Adducin 2 (ADD2) (NM_001185055) Human Untagged Clone

CAT#: SC328937

ADD2 (untagged)-Human adducin 2 (beta) (ADD2) transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADD2
• Synonyms: ADDB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001185055, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGCGCAGGAGAGTTCCAGGAGCGAATTGCAAACCCACCGGGAAAATGAGCGAAGAGACGGTCCCCG
  AGGCTGCCTCGCCGCCGCCCCCGCAGGGGCAGCCTTACTTTGACCGCTTCTCAGAGGACGACCCCGAGTA
  CATGCGCCTTCGCAACCGGGCGGCGGACCTGCGGCAGGACTTCAACCTGATGGAGCAGAAGAAGCGCGTC
  ACCATGATCCTGCAGAGTCCCTCTTTCAGGGAGGAGCTGGAAGGCCTCATCCAGGAGCAGATGAAGAAGG
  GGAACAACTCCTCCAACATCTGGGCCCTGCGACAGATCGCGGACTTCATGGCCAGCACCTCCCACGCAGT
  CTTCCCGACATCTTCCATGAATGTCTCCATGATGACGCCTATCAATGACCTCCACACAGCTGACTCCCTG
  AACCTGGCCAAAGGGGAGCGGCTCATGCGGTGCAAGATCAGCAGTGTCTACCGACTCCTGGACCTCTATG
  GCTGGGCCCAGCTGAGTGACACCTATGTCACGTTGAGAGTCAGCAAGGAGCAGGACCACTTCCTGATCAG
  CCCTAAGGGAGTTTCTTGCAGTGAAGTCACAGCGTCCAGCCTGATCAAGGTGAACATTCTGGGAGAGGTG
  GTGGAGAAGGGCAGCAGCTGCTTCCCAGTGGACACCACAGGCTTCTGTCTGCACTCGGCCATCTATGCAG
  CGAGGCCCGACGTGCGCTGCATCATCCACCTGCACACACCGGCCACAGCAGCGGTGTCGGCCATGAAGTG
  GGGCCTCCTGCCTGTCTCCCACAATGCCCTGCTGGTGGGGGACATGGCCTATTATGACTTCAATGGGGAA
  ATGGAGCAGGAAGCCGATCGGATCAACCTGCAGAAGTGCCTTGGACCCACCTGCAAGATCCTGGTGCTAA
  GAAACCATGGAGTGGTTGCTCTGGGTGACACGGTAGAGGAGGCATTTTACAAGATCTTCCACCTGCAGGC
  TGCATGTGAGATACAGGTGTCGGCTCTGTCCAGTGCCGGGGGAGTGGAGAACCTCATCCTCCTGGAGCAG
  GAGAAGCACCGGCCCCATGAGGTGGGCTCCGTGCAGTGGGCCGGGAGCACCTTTGGGCCTATGCAGAAGA
  GTCGGCTGGGGGAGCATGAGTTTGAGGCCCTCATGAGGATGCTGGACAACCTGGGCTACAGAACAGGTTA
  CACGTATCGCCACCCCTTTGTTCAAGAGAAAACCAAACACAAAAGTGAGGTGGAGATTCCAGCCACGGTC
  ACAGCCTTCGTGTTTGAGGAGGACGGTGCCCCGGTGCCCGCCCTGCGACAGCATGCCCAGAAGCAGCAGA
  AGGAGAAGACCCGCTGGCTCAATACGCCCAACACCTACCTGCGGGTCAATGTGGCCGATGAGGTCCAGAG
  GAGCATGGGCAGCCCCCGACCCAAGACCACGTGGATGAAGGCTGACGAGGTGGAGAAATCCAGCAGTGGC
  ATGCCGATTCGCATCGAAAACCCAAACCAATTTGTGCCTCTCTATACTGACCCCCAGGAAGTACTGGAGA
  TGAGGAACAAGATTCGAGAACAAAACCGACAAGATGTGAAGTCAGCGGGGCCTCAGTCCCAGCTCCTGGC
  GAGCGTCATTGCCGAGAAGAGCCGAAGCCCGGTAGAGCAGAGGCTGCCCCTGACTGGCGGGGAAACGTGT
  TTGCCTTCGGGGTCTTCTGTGCCTGGGGCTGGGTTGCAGGACCCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001185055
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001185055.1, NP_001171984.1
• RefSeq Size: 3707 bp
• RefSeq ORF: 1728 bp
• Locus ID: 119
• Cytogenetics: 2p13.3
• Gene Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an upstream start codon, compared to variant 1. This variant also differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (f) has a distinct N-terminus and is shorter than isoform a.