ZNF185 (NM_001178106) Human Tagged ORF Clone

CAT#: RC230456

ZNF185 (Myc-DDK-tagged)-Human zinc finger protein 185 (LIM domain) (ZNF185), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF185
• Synonyms: SCELL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230456 representing NM_001178106
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTATCTCAGCTCTTGGAGGCCGCACCAAAGGGAAGCCTCTGCCACCAGGCGAGGAGGAGCGCAATA
  ACGTTCTCAAGCAGATGAAAGTGCGAACCACGCTGAAGGGGGACAAGAGCTGGATTACCAAGCAGGATGA
  ATCGGAGGGTCGCACCATAGAGCTGCCCTCAGGCCGGAGTCGCGCCACATCCTTTTCATCAGCTGGGGAG
  GTTCCGAAGCCTAGGCCTCCGAGCACAAGGGCTCCCACTGGCTACATCATCCGGGGAGTGTTCACCAAGC
  CCATAGACAGCTCTTCCCAGCCCCAGCAGCAGTTCCCCAAGGCCAACGGGACTCCAAAAAGTGCTGCCAG
  TCTGGTGAGAACAGCTAACGCTGGTCCTCCCCGCCCCTCCTCCTCTGGCTACAAGATGACCACTGAGGAT
  TACAAGAAGCTGGCACCCTACAATATCAGGCGCAGCTCTACATCAGGGGACACCGAGGAGGAGGAGGAGG
  AGGAGGTGGTGCCATTCTCCTCAGATGAACAGAAACGGAGGTCAGAGGCTGCAAGCGGTGTTCTGAGGAG
  GACAGCTCCCCGGGAGCACTCCTACGTCCTGTCAGCGGCCAAGAAGAGCACTGGTCCTACCCAGGAGACA
  CAGGCACCGTTTATCGCGAAGAGGGTGGAGGTGGTGGAAGAGGACGGGCCTTCTGAGAAGAGCCAGGACC
  CACCTGCTCTGGCAAGATCCACTCCTGGCTCAAACAGTGCGGATGGAGGCAGGACCAAAGCGTCTCGGGC
  AATTTGGATCGAGTGCCTGCCAAGTATGCCTAGCCCCGCTGGGAGCCAGGAGCTCAGCTCAAGAGGTGAG
  GAAATTGTCCGCCTGCAGATCCTGACACCCAGGGCAGGACTCCGCCTGGTGGCCCCAGACGTGGAAGGCA
  TGAGGTCTTCCCCAGGCAACAAAGACAAGGAGGCCCCCTGCTCCAGAGAGCTCCAGAGGGACTTGGCTGG
  TGAGGAGGCTTTCAGGGCCCCCAACACAGATGCTGCAAGGTCAAGTGCACAGTTGAGTGATGGCAATGTG
  GGATCCGGAGCCACGGGCTCCCGGCCTGAAGGCTTGGCTGCAGTAGACATCGGCTCCGAGAGAGGAAGGC
  TGTGTGCAGCTGCTAGCTTTGCCTCTTTCCTGGAAGACCAGGATGGGCACAGTGCCAACTCTCAGTCCTG
  CAAGCCTAGACCAGCAGCCATCAGCTCCAGTGCCACTTCAGTCTCTGCTGTCCCTGCTGATAGGAAGAGC
  AACAGCACAGCAGCCCAGGAGGATGCAAAGGCAGACCCAAAGGGGGCCTTGGCTGATTATGAGGGGAAGG
  ATGTGGCCACCAGGGTCGGAGAGGCCTGGCAGGAGAGGCCTGGAGCTCCAAGAGGTGGCCAAGGAGACCC
  AGCTGTACCCGCTCAGCAACCTGCAGATCCCAGCACCCCAGAGCGGCAGAGCAGCCCCAGCGGATCTGAG
  CAACTTGTCAGACGAGAGAGTTGTGGCAGCAGCGTGTTGACTGATTTTGAGGGGAAGGATGTGGCCACCA
  AGGTCGGAGAGGCCTGGCAGGACAGGCCTGGAGCCCCAAGAGGTGGCCAAGGAGACCCAGCTGTACCCAC
  TCAGCAACCTGCAGATCCCAGTACCCCAGAACAGCAGAACAGCCCCAGCGGATCTGAGCAATTCGTCAGA
  CGAGAGAGCTGCACCAGCAGGGTGAGGAGCCCCTCGAGCTGCATGGTCACTGTTACTGTCACTGCCACAT
  CTGAGCAGCCTCACATTTATATTCCAGCCCCCGCAAGTGAATTGGACTCCAGCTCTACCACCAAAGGGAT
  TCTCTTCGTGAAGGAGTACGTGAATGCTAGTGAAGTGTCTTCTGGGAAGCCAGTATCTGCACGCTATAGC
  AACGTCAGCAGCATTGAGGACTCATTCGCCATGGAGAAGAAGCCTCCATGTGGCAGCACTCCATACTCTG
  AGAGGACAACTGGAGGGATCTGTACTTACTGCAACCGTGAGATCCGAGACTGTCCAAAGATTACCCTAGA
  ACATCTTGGTATCTGCTGCCATGAATATTGCTTTAAGTGTGGGATTTGCAGTAAACCGATGGGCGATCTC
  CTGGATCAGATCTTCATTCACCGTGACACCATTCACTGTGGGAAATGCTATGAGAAGCTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230456 representing NM_001178106
  Red=Cloning site Green=Tags(s)
  
  MSISALGGRTKGKPLPPGEEERNNVLKQMKVRTTLKGDKSWITKQDESEGRTIELPSGRSRATSFSSAGE
  VPKPRPPSTRAPTGYIIRGVFTKPIDSSSQPQQQFPKANGTPKSAASLVRTANAGPPRPSSSGYKMTTED
  YKKLAPYNIRRSSTSGDTEEEEEEEVVPFSSDEQKRRSEAASGVLRRTAPREHSYVLSAAKKSTGPTQET
  QAPFIAKRVEVVEEDGPSEKSQDPPALARSTPGSNSADGGRTKASRAIWIECLPSMPSPAGSQELSSRGE
  EIVRLQILTPRAGLRLVAPDVEGMRSSPGNKDKEAPCSRELQRDLAGEEAFRAPNTDAARSSAQLSDGNV
  GSGATGSRPEGLAAVDIGSERGRLCAAASFASFLEDQDGHSANSQSCKPRPAAISSSATSVSAVPADRKS
  NSTAAQEDAKADPKGALADYEGKDVATRVGEAWQERPGAPRGGQGDPAVPAQQPADPSTPERQSSPSGSE
  QLVRRESCGSSVLTDFEGKDVATKVGEAWQDRPGAPRGGQGDPAVPTQQPADPSTPEQQNSPSGSEQFVR
  RESCTSRVRSPSSCMVTVTVTATSEQPHIYIPAPASELDSSSTTKGILFVKEYVNASEVSSGKPVSARYS
  NVSSIEDSFAMEKKPPCGSTPYSERTTGGICTYCNREIRDCPKITLEHLGICCHEYCFKCGICSKPMGDL
  LDQIFIHRDTIHCGKCYEKLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178106
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178106.1, NP_001171577.1
• RefSeq ORF: 2166 bp
• Locus ID: 7739
• Cytogenetics: Xq28
• MW: 77.3 kDa
• Gene Summary: 'Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This gene encodes a LIM-domain zinc finger protein. The LIM domain is composed of two contiguous zinc finger domains, separated by a two-amino acid residue hydrophobic linker. The LIM domain mediates protein:protein interactions. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, May 2010]'