SIGLEC10 (NM_001171156) Human Tagged ORF Clone

CAT#: RC230382

SIGLEC10 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 10 (SIGLEC10), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY433382) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230382 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC10
• Synonyms: PRO940; SIGLEC-10; SLG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230382 representing NM_001171156
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACTGCCACTGCTGCTGTCCTCGCTGCTGGGCGGGTCCCAGGCTATGGATGGGAGATTCTGGATAC
  GAGTGCAGGAGTCAGTGATGGTGCCGGAGGGCCTGTGCATCTCTGTGCCCTGCTCTTTCTCCTACCCCCG
  ACAAGACTGGACAGGGTCTACCCCAGCTTATGGCTACTGGTTCAAAGCAGTGACTGAGACAACCAAGGGT
  GCTCCTGTGGCCACAAACCACCAGAGTCGAGAGGTGGAAATGAGCACCCGGGGCCGATTCCAGCTCACTG
  GGGATCCCGCCAAGGGGAACTGCTCCTTGGTGATCAGAGACGCGCAGATGCAGGATGAGTCACAGTACTT
  CTTTCGGGTGGAGAGAGGAAGCTATGTGAGATATAATTTCATGAACGATGGGTTCTTTCTAAAAGTAACA
  GTGCTCAGCTTCACGCCCAGACCCCAGGACCACAACACCGACCTCACCTGCCATGTGGACTTCTCCAGAA
  AGGGTGTGAGCGCACAGAGGACCGTCCGACTCCGTGTGGCCTATGCCCCCAGAGACCTTGTTATCAGCAT
  TTCACGTGACAACACGCCAGCCCTGGAGCCCCAGCCCCAGGGAAATGTCCCATACCTGGAAGCCCAAAAA
  GGCCAGTTCCTGCGGCTCCTCTGTGCTGCTGACAGCCAGCCCCCTGCCACACTGAGCTGGGTCCTGCAGA
  ACAGAGTCCTCTCCTCGTCCCATCCCTGGGGCCCTAGACCCCTGGGGCTGGAGCTGCCCGGGGTGAAGGC
  TGGGGATTCAGGGCGCTACACCTGCCGAGCGGAGAACAGGCTTGGCTCCCAGCAGCGAGCCCTGGACCTC
  TCTGTGCAGTATCCTCCAGAGAACCTGAGAGTGATGGTTTCCCAAGCAAACAGGACAGTCCTGGAAAACC
  TTGGGAACGGCACGTCTCTCCCAGTACTGGAGGGCCAAAGCCTGTGCCTGGTCTGTGTCACACACAGCAG
  CCCCCCAGCCAGGCTGAGCTGGACCCAGAGGGGACAGGTTCTGAGCCCCTCCCAGCCCTCAGACCCCGGG
  GTCCTGGAGCTGCCTCGGGTTCAAGTGGAGCACGAAGGAGAGTTCACCTGCCACGCTCGGCACCCACTGG
  GCTCCCAGCACGTCTCTCTCAGCCTCTCCGTGCACTACTCCCCGAAGCTGCTGGGCCCCTCCTGCTCCTG
  GGAGGCTGAGGGTCTGCACTGCAGCTGCTCCTCCCAGGCCAGCCCGGCCCCCTCTCTGCGCTGGTGGCTT
  GGGGAGGAGCTGCTGGAGGGGAACAGCAGCCAGGACTCCTTCGAGGTCACCCCCAGCTCAGCCGGGCCCT
  GGGCCAACAGCTCCCTGAGCCTCCATGGAGGGCTCAGCTCCGGCCTCAGGCTCCGCTGTGAGGCCTGGAA
  CGTCCATGGGGCCCAGAGTGGATCCATCCTGCAGCTGCCAGATAAGAAGGGACTCATCTCAACGGCATTC
  TCCAACGGAGCGTTTCTGGGAATCGGCATCACGGCTCTTCTTTTCCTCTGCCTGGCCCTGATCATCATGA
  AGATTCTACCGAAGAGACGGACTCAGACAGAAACCCCGAGGCCCAGGTTCTCCCGGCACAGCACGATCCT
  GGATTACATCAATGTGGTCCCGACGGCTGGCCCCCTGGCTCAGAAGCGGAATCAGAAAGCCACACCAAAC
  AGTCCTCGGACCCCTCTTCCACCAGGTGCTCCCTCCCCAGAATCAAAGAAGAACCAGAAAAAGCAGTATC
  AGTTGCCCAGTTTCCCAGAACCCAAATCATCCACTCAAGCCCCAGAATCCCAGGAGAGCCAAGAGGAGCT
  CCATTATGCCACGCTCAACTTCCCAGGCGTCAGACCCAGGCCTGAGGCCCGGATGCCCAAGGGCACCCAG
  GCGGATTATGCAGAAGTCAAGTTCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230382 representing NM_001171156
  Red=Cloning site Green=Tags(s)
  
  MLLPLLLSSLLGGSQAMDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKG
  APVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVT
  VLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQK
  GQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDL
  SVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPG
  VLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWL
  GEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLISTAF
  SNGAFLGIGITALLFLCLALIIMKILPKRRTQTETPRPRFSRHSTILDYINVVPTAGPLAQKRNQKATPN
  SPRTPLPPGAPSPESKKNQKKQYQLPSFPEPKSSTQAPESQESQEELHYATLNFPGVRPRPEARMPKGTQ
  ADYAEVKFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171156
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001171156.1, NP_001164627.1
• RefSeq ORF: 1920
• Locus ID: 89790
• Protein Families: Druggable Genome, Transmembrane
• MW: 70.8 kDa
• Gene Summary: SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405). [supplied by OMIM, Jul 2002]