ARMCX5 (NM_001168479) Human Tagged ORF Clone

CAT#: RC230270

ARMCX5 (Myc-DDK-tagged)-Human armadillo repeat containing, X-linked 5 (ARMCX5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARMCX5
• Synonyms: GASP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230270 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGACTCTGGGACAGAAGCAAGGGCTAGAGGAAAGGCTGAGGCTGGCCTGCAAGATGGAATCAGTG
  GTCCTGCCACTGCTAGAGTGAATGGTAAAACCCAGGCCGAGGCAGTGGCTGAGGCAGAACTGAAAACAGA
  ATCAGTGACCCAGGCCAAAGCTGGTGATGGAGCAATGACCAGGACACATACAGTGACCTACAGGGAGGCT
  ATGGCTGTGACAAGGGAAGTGATCAAGGTGGAAGATACAACTAAGACTAGAGTCATGGTTGAGACTAAGA
  CAAAACCCCTGGCAGAACGCAGTATAGTGCCACAAACCAAGTCAAAGGCCATGCCTATGTCTAGGGTCAG
  TACTGTAACCAAGTCTGAAGTCAAGGTTGTTGCTGTCATTGAGGCAAATATTAGGTCCTATGCCAAGTCA
  CATGATAAGGCCAATACTGGGTCCAGACCTGACAGAAGGGAAGAGACCAGCATTGGGATGAAATCCAGTG
  ATGAGGATGAAGAAAATATATGCTCCTGGTTCTGGACTGGAGAAGAGCCTAGTGTAGGGTCCTGGTTCTG
  GCCTGAAGAAGAGACCTCTCTTCAAGTTTATAAGCCCCTACCTAAGATCCAGGAAAAGCCCAAGCCCACA
  CACAAACCCACACTTACTATAAAACAAAAGGTAATAGCATGGTCAAGGGCCAGGTATATTGTCCTAGTTC
  CAGTTGAAGGAGGGGAGCAATCCTTGCCTCCAGAAGGAAACTGGACCCTGGTTGAGACCTTGATTGAAAC
  TCCTCTGGGGATTCGACCTTTGACCAAGATCCCACCTTATCATGGGCCTTATTACCAGACCTTAGCTGAG
  ATCAAAAAACAGATTAGGCAAAGGGAAAAGTATGGGCCTAATCCGAAGGCCTGCCACTGCAAATCACGTG
  GCTTTAGTTTAGAGCCTAAAGAGTTTGATAAACTTGTTGCCCTCCTTAAGTTAACTAAGGATCCTTTCAT
  TCATGAAATAGCTACAATGATAATGGGCATCAGTCCTGCTTATCCATTTACTCAAGATATAATTCATGAT
  GTAGGTATTACTGTTATGATTGAAAACTTGGTCAATAATCCCAATGTTAAAGAACACCCTGGAGCTTTAA
  GTATGGTGGATGACAGCTCTGAGTCTTCCGAAGAACCAAAATCAGGGGAGTCATATATACATCAAGTTTG
  TAAAGGCATAATCTCTTGCCCCTTGAACTCCCCTGTGCAGCTGGCTGGACTGAAATTACTAGGGCACTTG
  AGTATAAAATTTGAAGATCACTATGTGATTACCAGTTATATTCCAGATTTCCTCACCTTGTTAAACAAGG
  GAAGTGTCAAAACCAAGTTTTATGTTTTAAAAGTGTTTTCGTGTTTGTCTAAAAATCACGCCAATACAAG
  AGAATTGATCAGTGCCAAAGTACTGTCATCATTGGTTGCACCCTTTAACAAGAATGAGTCAAAGGCCAAT
  ATTCTTAATATTATTGAAATATTTGAGAATATAAATTTTCAGTTCAAAACAAAGGCAAAGCTATTTACCA
  AGGAAAAGTTCACTAAATCTGAGCTTATTTCAATATTCCAGGAAGCAAAACAGTTTGGTCAGAAACTCCA
  AGACTTAGCAGAGCACAGTGATCCCGAAGTGAGAGATAAAGTCATACGATTAATACTAAAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230270 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVDSGTEARARGKAEAGLQDGISGPATARVNGKTQAEAVAEAELKTESVTQAKAGDGAMTRTHTVTYREA
  MAVTREVIKVEDTTKTRVMVETKTKPLAERSIVPQTKSKAMPMSRVSTVTKSEVKVVAVIEANIRSYAKS
  HDKANTGSRPDRREETSIGMKSSDEDEENICSWFWTGEEPSVGSWFWPEEETSLQVYKPLPKIQEKPKPT
  HKPTLTIKQKVIAWSRARYIVLVPVEGGEQSLPPEGNWTLVETLIETPLGIRPLTKIPPYHGPYYQTLAE
  IKKQIRQREKYGPNPKACHCKSRGFSLEPKEFDKLVALLKLTKDPFIHEIATMIMGISPAYPFTQDIIHD
  VGITVMIENLVNNPNVKEHPGALSMVDDSSESSEEPKSGESYIHQVCKGIISCPLNSPVQLAGLKLLGHL
  SIKFEDHYVITSYIPDFLTLLNKGSVKTKFYVLKVFSCLSKNHANTRELISAKVLSSLVAPFNKNESKAN
  ILNIIEIFENINFQFKTKAKLFTKEKFTKSELISIFQEAKQFGQKLQDLAEHSDPEVRDKVIRLILKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168479
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168479.1, NP_001161951.1
• RefSeq Size: 2924 bp
• RefSeq ORF: 1677 bp
• Locus ID: 64860
• MW: 62.3 kDa