GGA1 (NM_001172688) Human Tagged ORF Clone

CAT#: RC230284

GGA1 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 5

Product Images

Western blot validation of overexpression lysate (Cat# LY433284) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230284 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230284 representing NM_001172688
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGCTGCGGCAAGCGGTTCCACGACGAAGTGGGCAAGTTCCGCTTTCTCAACGAGCTCATCAAGG
  TCGTGTCTCCCAAGTATCTGGGCTCTCGGACATCGGAGAAGGTGAAGAACAAGATCTTGGAGCTCCTCTA
  CAGCTGGACAGTGGGCCTGCCCGAGGAGGTGAAAATCGCAGAGGCCTACCAGATGCTAAAGAAGCAGGGG
  ATTGTAAAGTCCGACCCCAAGCTTCCAGATGACACTACCTTTCCCCTTCCTCCTCCACGGCCGAAGAATG
  TGATCTTTGAAGATGAGGAGAAATCCAAGATGCTGGCCCGCCTGCTGAAGAGCTCCCATCCCGAAGACCT
  CCGCGCAGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGGATGGAGAAGATCTCGAAG
  AGGGTGAATGCCATCGAGGAGGTGAACAACAATGTGAAACTGCTCACGGAGATGGTGATGAGCCACAGCC
  AGGGCGGCGCAGCAGCTGGCAGCAGCGAGGACCTCATGAAGGAACTGTACCAGCGCTGTGAGCGGATGCG
  GCCCACGCTCTTCCGACTGGCGAGTGACACAGAGGACAATGATGAGGCCTTAGCGGAGATCCTGCAGGCC
  AATGACAACCTCACCCAGGTGATCAACCTGTATAAGCAGCTGGTGCGGGGTGAGGAGGTCAACGGTGATG
  CCACAGCCGGCTCCATCCCTGGGAGCACCTCGGCCCTGCTGGATCTCTCAGGCCTGGATCTCCCGCCTGC
  GGGCACCACCTACCCAGCTATGCCCACCCGCCCTGGCGAGCAGGCCAGCCCTGAGCAGCCCAGTGCCTCA
  GTTTCCCTGCTTGACGACGAGCTCATGTCTCTGGGCCTCAGTGACCCCACACCCCCTTCAGGCCCAAGCC
  TGGATGGTACCGGATGGAACAGCTTCCAGTCGTCGGATGCCACTGAGCCCCCAGCCCCTGCTCTGGCCCA
  GGCCCCCAGTATGGAAAGCCGACCCCCAGCGCAGACATCCCTGCCAGCAAGCAGCGGTCTGGACGACCTA
  GACCTCCTGGGGAAGACCCTCCTGCAGCAGTCGCTGCCCCCGGAATCCCAGCAAGTGCGGTGGGAGAAGC
  AGCAGCCAACCCCCCGGCTCACACTCCGGGACCTGCAGAATAAGAGCAGCAGCTGCAGCTCCCCCAGCTC
  CAGCGCCACCAGCCTTCTCCACACCGTGTCCCCAGAGCCCCCCAGGCCTCCGCAGCAGCCCGTACCAACC
  GAGCTCTCACTGGCCAGCATCACTGTGCCCCTGGAGTCCATCAAACCCAGCAACATCCTGCCCGTGACTG
  TGTATGACCAGCACGGCTTCCGCATCCTCTTCCATTTTGCCCGGGACCCACTGCCAGGGCGCTCCGACGT
  GCTGGTGGTGGTGGTTTCCATGCTGAGCACCGCCCCCCAGCCCATCCGCAACATCGTGTTCCAGTCAGCT
  GTCCCCAAGGTTATGAAGGTGAAGCTGCAGCCACCCTCGGGCACGGAGCTGCCAGCTTTTAACCCCATCG
  TCCACCCCTCAGCAATCACCCAGGTCCTGCTGCTTGCCAACCCCCAGAAGGAGAAGGTTCGCCTCCGCTA
  CAAGCTCACCTTCACCATGGGTGACCAGACCTACAACGAGATGGGGGATGTGGACCAGTTCCCCCCACCT
  GAAACCTGGGGTAGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230284 representing NM_001172688
  Red=Cloning site Green=Tags(s)
  
  MKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQG
  IVKSDPKLPDDTTFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISK
  RVNAIEEVNNNVKLLTEMVMSHSQGGAAAGSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEILQA
  NDNLTQVINLYKQLVRGEEVNGDATAGSIPGSTSALLDLSGLDLPPAGTTYPAMPTRPGEQASPEQPSAS
  VSLLDDELMSLGLSDPTPPSGPSLDGTGWNSFQSSDATEPPAPALAQAPSMESRPPAQTSLPASSGLDDL
  DLLGKTLLQQSLPPESQQVRWEKQQPTPRLTLRDLQNKSSSCSSPSSSATSLLHTVSPEPPRPPQQPVPT
  ELSLASITVPLESIKPSNILPVTVYDQHGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSA
  VPKVMKVKLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQTYNEMGDVDQFPPP
  ETWGSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172688
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001172688.1, NP_001166159.1
• RefSeq ORF: 1701
• Locus ID: 26088
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 62.6 kDa
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]