Adducin 2 (ADD2) (NM_001185055) Human Tagged ORF Clone
CAT#: RC230299
- TrueORF®
ADD2 (Myc-DDK-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 6
"NM_001185055" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ADD2 |
Synonyms | ADDB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC230299 representing NM_001185055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGCAGGAGAGTTCCAGGAGCGAATTGCAAACCCACCGGGAAAATGAGCGAAGAGACGGTCCCCG AGGCTGCCTCGCCGCCGCCCCCGCAGGGGCAGCCTTACTTTGACCGCTTCTCAGAGGACGACCCCGAGTA CATGCGCCTTCGCAACCGGGCGGCGGACCTGCGGCAGGACTTCAACCTGATGGAGCAGAAGAAGCGCGTC ACCATGATCCTGCAGAGTCCCTCTTTCAGGGAGGAGCTGGAAGGCCTCATCCAGGAGCAGATGAAGAAGG GGAACAACTCCTCCAACATCTGGGCCCTGCGACAGATCGCGGACTTCATGGCCAGCACCTCCCACGCAGT CTTCCCGACATCTTCCATGAATGTCTCCATGATGACGCCTATCAATGACCTCCACACAGCTGACTCCCTG AACCTGGCCAAAGGGGAGCGGCTCATGCGGTGCAAGATCAGCAGTGTCTACCGACTCCTGGACCTCTATG GCTGGGCCCAGCTGAGTGACACCTATGTCACGTTGAGAGTCAGCAAGGAGCAGGACCACTTCCTGATCAG CCCTAAGGGAGTTTCTTGCAGTGAAGTCACAGCGTCCAGCCTGATCAAGGTGAACATTCTGGGAGAGGTG GTGGAGAAGGGCAGCAGCTGCTTCCCAGTGGACACCACAGGCTTCTGTCTGCACTCGGCCATCTATGCAG CGAGGCCCGACGTGCGCTGCATCATCCACCTGCACACACCGGCCACAGCAGCGGTGTCGGCCATGAAGTG GGGCCTCCTGCCTGTCTCCCACAATGCCCTGCTGGTGGGGGACATGGCCTATTATGACTTCAATGGGGAA ATGGAGCAGGAAGCCGATCGGATCAACCTGCAGAAGTGCCTTGGACCCACCTGCAAGATCCTGGTGCTAA GAAACCATGGAGTGGTTGCTCTGGGTGACACGGTAGAGGAGGCATTTTACAAGATCTTCCACCTGCAGGC TGCATGTGAGATACAGGTGTCGGCTCTGTCCAGTGCCGGGGGAGTGGAGAACCTCATCCTCCTGGAGCAG GAGAAGCACCGGCCCCATGAGGTGGGCTCCGTGCAGTGGGCCGGGAGCACCTTTGGGCCTATGCAGAAGA GTCGGCTGGGGGAGCATGAGTTTGAGGCCCTCATGAGGATGCTGGACAACCTGGGCTACAGAACAGGTTA CACGTATCGCCACCCCTTTGTTCAAGAGAAAACCAAACACAAAAGTGAGGTGGAGATTCCAGCCACGGTC ACAGCCTTCGTGTTTGAGGAGGACGGTGCCCCGGTGCCCGCCCTGCGACAGCATGCCCAGAAGCAGCAGA AGGAGAAGACCCGCTGGCTCAATACGCCCAACACCTACCTGCGGGTCAATGTGGCCGATGAGGTCCAGAG GAGCATGGGCAGCCCCCGACCCAAGACCACGTGGATGAAGGCTGACGAGGTGGAGAAATCCAGCAGTGGC ATGCCGATTCGCATCGAAAACCCAAACCAATTTGTGCCTCTCTATACTGACCCCCAGGAAGTACTGGAGA TGAGGAACAAGATTCGAGAACAAAACCGACAAGATGTGAAGTCAGCGGGGCCTCAGTCCCAGCTCCTGGC GAGCGTCATTGCCGAGAAGAGCCGAAGCCCGGTAGAGCAGAGGCTGCCCCTGACTGGCGGGGAAACGTGT TTGCCTTCGGGGTCTTCTGTGCCTGGGGCTGGGTTGCAGGACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230299 representing NM_001185055
Red=Cloning site Green=Tags(s) MPRRRVPGANCKPTGKMSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRV TMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSL NLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEV VEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGE MEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQ EKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATV TAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSG MPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPVEQRLPLTGGETC LPSGSSVPGAGLQDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001185055 |
ORF Size | 1725 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001185055.1, NP_001171984.1 |
RefSeq ORF | 1728 bp |
Locus ID | 119 |
Cytogenetics | 2p13.3 |
MW | 64.7 kDa |
Gene Summary | 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC328937 | ADD2 (untagged)-Human adducin 2 (beta) (ADD2) transcript variant 6 |
USD 970.00 |
|
RG230299 | ADD2 (GFP-tagged) - Human adducin 2 (beta) (ADD2), transcript variant 6 |
USD 580.00 |
|
RC230299L3 | Lenti-ORF clone of ADD2 (Myc-DDK-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 6 |
USD 730.00 |
|
RC230299L4 | Lenti-ORF clone of ADD2 (mGFP-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 6 |
USD 730.00 |
{0} Product Review(s)
Be the first one to submit a review