BCAR1 (NM_001170720) Human Tagged ORF Clone

CAT#: RC230457

BCAR1 (Myc-DDK-tagged)-Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 8

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCAR1
• Synonyms: CAS; CAS1; CASS1; CRKAS; P130Cas
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230457 representing NM_001170720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGGAAGAACGTGCTGGCCAAAGCGCTCTATGACAATGTGGCCGAGTCCCCGGATGAGCTCTCCT
  TCCGCAAGGGTGACATCATGACGGTGCTGGAGCAGGACACGCAGGGCCTGGACGGCTGGTGGCTCTGCTC
  GCTGCATGGGCGCCAGGGCATCGTGCCTGGGAACCGCCTCAAGATCTTGGTGGTGGTGCCCACCCGCGTG
  GGGCAGGGCTATGTATACGAGGCCGCCCAGCCGGAGCAGGACGAGTACGACATCCCGCGACACCTGCTGG
  CCCCGGGGCCACAGGACATCTATGATGTGCCCCCGGTTCGGGGGCTGCTTCCCAGCCAGTATGGCCAGGA
  GGTGTATGACACACCCCCCATGGCTGTCAAGGGTCCCAATGGCCGAGACCCGTTGCTGGAGGTGTATGAC
  GTGCCCCCCAGTGTGGAGAAGGGCCTGCCACCGTCCAACCACCACGCAGTCTACGACGTTCCTCCATCGG
  TGAGCAAGGATGTGCCCGATGGCCCACTGCTGCGTGAGGAGACCTACGATGTGCCCCCCGCCTTCGCCAA
  GGCCAAGCCCTTTGACCCGGCCCGCACCCCACTGGTACTGGCTGCGCCCCCTCCAGACTCCCCGCCGGCC
  GAGGACGTGTATGACGTGCCGCCCCCGGCTCCTGACCTCTACGACGTGCCCCCTGGCTTGCGGCGGCCTG
  GCCCGGGCACCCTGTACGATGTGCCCCGTGAACGGGTGCTTCCTCCTGAGGTGGCTGATGGTGGCGTGGT
  CGACAGTGGTGTGTATGCGGTGCCTCCCCCAGCTGAACGTGAAGCCCCGGCAGAGGGCAAGCGCCTGTCG
  GCCTCCAGCACCGGCAGCACACGCAGCAGCCAGTCTGCGTCCTCCTTGGAGGTGGCAGGGCCGGGCCGGG
  AACCCCTGGAGCTGGAAGTTGCTGTGGAGGCCCTGGCACGGCTGCAGCAGGGTGTGAGCGCCACCGTTGC
  CCACCTTCTGGACCTGGCAGGCAGCGCCGGTGCGACTGGGAGCTGGCGTAGCCCCTCTGAGCCACAGGAG
  CCGCTGGTGCAGGACCTGCAGGCTGCTGTGGCCGCTGTCCAGAGTGCCGTCCACGAGCTGTTGGAGTTTG
  CCCGCAGCGCGGTGGGCAATGCTGCCCACACATCTGACCGTGCCCTGCATGCCAAGCTTAGCCGGCAGCT
  GCAGAAGATGGAGGACGTGCACCAGACGCTGGTGGCACATGGTCAGGCCCTCGACGCTGGCCGGGGAGGC
  TCTGGAGCCACCCTTGAGGACCTGGACCGGCTGGTGGCCTGCTCGCGGGCTGTGCCCGAGGACGCCAAGC
  AGCTGGCCTCCTTCCTGCACGGCAATGCCTCACTGCTCTTCAGACGGACCAAGGCCACTGCCCCGGGGCC
  TGAGGGGGGTGGCACCCTGCACCCCAACCCCACTGACAAGACCAGCAGCATCCAGTCACGACCCCTGCCC
  TCACCCCCTAAGTTCACCTCCCAGGACTCGCCAGATGGGCAGTACGAGAACAGCGAGGGGGGCTGGATGG
  AGGACTATGACTACGTCCACCTACAGGGGAAGGAGGAGTTTGAGAAGACCCAGAAGGAGCTGCTGGAAAA
  GGGCAGCATCACGCGGCAGGGCAAGAGCCAGCTGGAGTTGCAGCAGCTGAAGCAGTTTGAACGACTGGAA
  CAGGAGGTGTCACGGCCCATAGACCACGACCTGGCCAACTGGACGCCAGCCCAACCCCTGGCCCCGGGGC
  GAACAGGCGGCCTGGGGCCCTCGGACCGGCAGCTGCTGCTCTTCTACCTGGAGCAGTGTGAGGCCAACCT
  GACCACACTGACCAACGCCGTGGACGCCTTCTTTACCGCCGTGGCCACCAACCAGCCGCCCAAGATCTTT
  GTGGCGCACAGCAAGTTCGTCATCCTCAGCGCCCACAAGCTGGTGTTCATCGGGGACACACTGTCACGGC
  AGGCCAAGGCTGCTGACGTGCGCAGCCAGGTGACCCACTACAGCAACCTGCTGTGCGACCTCCTGCGCGG
  CATCGTGGCCACCACCAAGGCCGCTGCCTTGCAGTACCCATCGCCTTCCGCGGCCCAGGACATGGTGGAG
  AGGGTCAAGGAGCTGGGCCACAGCACCCAGCAGTTCCGCCGCGTCCTAGGCCAGCTGGCAGCCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230457 representing NM_001170720
  Red=Cloning site Green=Tags(s)
  
  MQGKNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVVVPTRV
  GQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYD
  VPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPA
  EDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLS
  ASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQE
  PLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGG
  SGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLP
  SPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLE
  QEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIF
  VAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVE
  RVKELGHSTQQFRRVLGQLAAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170720
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170720.1, NP_001164191.1
• RefSeq ORF: 2169
• Locus ID: 9564
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• MW: 78.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]