSDFEGSGYTDLQERGDNDISPFSGDGQPFKDIPGKGEATGPDLEGKD
  IQTGFAGPSEAESTHLDTKKPGYNEIPEREENGGNTIGTRDETAKEADAVDVSLVEGSNDIMGSTNFKEL
  PGREGNRVDAGSQNAHQGKVEFHYPPAPSKEKRKEGSSDAAESTNYNEIPKNGKGSTRKGVDHSNRNQAT
  LNEKQRFPSKGKSQGLPIPSRGLDNEIKNEMDSFNGPSHENIITHGRKYHYVPHRQNNSTRNKGMPQGKG
  SWGRQPHSNRRFSSRRRDDSSESSDSGSSSESDGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184694
• ORF Size: 1575 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001184694.1, NM_001184694.2, NP_001171623.1
• RefSeq ORF: 1578
• Locus ID: 56955
• Protein Families: Secreted Protein
• MW: 58.9 kDa
• Gene Summary: This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]